Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001261                                                              22      79
XP_005271924                                                           22      79
XP_005271923                                                           22      79
NP_444507                                                              18    1482
NP_001036037                                                           18    1482
NP_001262                                                              18    1482
XP_006724088                                                           18    1482
NP_003325                                                              17    2528
NP_695012                                                              17    2528
XP_005272707                                                           17    2528
XP_005272706                                                           17    2528
XP_005272705                                                           17    2528
XP_006716621                                                           17    3301
XP_005250973                                                           17    3301
XP_005250972                                                           17    3301
XP_006716620                                                           17    3301
XP_006716619                                                           17    3301
NP_976324                                                              17    3301
NP_001012985                                                           17    4312
NP_003963                                                              17    4312
XP_005270323                                                           17    4312
XP_005270322                                                           17    4312
XP_005263404                                                           17    4312
XP_005263403                                                           17    4312
XP_006714474                                                           17    4312
XP_006714473                                                           17    4312
XP_006714472                                                           17    4312
XP_005263402                                                           17    4312
XP_005263401                                                           17    4312
XP_005263399                                                           17    4312
XP_005263400                                                           17    4312
XP_006714471                                                           17    4312
XP_005263398                                                           17    4312
NP_212132                                                              16    5631
NP_001258929                                                           16    5631
NP_001258930                                                           16    5631
NP_001258928                                                           16    5631
NP_001846                                                              16    5631
NP_008855                                                              16    5631
NP_001071634                                                           16    5631
XP_006722075                                                           16    5631
XP_006711059                                                           16    5631
XP_005271350                                                           16    5631
XP_005271349                                                           16    5631
NP_055317                                                              16    7355
NP_061990                                                              16    7355
NP_689472                                                              16    7355
NP_476503                                                              16    7355
NP_000160                                                              15    9605
NP_005492                                                              15    9605
NP_002195                                                              15    9605
NP_055928                                                              15    9605
NP_001191742                                                           15    9605
NP_002517                                                              15    9605
NP_647479                                                              15    9605
NP_758963                                                              15    9605
NP_001164015                                                           15    9605
NP_758872                                                              15    9605
NP_065744                                                              15    9605
NP_612642                                                              15    9605
NP_001153318                                                           15    9605
NP_001164014                                                           15    9605
NP_002229                                                              15    9605
XP_006722989                                                           15    9605
XP_005259720                                                           15    9605
XP_005257365                                                           15    9605
XP_005256618                                                           15    9605
XP_005256617                                                           15    9605
NP_116257                                                              15    9605
>NP_001261
          Length = 1710

 Score = 22.3 bits (46), Expect =    79
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GMRKLDNYK 9
           GM+KLDNYK
Sbjct: 345 GMKKLDNYK 353
>XP_005271924
          Length = 1710

 Score = 22.3 bits (46), Expect =    79
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GMRKLDNYK 9
           GM+KLDNYK
Sbjct: 345 GMKKLDNYK 353
>XP_005271923
          Length = 1798

 Score = 22.3 bits (46), Expect =    79
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GMRKLDNYK 9
           GM+KLDNYK
Sbjct: 345 GMKKLDNYK 353
>NP_444507
          Length = 686

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MRKLDNY 8
           +RKLDNY
Sbjct: 582 LRKLDNY 588
>NP_001036037
          Length = 501

 Score = 18.1 bits (35), Expect =  1482
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   GMRKLDNYK 9
           G++KL+N+K
Sbjct: 334 GLKKLENFK 342
>NP_001262
          Length = 1828

 Score = 18.1 bits (35), Expect =  1482
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   GMRKLDNYK 9
           G++KL+N+K
Sbjct: 334 GLKKLENFK 342
>XP_006724088
          Length = 646

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MRKLDNY 8
           +RKLDNY
Sbjct: 542 LRKLDNY 548
>NP_003325
          Length = 1058

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           G R+LD+YK
Sbjct: 915 GHRQLDSYK 923
>NP_695012
          Length = 1058

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           G R+LD+YK
Sbjct: 915 GHRQLDSYK 923
>XP_005272707
          Length = 1058

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           G R+LD+YK
Sbjct: 915 GHRQLDSYK 923
>XP_005272706
          Length = 1064

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           G R+LD+YK
Sbjct: 921 GHRQLDSYK 929
>XP_005272705
          Length = 1109

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           G R+LD+YK
Sbjct: 966 GHRQLDSYK 974
>XP_006716621
          Length = 1001

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MRKLDNYK 9
           +R+LDN+K
Sbjct: 534 LRQLDNFK 541
>XP_005250973
          Length = 1001

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MRKLDNYK 9
           +R+LDN+K
Sbjct: 534 LRQLDNFK 541
>XP_005250972
          Length = 1001

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MRKLDNYK 9
           +R+LDN+K
Sbjct: 534 LRQLDNFK 541
>XP_006716620
          Length = 1001

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MRKLDNYK 9
           +R+LDN+K
Sbjct: 534 LRQLDNFK 541
>XP_006716619
          Length = 1001

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MRKLDNYK 9
           +R+LDN+K
Sbjct: 534 LRQLDNFK 541
>NP_976324
          Length = 1001

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MRKLDNYK 9
           +R+LDN+K
Sbjct: 534 LRQLDNFK 541
>NP_001012985
          Length = 1706

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>NP_003963
          Length = 1849

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    RKLDNYK 9
            +KL+NYK
Sbjct: 1251 KKLENYK 1257
>XP_005270323
          Length = 1519

