Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              20     509
XP_006716142                                                           20     509
XP_005250088                                                           20     509
XP_006716141                                                           20     509
XP_005250085                                                           20     509
XP_006716140                                                           20     509
XP_005250084                                                           20     509
XP_005250083                                                           20     509
XP_005250082                                                           20     509
NP_937839                                                              17    2528
XP_005247120                                                           17    2528
XP_006713533                                                           17    2528
XP_006713532                                                           17    2528
NP_005807                                                              17    2528
NP_002378                                                              17    3301
NP_001078846                                                           17    3301
XP_005272048                                                           17    3301
XP_006714680                                                           17    3301
NP_001129386                                                           16    5631
NP_060107                                                              15    9605
XP_006716851                                                           15    9605
XP_006716850                                                           15    9605
XP_006716849                                                           15    9605
XP_006716848                                                           15    9605
XP_006716847                                                           15    9605
>NP_733751
          Length = 4911

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GMLGSEISS 9
            GM+GSEISS
Sbjct: 3435 GMVGSEISS 3443
>XP_006716142
          Length = 4927

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GMLGSEISS 9
            GM+GSEISS
Sbjct: 3452 GMVGSEISS 3460
>XP_005250088
          Length = 4928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GMLGSEISS 9
            GM+GSEISS
Sbjct: 3452 GMVGSEISS 3460
>XP_006716141
          Length = 4959

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GMLGSEISS 9
            GM+GSEISS
Sbjct: 3452 GMVGSEISS 3460
>XP_005250085
          Length = 4979

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GMLGSEISS 9
            GM+GSEISS
Sbjct: 3452 GMVGSEISS 3460
>XP_006716140
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GMLGSEISS 9
            GM+GSEISS
Sbjct: 3452 GMVGSEISS 3460
>XP_005250084
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GMLGSEISS 9
            GM+GSEISS
Sbjct: 3452 GMVGSEISS 3460
>XP_005250083
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GMLGSEISS 9
            GM+GSEISS
Sbjct: 3451 GMVGSEISS 3459
>XP_005250082
          Length = 4983

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GMLGSEISS 9
            GM+GSEISS
Sbjct: 3452 GMVGSEISS 3460
>NP_937839
          Length = 585

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   MLGSEISS 9
           +LGSEISS
Sbjct: 377 LLGSEISS 384
>XP_005247120
          Length = 419

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   MLGSEISS 9
           +LGSEISS
Sbjct: 377 LLGSEISS 384
>XP_006713533
          Length = 568

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   MLGSEISS 9
           +LGSEISS
Sbjct: 361 LLGSEISS 368
>XP_006713532
          Length = 584

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   MLGSEISS 9
           +LGSEISS
Sbjct: 377 LLGSEISS 384
>NP_005807
          Length = 569

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   MLGSEISS 9
           +LGSEISS
Sbjct: 361 LLGSEISS 368
>NP_002378
          Length = 829

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   MLGSEISS 9
           +LGSEISS
Sbjct: 346 VLGSEISS 353
>NP_001078846
          Length = 1019

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   MLGSEISS 9
           +LGSEISS
Sbjct: 536 VLGSEISS 543
>XP_005272048
          Length = 851

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   MLGSEISS 9
           +LGSEISS
Sbjct: 346 VLGSEISS 353
>XP_006714680
          Length = 1002

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   MLGSEISS 9
           +LGSEISS
Sbjct: 497 VLGSEISS 504
>NP_001129386
          Length = 1503

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    GMLGSEIS 8
            G+LGSE++
Sbjct: 1029 GLLGSELA 1036
>NP_060107
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GMLGSEIS 8
            G LG+E+S
Sbjct: 1013 GSLGAEVS 1020
>XP_006716851
          Length = 1008

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GMLGSEIS 8
           G LG+E+S
Sbjct: 922 GSLGAEVS 929
>XP_006716850
          Length = 1121

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GMLGSEIS 8
            G LG+E+S
Sbjct: 1035 GSLGAEVS 1042
>XP_006716849
          Length = 1125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GMLGSEIS 8
            G LG+E+S
Sbjct: 1039 GSLGAEVS 1046
>XP_006716848
          Length = 1127

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GMLGSEIS 8
            G LG+E+S
Sbjct: 1041 GSLGAEVS 1048
>XP_006716847
          Length = 1149

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    GMLGSEIS 8
            G LG+E+S
Sbjct: 1063 GSLGAEVS 1070
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.123    0.302 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 507,501
Number of extensions: 705
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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