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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1482
XP_006716142                                                           18    1482
XP_005250088                                                           18    1482
XP_006716141                                                           18    1482
XP_005250085                                                           18    1482
XP_006716140                                                           18    1482
XP_005250084                                                           18    1482
XP_005250083                                                           18    1482
XP_005250082                                                           18    1482
NP_705838                                                              16    5631
NP_001367                                                              16    7355
NP_060045                                                              16    7355
NP_001029025                                                           16    7355
NP_000958                                                              16    7355
XP_006726824                                                           16    7355
XP_006722329                                                           16    7355
XP_006721556                                                           16    7355
XP_005256626                                                           16    7355
XP_006710096                                                           16    7355
NP_001035364                                                           15    9605

>NP_733751
          Length = 4911

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLRTFSAIA 9
            GL TFSAIA
Sbjct: 1582 GLGTFSAIA 1590



>XP_006716142
          Length = 4927

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLRTFSAIA 9
            GL TFSAIA
Sbjct: 1599 GLGTFSAIA 1607



>XP_005250088
          Length = 4928

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLRTFSAIA 9
            GL TFSAIA
Sbjct: 1599 GLGTFSAIA 1607



>XP_006716141
          Length = 4959

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLRTFSAIA 9
            GL TFSAIA
Sbjct: 1599 GLGTFSAIA 1607



>XP_005250085
          Length = 4979

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLRTFSAIA 9
            GL TFSAIA
Sbjct: 1599 GLGTFSAIA 1607



>XP_006716140
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLRTFSAIA 9
            GL TFSAIA
Sbjct: 1599 GLGTFSAIA 1607



>XP_005250084
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLRTFSAIA 9
            GL TFSAIA
Sbjct: 1599 GLGTFSAIA 1607



>XP_005250083
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLRTFSAIA 9
            GL TFSAIA
Sbjct: 1598 GLGTFSAIA 1606



>XP_005250082
          Length = 4983

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLRTFSAIA 9
            GL TFSAIA
Sbjct: 1599 GLGTFSAIA 1607



>NP_705838
          Length = 4069

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    GLRTFSAI 8
            GLR+FS I
Sbjct: 3850 GLRSFSGI 3857



>NP_001367
          Length = 4646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    LRTFSAI 8
            LRTFS+I
Sbjct: 4158 LRTFSSI 4164



>NP_060045
          Length = 1419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GLRTFSAIA 9
           G+ TF A+A
Sbjct: 908 GMETFPAVA 916



>NP_001029025
          Length = 354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   GLRTFSAI 8
           GLRTF  +
Sbjct: 98  GLRTFKTV 105



>NP_000958
          Length = 403

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   GLRTFSAI 8
           GLRTF  +
Sbjct: 98  GLRTFKTV 105



>XP_006726824
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  GLRTFSA 7
          GLR FSA
Sbjct: 82 GLRAFSA 88



>XP_006722329
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  GLRTFSA 7
          GLR FSA
Sbjct: 82 GLRAFSA 88



>XP_006721556
          Length = 1357

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GLRTFSAIA 9
           G+ TF A+A
Sbjct: 846 GMETFPAVA 854



>XP_005256626
          Length = 1388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GLRTFSAIA 9
           G+ TF A+A
Sbjct: 877 GMETFPAVA 885



>XP_006710096
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  GLRTFSA 7
          GLR FSA
Sbjct: 82 GLRAFSA 88



>NP_001035364
          Length = 812

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GLRTFSAI 8
           GL+TF+ I
Sbjct: 185 GLKTFNCI 192



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.138    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 330,848
Number of extensions: 291
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)