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RKLDNYK 9
           +KL+NYK
Sbjct: 921 KKLENYK 927
>XP_005270322
          Length = 1646

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    RKLDNYK 9
            +KL+NYK
Sbjct: 1048 KKLENYK 1054
>XP_005263404
          Length = 1308

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>XP_005263403
          Length = 1672

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>XP_006714474
          Length = 1674

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>XP_006714473
          Length = 1675

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>XP_006714472
          Length = 1706

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>XP_005263402
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>XP_005263401
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>XP_005263399
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>XP_005263400
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>XP_006714471
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>XP_005263398
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GMRKLDNY 8
           G+RKL++Y
Sbjct: 604 GIRKLEDY 611
>NP_212132
          Length = 2109

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           GM+ +DN+K
Sbjct: 709 GMKWVDNHK 717
>NP_001258929
          Length = 2100

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           GM+ +DN+K
Sbjct: 709 GMKWVDNHK 717
>NP_001258930
          Length = 2098

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           GM+ +DN+K
Sbjct: 709 GMKWVDNHK 717
>NP_001258928
          Length = 2129

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           GM+ +DN+K
Sbjct: 709 GMKWVDNHK 717
>NP_001846
          Length = 1388

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GMRKLDNY 8
            G+R +DNY
Sbjct: 1331 GVRLVDNY 1338
>NP_008855
          Length = 248

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MRKLDNYK 9
           +RKLDN K
Sbjct: 172 VRKLDNTK 179
>NP_001071634
          Length = 201

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MRKLDNYK 9
           +RKLDN K
Sbjct: 172 VRKLDNTK 179
>XP_006722075
          Length = 196

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MRKLDNYK 9
           +RKLDN K
Sbjct: 172 VRKLDNTK 179
>XP_006711059
          Length = 1517

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           GM+ +DN+K
Sbjct: 95  GMKWVDNHK 103
>XP_005271350
          Length = 1854

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           GM+ +DN+K
Sbjct: 709 GMKWVDNHK 717
>XP_005271349
          Length = 2131

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GMRKLDNYK 9
           GM+ +DN+K
Sbjct: 709 GMKWVDNHK 717
>NP_055317
          Length = 504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MRKLDNYK 9
           +RKL N+K
Sbjct: 421 LRKLKNFK 428
>NP_061990
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MRKLDNY 8
           +RK++NY
Sbjct: 169 LRKMENY 175
>NP_689472
          Length = 1910

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KLDNYK 9
           KLDN+K
Sbjct: 948 KLDNFK 953
>NP_476503
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MRKLDNY 8
           +RK++NY
Sbjct: 169 LRKMENY 175
>NP_000160
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GMRKLDNY 8
           G+R+L NY
Sbjct: 116 GIRQLANY 123
>NP_005492
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   GMRKLDNY 8
           G+R LD Y
Sbjct: 587 GLRSLDAY 594
>NP_002195
          Length = 1051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   GMRKLDNY 8
           G+R LD Y
Sbjct: 587 GLRSLDAY 594
>NP_055928
          Length = 2961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    GMRKLD 6
            G+RKLD
Sbjct: 1075 GLRKLD 1080
>NP_001191742
          Length = 524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   KLDNY 8
           KLDNY
Sbjct: 330 KLDNY 334
>NP_002517
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   KLDNY 8
           KLDNY
Sbjct: 330 KLDNY 334
>NP_647479
          Length = 988

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   RKLDNY 8
           RKLD+Y
Sbjct: 336 RKLDHY 341
>NP_758963
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   RKLDNY 8
           RKLD+Y
Sbjct: 336 RKLDHY 341
>NP_001164015
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  KLDNYK 9
          KLD+YK
Sbjct: 88 KLDHYK 93
>NP_758872
          Length = 989

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   RKLDNY 8
           RKLD+Y
Sbjct: 366 RKLDHY 371
>NP_065744
          Length = 2113

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    GMRKLDNY 8
            GM K  NY
Sbjct: 1216 GMEKFTNY 1223
>NP_612642
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  KLDNYK 9
          KLD+YK
Sbjct: 88 KLDHYK 93
>NP_001153318
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RKLDNYK 9
           +KLD YK
Sbjct: 153 QKLDKYK 159
>NP_001164014
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  KLDNYK 9
          KLD+YK
Sbjct: 88 KLDHYK 93
>NP_002229
          Length = 962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   RKLDNY 8
           RKLD+Y
Sbjct: 339 RKLDHY 344
>XP_006722989
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RKLDNYK 9
           +KLD YK
Sbjct: 153 QKLDKYK 159
>XP_005259720
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RKLDNYK 9
           +KLD YK
Sbjct: 153 QKLDKYK 159
>XP_005257365
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   GMRKLDNY 8
           G+R LD Y
Sbjct: 452 GLRSLDAY 459
>XP_005256618
          Length = 2962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    GMRKLD 6
            G+RKLD
Sbjct: 1076 GLRKLD 1081
>XP_005256617
          Length = 2966

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    GMRKLD 6
            G+RKLD
Sbjct: 1076 GLRKLD 1081
>NP_116257
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RKLDNYK 9
           +KLD YK
Sbjct: 153 QKLDKYK 159
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.140    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 563,660
Number of extensions: 875
Number of successful extensions: 69
Number of sequences better than 10000.0: 69
Number of HSP's gapped: 69
Number of HSP's successfully gapped: 69
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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