Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
NP_057427                                                              17    3301
NP_056366                                                              17    4312
XP_005265092                                                           17    4312
XP_005265091                                                           17    4312
NP_000693                                                              16    5631
NP_001035373                                                           16    5631
XP_006723384                                                           16    5631
XP_005259205                                                           16    5631
XP_005259203                                                           16    5631
XP_006723383                                                           16    5631
NP_006412                                                              16    7355
NP_006411                                                              16    7355
XP_006723746                                                           16    7355
XP_005260309                                                           16    7355
XP_006716481                                                           16    7355
XP_005251193                                                           16    7355
XP_005251192                                                           16    7355
XP_005251191                                                           16    7355
NP_789791                                                              16    7355
NP_001191243                                                           15    9605
NP_000940                                                              15    9605
NP_001191247                                                           15    9605
NP_055177                                                              15    9605
NP_001191245                                                           15    9605
NP_932164                                                              15    9605
NP_001191246                                                           15    9605
NP_001191244                                                           15    9605
NP_001276065                                                           15    9605
NP_004778                                                              15    9605
NP_001276064                                                           15    9605
NP_001185734                                                           15    9605
NP_001185736                                                           15    9605
NP_001185735                                                           15    9605
NP_006763                                                              15    9605
NP_542386                                                              15    9605
NP_002902                                                              15    9605
NP_060052                                                              15    9605
NP_001185741                                                           15    9605
XP_006724836                                                           15    9605
XP_006724833                                                           15    9605
XP_006724832                                                           15    9605
XP_006724831                                                           15    9605
XP_006724830                                                           15    9605
XP_005262504                                                           15    9605
XP_005260072                                                           15    9605
XP_006714547                                                           15    9605
XP_005248271                                                           15    9605
XP_006714049                                                           15    9605
XP_005248268                                                           15    9605
XP_005245372                                                           15    9605
XP_006710230                                                           15    9605
>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GKENIASSK 9
            GKEN ASSK
Sbjct: 1467 GKENTASSK 1475
>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GKENIASSK 9
            GKEN ASSK
Sbjct: 1467 GKENTASSK 1475
>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GKENIASSK 9
            GKEN ASSK
Sbjct: 1467 GKENTASSK 1475
>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GKENIASSK 9
            GKEN ASSK
Sbjct: 1467 GKENTASSK 1475
>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GKENIASSK 9
            GKEN ASSK
Sbjct: 1467 GKENTASSK 1475
>NP_057427
          Length = 3114

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    GKENIASS 8
            GKEN+A S
Sbjct: 3028 GKENLAES 3035
>NP_056366
          Length = 629

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GKENIASSK 9
           G+ENIA  K
Sbjct: 256 GRENIARDK 264
>XP_005265092
          Length = 451

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GKENIASSK 9
          G+ENIA  K
Sbjct: 78 GRENIARDK 86
>XP_005265091
          Length = 566

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GKENIASSK 9
           G+ENIA  K
Sbjct: 193 GRENIARDK 201
>NP_000693
          Length = 1020

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GKENIASSK 9
           G+ENI+ SK
Sbjct: 434 GQENISVSK 442
>NP_001035373
          Length = 367

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GKENIA 6
           GKENIA
Sbjct: 125 GKENIA 130
>XP_006723384
          Length = 216

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GKENIA 6
           GKENIA
Sbjct: 128 GKENIA 133
>XP_005259205
          Length = 367

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GKENIA 6
           GKENIA
Sbjct: 125 GKENIA 130
>XP_005259203
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GKENIA 6
           GKENIA
Sbjct: 128 GKENIA 133
>XP_006723383
          Length = 418

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GKENIA 6
           GKENIA
Sbjct: 125 GKENIA 130
>NP_006412
          Length = 1849

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KENIASSK 9
           K+N+AS K
Sbjct: 898 KQNVASEK 905
>NP_006411
          Length = 1785

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KENIASSK 9
           K+N+AS K
Sbjct: 843 KQNVASEK 850
>XP_006723746
          Length = 1177

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KENIASSK 9
           K+N+AS K
Sbjct: 235 KQNVASEK 242
>XP_005260309
          Length = 1784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KENIASSK 9
           K+N+AS K
Sbjct: 842 KQNVASEK 849
>XP_006716481
          Length = 1649

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KENIASSK 9
           K+N+AS K
Sbjct: 698 KQNVASEK 705
>XP_005251193
          Length = 1800

