Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           21     229
NP_000170                                                              21     229
NP_001268422                                                           21     229
NP_001268423                                                           21     229
XP_005264328                                                           21     229
NP_004645                                                              18    1482
NP_001034680                                                           18    1482
NP_001034679                                                           18    1482
XP_005272733                                                           18    1482
XP_005272732                                                           18    1482
NP_055527                                                              17    2528
XP_006721170                                                           17    2528
XP_006721169                                                           17    2528
XP_005255780                                                           17    2528
NP_689778                                                              17    3301
NP_073622                                                              16    5631
NP_060903                                                              16    5631
NP_001140160                                                           16    5631
NP_001059                                                              16    5631
XP_006724360                                                           16    5631
XP_006724359                                                           16    5631
XP_005261762                                                           16    5631
XP_005261761                                                           16    5631
XP_005265484                                                           16    5631
NP_942153                                                              16    5631
NP_003025                                                              16    7355
NP_055402                                                              16    7355
NP_115553                                                              16    7355
XP_005253527                                                           16    7355
NP_001167535                                                           15    9605
NP_005393                                                              15    9605
NP_001167534                                                           15    9605
NP_001167536                                                           15    9605
NP_001167537                                                           15    9605
NP_005912                                                              15    9605
NP_056934                                                              15    9605
NP_075598                                                              15    9605
NP_075594                                                              15    9605
NP_075593                                                              15    9605
NP_001276980                                                           15    9605
NP_001276979                                                           15    9605
NP_001276981                                                           15    9605
NP_037414                                                              15    9605
NP_001041666                                                           15    9605
NP_055068                                                              15    9605
NP_001156750                                                           15    9605
NP_001167538                                                           15    9605
NP_001156751                                                           15    9605
NP_899068                                                              15    9605
NP_055240                                                              15    9605
XP_006724788                                                           15    9605
XP_005251980                                                           15    9605
XP_006715826                                                           15    9605
XP_006715825                                                           15    9605
>NP_001268421
          Length = 1230

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GNGALKRKS 9
           GNG+LKRKS
Sbjct: 176 GNGSLKRKS 184
>NP_000170
          Length = 1360

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GNGALKRKS 9
           GNG+LKRKS
Sbjct: 306 GNGSLKRKS 314
>NP_001268422
          Length = 1058

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  GNGALKRKS 9
          GNG+LKRKS
Sbjct: 4  GNGSLKRKS 12
>NP_001268423
          Length = 1058

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  GNGALKRKS 9
          GNG+LKRKS
Sbjct: 4  GNGSLKRKS 12
>XP_005264328
          Length = 1261

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GNGALKRKS 9
           GNG+LKRKS
Sbjct: 207 GNGSLKRKS 215
>NP_004645
          Length = 2555

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    GNGALKRK 8
            GNG LKRK
Sbjct: 2408 GNGDLKRK 2415
>NP_001034680
          Length = 2554

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    GNGALKRK 8
            GNG LKRK
Sbjct: 2407 GNGDLKRK 2414
>NP_001034679
          Length = 2570

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    GNGALKRK 8
            GNG LKRK
Sbjct: 2407 GNGDLKRK 2414
>XP_005272733
          Length = 2559

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    GNGALKRK 8
            GNG LKRK
Sbjct: 2412 GNGDLKRK 2419
>XP_005272732
          Length = 2575

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    GNGALKRK 8
            GNG LKRK
Sbjct: 2412 GNGDLKRK 2419
>NP_055527
          Length = 1707

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GNGALKRKS 9
            G GAL+R+S
Sbjct: 1385 GEGALRRRS 1393
>XP_006721170
          Length = 1707

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GNGALKRKS 9
            G GAL+R+S
Sbjct: 1385 GEGALRRRS 1393
>XP_006721169
          Length = 1707

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GNGALKRKS 9
            G GAL+R+S
Sbjct: 1385 GEGALRRRS 1393
>XP_005255780
          Length = 1707

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GNGALKRKS 9
            G GAL+R+S
Sbjct: 1385 GEGALRRRS 1393
>NP_689778
          Length = 350

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   NGALKRKS 9
           NGAL++KS
Sbjct: 114 NGALQKKS 121
>NP_073622
          Length = 1501

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GNGALKRK 8
           GNG L+R+
Sbjct: 863 GNGILRRR 870
>NP_060903
          Length = 1321

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GALKRKS 9
           GA+KRKS
Sbjct: 258 GAVKRKS 264
>NP_001140160
          Length = 1321

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GALKRKS 9
           GA+KRKS
Sbjct: 258 GAVKRKS 264
>NP_001059
          Length = 1621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    GNGALKRKS 9
            G GA KRK+
Sbjct: 1536 GRGAKKRKA 1544
>XP_006724360
          Length = 885

