Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036284                                                              17    2528
XP_006710605                                                           17    2528
NP_689804                                                              17    3301
NP_001273173                                                           17    3301
NP_001273172                                                           17    3301
XP_006719326                                                           17    3301
XP_006719325                                                           17    3301
NP_000292                                                              17    4312
NP_068380                                                              16    5631
NP_001119593                                                           16    5631
NP_001119594                                                           16    5631
NP_005325                                                              16    5631
XP_005274721                                                           16    5631
XP_006724879                                                           16    5631
XP_006724878                                                           16    5631
XP_005257619                                                           16    5631
XP_006722070                                                           16    5631
XP_005257617                                                           16    5631
XP_005257618                                                           16    5631
XP_005257616                                                           16    5631
NP_060695                                                              16    7355
XP_005251560                                                           16    7355
NP_919047                                                              15    9605
NP_001269796                                                           15    9605
NP_001269800                                                           15    9605
NP_001269801                                                           15    9605
NP_001269799                                                           15    9605
NP_001121163                                                           15    9605
NP_057208                                                              15    9605
NP_001269797                                                           15    9605
NP_001269798                                                           15    9605
NP_000252                                                              15    9605
NP_002514                                                              15    9605
XP_005250598                                                           15    9605
NP_899234                                                              15    9605
>NP_036284
          Length = 353

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  GKVSKTLTL 9
          G VSKTLT+
Sbjct: 91 GPVSKTLTV 99
>XP_006710605
          Length = 353

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  GKVSKTLTL 9
          G VSKTLT+
Sbjct: 91 GPVSKTLTV 99
>NP_689804
          Length = 563

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           G+VSKTL L
Sbjct: 401 GEVSKTLDL 409
>NP_001273173
          Length = 484

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           G+VSKTL L
Sbjct: 322 GEVSKTLDL 330
>NP_001273172
          Length = 523

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           G+VSKTL L
Sbjct: 361 GEVSKTLDL 369
>XP_006719326
          Length = 539

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           G+VSKTL L
Sbjct: 401 GEVSKTLDL 409
>XP_006719325
          Length = 563

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           G+VSKTL L
Sbjct: 401 GEVSKTLDL 409
>NP_000292
          Length = 810

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GKVSKTLT 8
           GK+SKT++
Sbjct: 195 GKISKTMS 202
>NP_068380
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKVSKTLT 8
           G +SKTLT
Sbjct: 227 GVISKTLT 234
>NP_001119593
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKVSKTLT 8
           G +SKTLT
Sbjct: 227 GVISKTLT 234
>NP_001119594
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKVSKTLT 8
           G +SKTLT
Sbjct: 227 GVISKTLT 234
>NP_005325
          Length = 2035

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           G V+KT+TL
Sbjct: 655 GGVTKTITL 663
>XP_005274721
          Length = 2036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           G V+KT+TL
Sbjct: 655 GGVTKTITL 663
>XP_006724879
          Length = 2079

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           G V+KT+TL
Sbjct: 655 GGVTKTITL 663
>XP_006724878
          Length = 2080

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           G V+KT+TL
Sbjct: 655 GGVTKTITL 663
>XP_005257619
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKVSKTLT 8
           G +SKTLT
Sbjct: 227 GVISKTLT 234
>XP_006722070
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKVSKTLT 8
           G +SKTLT
Sbjct: 227 GVISKTLT 234
>XP_005257617
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKVSKTLT 8
           G +SKTLT
Sbjct: 227 GVISKTLT 234
>XP_005257618
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKVSKTLT 8
           G +SKTLT
Sbjct: 227 GVISKTLT 234
>XP_005257616
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKVSKTLT 8
           G +SKTLT
Sbjct: 227 GVISKTLT 234
>NP_060695
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           GK+ +TLT+
Sbjct: 474 GKLERTLTV 482
>XP_005251560
          Length = 462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GKVSKTLTL 9
           GK+ +TLT+
Sbjct: 423 GKLERTLTV 431
>NP_919047
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GKVSKTLTL 9
           GKVS+T  L
Sbjct: 363 GKVSRTCKL 371
>NP_001269796
          Length = 251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKVSKT 6
           GK+SKT
Sbjct: 97  GKISKT 102
>NP_001269800
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKVSKT 6
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_001269801
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKVSKT 6
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_001269799
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKVSKT 6
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_001121163
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKVSKT 6
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_057208
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKVSKT 6
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_001269797
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKVSKT 6
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_001269798
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKVSKT 6
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_000252
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VSKTLTL 9
           +SKTLT+
Sbjct: 459 ISKTLTI 465
>NP_002514
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GKVSKTL 7
           GK+ KTL
Sbjct: 238 GKIKKTL 244
>XP_005250598
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GKVSKTLTL 9
           GKVS+T  L
Sbjct: 300 GKVSRTCKL 308
>NP_899234
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKVSKT 6
           GK+SKT
Sbjct: 179 GKISKT 184
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.124    0.304 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 330,764
Number of extensions: 404
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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