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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              19     869
NP_001087241                                                           19     869
XP_005265799                                                           19     869
XP_005265798                                                           19     869
XP_005265797                                                           19     869
NP_443723                                                              16    7355
NP_203748                                                              16    7355
NP_071733                                                              16    7355
XP_005271772                                                           16    7355
XP_005274111                                                           16    7355
XP_005274110                                                           16    7355
XP_006717590                                                           16    7355

>NP_000213
          Length = 976

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GLIKSNAAM 9
           GLIKS+AAM
Sbjct: 610 GLIKSDAAM 618



>NP_001087241
          Length = 972

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GLIKSNAAM 9
           GLIKS+AAM
Sbjct: 606 GLIKSDAAM 614



>XP_005265799
          Length = 973

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GLIKSNAAM 9
           GLIKS+AAM
Sbjct: 607 GLIKSDAAM 615



>XP_005265798
          Length = 976

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GLIKSNAAM 9
           GLIKS+AAM
Sbjct: 611 GLIKSDAAM 619



>XP_005265797
          Length = 977

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GLIKSNAAM 9
           GLIKS+AAM
Sbjct: 611 GLIKSDAAM 619



>NP_443723
          Length = 1341

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GLIKSNAAM 9
           GL+K+N  M
Sbjct: 669 GLLKANCGM 677



>NP_203748
          Length = 883

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLIKSNA 7
           GL+KSN+
Sbjct: 463 GLVKSNS 469



>NP_071733
          Length = 449

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GLIKSNAAM 9
          GL+K NA++
Sbjct: 36 GLVKRNASV 44



>XP_005271772
          Length = 884

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLIKSNA 7
           GL+KSN+
Sbjct: 464 GLVKSNS 470



>XP_005274111
          Length = 114

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GLIKSNAAM 9
          GL+K NA++
Sbjct: 36 GLVKRNASV 44



>XP_005274110
          Length = 449

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GLIKSNAAM 9
          GL+K NA++
Sbjct: 36 GLVKRNASV 44



>XP_006717590
          Length = 1397

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GLIKSNAAM 9
           GL+K+N  M
Sbjct: 725 GLLKANCGM 733



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.123    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,990
Number of extensions: 274
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)