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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001895                                                              19     665
NP_001091680                                                           19     665
NP_001091679                                                           19     665
XP_006713048                                                           19     665
XP_005264943                                                           19     665
XP_006713047                                                           18    1935
XP_006713046                                                           18    1935
NP_056508                                                              17    4312
XP_005258482                                                           16    5631
NP_036231                                                              16    5631
NP_001264352                                                           16    7355
XP_006724786                                                           16    7355
XP_005258069                                                           16    7355
XP_006710322                                                           16    7355
NP_742093                                                              15    9605
NP_001269238                                                           15    9605
NP_001269239                                                           15    9605
NP_473361                                                              15    9605
NP_695000                                                              15    9605
NP_001172035                                                           15    9605
NP_001273191                                                           15    9605
NP_689985                                                              15    9605
NP_002749                                                              15    9605
XP_005257574                                                           15    9605
XP_006722038                                                           15    9605
XP_005257572                                                           15    9605
XP_005257573                                                           15    9605
XP_005268826                                                           15    9605
XP_006719386                                                           15    9605
XP_005268825                                                           15    9605
XP_005268823                                                           15    9605
XP_005268824                                                           15    9605

>NP_001895
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  LMELDMAM 9
          LMELDMAM
Sbjct: 7  LMELDMAM 14



>NP_001091680
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  LMELDMAM 9
          LMELDMAM
Sbjct: 7  LMELDMAM 14



>NP_001091679
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  LMELDMAM 9
          LMELDMAM
Sbjct: 7  LMELDMAM 14



>XP_006713048
          Length = 720

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  LMELDMAM 9
          LMELDMAM
Sbjct: 7  LMELDMAM 14



>XP_005264943
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  LMELDMAM 9
          LMELDMAM
Sbjct: 7  LMELDMAM 14



>XP_006713047
          Length = 774

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MELDMAM 9
         MELDMAM
Sbjct: 1 MELDMAM 7



>XP_006713046
          Length = 774

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MELDMAM 9
         MELDMAM
Sbjct: 1 MELDMAM 7



>NP_056508
          Length = 942

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   MELDMAM 9
           MELDMA+
Sbjct: 484 MELDMAL 490



>XP_005258482
          Length = 512

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GLMELDMAM 9
           G +E+DMA+
Sbjct: 197 GALEVDMAL 205



>NP_036231
          Length = 510

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GLMELDMAM 9
           G +E+DMA+
Sbjct: 195 GALEVDMAL 203



>NP_001264352
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLMELDM 7
           GLM++DM
Sbjct: 103 GLMKMDM 109



>XP_006724786
          Length = 312

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLMELDM 7
           GLM++DM
Sbjct: 198 GLMKMDM 204



>XP_005258069
          Length = 476

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  GLMELDMA 8
          GL++LD+A
Sbjct: 39 GLLKLDLA 46



>XP_006710322
          Length = 657

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLMELDM 7
           GLM++DM
Sbjct: 267 GLMKMDM 273



>NP_742093
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GLMELD 6
           GLME+D
Sbjct: 480 GLMEMD 485



>NP_001269238
          Length = 528

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GLMELD 6
           GLME+D
Sbjct: 478 GLMEMD 483



>NP_001269239
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GLMELD 6
           GLME+D
Sbjct: 484 GLMEMD 489



>NP_473361
          Length = 528

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GLMELD 6
           GLME+D
Sbjct: 478 GLMEMD 483



>NP_695000
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GLMELD 6
           GLME+D
Sbjct: 142 GLMEID 147



>NP_001172035
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GLMELD 6
           GLME+D
Sbjct: 142 GLMEID 147



>NP_001273191
          Length = 266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LMELDMA 8
           LM++DMA
Sbjct: 220 LMQMDMA 226



>NP_689985
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LMELDMA 8
           LM++DMA
Sbjct: 220 LMQMDMA 226



>NP_002749
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LMELDMAM 9
           LM+LD++M
Sbjct: 97  LMDLDISM 104



>XP_005257574
          Length = 278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LMELDMAM 9
          LM+LD++M
Sbjct: 41 LMDLDISM 48



>XP_006722038
          Length = 278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LMELDMAM 9
          LM+LD++M
Sbjct: 41 LMDLDISM 48



>XP_005257572
          Length = 278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LMELDMAM 9
          LM+LD++M
Sbjct: 41 LMDLDISM 48



>XP_005257573
          Length = 278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LMELDMAM 9
          LM+LD++M
Sbjct: 41 LMDLDISM 48



>XP_005268826
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LMELDMA 8
           LM++DMA
Sbjct: 155 LMQMDMA 161



>XP_006719386
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LMELDMA 8
           LM++DMA
Sbjct: 220 LMQMDMA 226



>XP_005268825
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LMELDMA 8
           LM++DMA
Sbjct: 220 LMQMDMA 226



>XP_005268823
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LMELDMA 8
           LM++DMA
Sbjct: 220 LMQMDMA 226



>XP_005268824
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LMELDMA 8
           LM++DMA
Sbjct: 220 LMQMDMA 226



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.139    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 422,640
Number of extensions: 533
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)