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GNGALKRK 8
           GNG L+R+
Sbjct: 161 GNGILRRR 168
>XP_006724359
          Length = 1424

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GNGALKRK 8
           GNG L+R+
Sbjct: 863 GNGILRRR 870
>XP_005261762
          Length = 1481

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GNGALKRK 8
           GNG L+R+
Sbjct: 757 GNGILRRR 764
>XP_005261761
          Length = 1587

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GNGALKRK 8
           GNG L+R+
Sbjct: 863 GNGILRRR 870
>XP_005265484
          Length = 1626

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    GNGALKRKS 9
            G GA KRK+
Sbjct: 1541 GRGAKKRKA 1549
>NP_942153
          Length = 1349

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GNGALKRK 8
           GNG L+R+
Sbjct: 711 GNGILRRR 718
>NP_003025
          Length = 356

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GNGALKRKS 9
           GNG + +KS
Sbjct: 142 GNGGILKKS 150
>NP_055402
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  GNGALKRK 8
          G GALK K
Sbjct: 92 GGGALKAK 99
>NP_115553
          Length = 394

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   GNGALKRK 8
           G G LKRK
Sbjct: 193 GQGHLKRK 200
>XP_005253527
          Length = 205

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GNGALKRKS 9
           GNG + +KS
Sbjct: 142 GNGGILKKS 150
>NP_001167535
          Length = 812

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGALKRK 8
           NG LKR+
Sbjct: 806 NGGLKRR 812
>NP_005393
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   NGALKRKS 9
           NG  KRKS
Sbjct: 187 NGKKKRKS 194
>NP_001167534
          Length = 820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGALKRK 8
           NG LKR+
Sbjct: 814 NGGLKRR 820
>NP_001167536
          Length = 820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGALKRK 8
           NG LKR+
Sbjct: 814 NGGLKRR 820
>NP_001167537
          Length = 733

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGALKRK 8
           NG LKR+
Sbjct: 727 NGGLKRR 733
>NP_005912
          Length = 1512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  GNGALKRKS 9
          G GALK  S
Sbjct: 27 GGGALKASS 35
>NP_056934
          Length = 820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGALKRK 8
           NG LKR+
Sbjct: 814 NGGLKRR 820
>NP_075598
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGALKRK 8
           NG LKR+
Sbjct: 816 NGGLKRR 822
>NP_075594
          Length = 731

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGALKRK 8
           NG LKR+
Sbjct: 725 NGGLKRR 731
>NP_075593
          Length = 733

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGALKRK 8
           NG LKR+
Sbjct: 727 NGGLKRR 733
>NP_001276980
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GNGALKRKS 9
           G G  KRKS
Sbjct: 643 GKGKWKRKS 651
>NP_001276979
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GNGALKRKS 9
           G G  KRKS
Sbjct: 643 GKGKWKRKS 651
>NP_001276981
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GNGALKRKS 9
           G G  KRKS
Sbjct: 643 GKGKWKRKS 651
>NP_037414
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GNGALKRKS 9
           G G  KRKS
Sbjct: 656 GKGKWKRKS 664
>NP_001041666
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GNGALKRKS 9
           G G  KRKS
Sbjct: 643 GKGKWKRKS 651
>NP_055068
          Length = 2725

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    NGALKRKS 9
            NG LKR S
Sbjct: 1615 NGVLKRVS 1622
>NP_001156750
          Length = 2732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    NGALKRKS 9
            NG LKR S
Sbjct: 1622 NGVLKRVS 1629
>NP_001167538
          Length = 853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NGALKRK 8
           NG LKR+
Sbjct: 847 NGGLKRR 853
>NP_001156751
          Length = 2731

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    NGALKRKS 9
            NG LKR S
Sbjct: 1621 NGVLKRVS 1628
>NP_899068
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  GNGALKRK 8
          GN ALK K
Sbjct: 36 GNSALKDK 43
>NP_055240
          Length = 1065

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  GNGALKRK 8
          GN ALK K
Sbjct: 36 GNSALKDK 43
>XP_006724788
          Length = 2570

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    NGALKRKS 9
            NG LKR S
Sbjct: 1460 NGVLKRVS 1467
>XP_005251980
          Length = 1084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  GNGALKRK 8
          GN ALK K
Sbjct: 36 GNSALKDK 43
>XP_006715826
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   NGALKRKS 9
           NG  KRKS
Sbjct: 187 NGKKKRKS 194
>XP_006715825
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   NGALKRKS 9
           NG  KRKS
Sbjct: 187 NGKKKRKS 194
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.126    0.337 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 677,927
Number of extensions: 1308
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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