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KENIASSK 9
           K+N+AS K
Sbjct: 898 KQNVASEK 905
>XP_005251192
          Length = 1843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KENIASSK 9
           K+N+AS K
Sbjct: 898 KQNVASEK 905
>XP_005251191
          Length = 1849

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KENIASSK 9
           K+N+AS K
Sbjct: 898 KQNVASEK 905
>NP_789791
          Length = 655

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GKENIASSK 9
           GK+NIA ++
Sbjct: 627 GKDNIAGTQ 635
>NP_001191243
          Length = 521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 KENIASS 8
         KEN+AS+
Sbjct: 2 KENVASA 8
>NP_000940
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 KENIASS 8
         KEN+AS+
Sbjct: 2 KENVASA 8
>NP_001191247
          Length = 268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 KENIASS 8
         KEN+AS+
Sbjct: 2 KENVASA 8
>NP_055177
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KENIAS 7
           KENIAS
Sbjct: 237 KENIAS 242
>NP_001191245
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 KENIASS 8
         KEN+AS+
Sbjct: 2 KENVASA 8
>NP_932164
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KENIAS 7
           KENIAS
Sbjct: 237 KENIAS 242
>NP_001191246
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 KENIASS 8
         KEN+AS+
Sbjct: 2 KENVASA 8
>NP_001191244
          Length = 349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 KENIASS 8
         KEN+AS+
Sbjct: 2 KENVASA 8
>NP_001276065
          Length = 1521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    GKENIAS 7
            GK N+AS
Sbjct: 1278 GKSNVAS 1284
>NP_004778
          Length = 1529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    GKENIAS 7
            GK N+AS
Sbjct: 1286 GKSNVAS 1292
>NP_001276064
          Length = 1525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    GKENIAS 7
            GK N+AS
Sbjct: 1282 GKSNVAS 1288
>NP_001185734
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GKENIAS 7
           GKE IAS
Sbjct: 107 GKEKIAS 113
>NP_001185736
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GKENIAS 7
           GKE IAS
Sbjct: 107 GKEKIAS 113
>NP_001185735
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GKENIAS 7
           GKE IAS
Sbjct: 107 GKEKIAS 113
>NP_006763
          Length = 1343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GKENIASS 8
           GKE +AS+
Sbjct: 443 GKEEVASA 450
>NP_542386
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GKENIAS 7
           GKE IAS
Sbjct: 107 GKEKIAS 113
>NP_002902
          Length = 1118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKENIASS 8
           G+E IASS
Sbjct: 753 GQEEIASS 760
>NP_060052
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKENIA 6
           GKEN+A
Sbjct: 223 GKENLA 228
>NP_001185741
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GKENIAS 7
           GKE IAS
Sbjct: 107 GKEKIAS 113
>XP_006724836
          Length = 1582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    GKENIASS 8
            G EN ASS
Sbjct: 1398 GSENYASS 1405
>XP_006724833
          Length = 1620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    GKENIASS 8
            G EN ASS
Sbjct: 1436 GSENYASS 1443
>XP_006724832
          Length = 1640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    GKENIASS 8
            G EN ASS
Sbjct: 1458 GSENYASS 1465
>XP_006724831
          Length = 1640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    GKENIASS 8
            G EN ASS
Sbjct: 1458 GSENYASS 1465
>XP_006724830
          Length = 1642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    GKENIASS 8
            G EN ASS
Sbjct: 1458 GSENYASS 1465
>XP_005262504
          Length = 1642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    GKENIASS 8
            G EN ASS
Sbjct: 1458 GSENYASS 1465
>XP_005260072
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKENIASS 8
           G+E IASS
Sbjct: 764 GQEEIASS 771
>XP_006714547
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 KENIASS 8
         KEN+AS+
Sbjct: 2 KENVASA 8
>XP_005248271
          Length = 1404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    GKENIAS 7
            GK N+AS
Sbjct: 1161 GKSNVAS 1167
>XP_006714049
          Length = 1509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    GKENIAS 7
            GK N+AS
Sbjct: 1266 GKSNVAS 1272
>XP_005248268
          Length = 1533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    GKENIAS 7
            GK N+AS
Sbjct: 1290 GKSNVAS 1296
>XP_005245372
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GKENIA 6
           GKEN+A
Sbjct: 223 GKENLA 228
>XP_006710230
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GKENIAS 7
           GKENI++
Sbjct: 128 GKENISA 134
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.284    0.107    0.250 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 431,258
Number of extensions: 951
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.7 bits)
S2: 28 (15.4 bits)

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