Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003290                                                              18    1135
XP_005258567                                                           18    1935
NP_789780                                                              18    1935
NP_060960                                                              17    4312
NP_004332                                                              17    4312
XP_005264615                                                           17    4312
XP_006712164                                                           17    4312
XP_005264614                                                           17    4312
XP_005264613                                                           17    4312
XP_005264612                                                           17    4312
NP_001257933                                                           16    5631
NP_038466                                                              16    7355
NP_444295                                                              16    7355
NP_064516                                                              16    7355
NP_038472                                                              16    7355
NP_059509                                                              16    7355
XP_005252005                                                           16    7355
XP_005245405                                                           16    7355
XP_005245404                                                           16    7355
NP_997273                                                              15    9605
NP_001191047                                                           15    9605
NP_001034672                                                           15    9605
NP_001034673                                                           15    9605
NP_036539                                                              15    9605
>NP_003290
          Length = 803

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GMTITLVLK 9
           G TITLVLK
Sbjct: 244 GTTITLVLK 252
>XP_005258567
          Length = 1036

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GMTITLVLK 9
           GMT+ L+LK
Sbjct: 795 GMTVPLILK 803
>NP_789780
          Length = 1043

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GMTITLVLK 9
           GMT+ L+LK
Sbjct: 795 GMTVPLILK 803
>NP_060960
          Length = 951

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GMTITLVL 8
           G T+TLVL
Sbjct: 705 GFTVTLVL 712
>NP_004332
          Length = 2225

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GMTITLV 7
            GMTIT+V
Sbjct: 2072 GMTITMV 2078
>XP_005264615
          Length = 2162

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GMTITLV 7
            GMTIT+V
Sbjct: 2009 GMTITMV 2015
>XP_006712164
          Length = 2179

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GMTITLV 7
            GMTIT+V
Sbjct: 2026 GMTITMV 2032
>XP_005264614
          Length = 2191

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GMTITLV 7
            GMTIT+V
Sbjct: 2038 GMTITMV 2044
>XP_005264613
          Length = 2208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GMTITLV 7
            GMTIT+V
Sbjct: 2055 GMTITMV 2061
>XP_005264612
          Length = 2242

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GMTITLV 7
            GMTIT+V
Sbjct: 2089 GMTITMV 2095
>NP_001257933
          Length = 186

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   GMTITLV 7
           GMTITL+
Sbjct: 168 GMTITLL 174
>NP_038466
          Length = 589

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMTITLVLK 9
           G+T+ LV+K
Sbjct: 99  GLTVHLVIK 107
>NP_444295
          Length = 561

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMTITLVLK 9
           G+T+ LV+K
Sbjct: 99  GLTVHLVIK 107
>NP_064516
          Length = 601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GMTITLVLK 9
          G+T+ LV+K
Sbjct: 75 GLTVHLVIK 83
>NP_038472
          Length = 624

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMTITLVLK 9
           G+T+ LV+K
Sbjct: 95  GLTVHLVIK 103
>NP_059509
          Length = 655

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GMTITLVLK 9
          G+T+ LV+K
Sbjct: 84 GLTVHLVIK 92
>XP_005252005
          Length = 449

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMTITLVLK 9
           G+T+ LV+K
Sbjct: 99  GLTVHLVIK 107
>XP_005245405
          Length = 427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GMTITLVLK 9
          G+T+ LV+K
Sbjct: 75 GLTVHLVIK 83
>XP_005245404
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GMTITLVLK 9
          G+T+ LV+K
Sbjct: 75 GLTVHLVIK 83
>NP_997273
          Length = 306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   GMTITLV 7
           G+T+TL+
Sbjct: 187 GLTVTLI 193
>NP_001191047
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   GMTITLV 7
           G+T+TL+
Sbjct: 187 GLTVTLI 193
>NP_001034672
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMTITLVLK 9
           GM++ +VLK
Sbjct: 111 GMSLDIVLK 119
>NP_001034673
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMTITLVLK 9
           GM++ +VLK
Sbjct: 111 GMSLDIVLK 119
>NP_036539
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMTITLVLK 9
           GM++ +VLK
Sbjct: 111 GMSLDIVLK 119
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.143    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 285,933
Number of extensions: 341
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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