Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              19     665
NP_653080                                                              19     665
XP_005267292                                                           19     665
XP_005267291                                                           19     665
XP_005267290                                                           19     665
NP_005871                                                              16    5631
NP_073741                                                              16    5631
NP_002366                                                              16    5631
NP_001127836                                                           16    5631
NP_001120843                                                           16    5631
NP_001120842                                                           16    5631
NP_061189                                                              16    5631
XP_005265216                                                           16    5631
XP_005265214                                                           16    5631
XP_005265212                                                           16    5631
XP_006713225                                                           16    5631
XP_006713224                                                           16    5631
XP_006713223                                                           16    5631
XP_006713222                                                           16    5631
XP_005265204                                                           16    5631
XP_006713220                                                           16    5631
XP_006713219                                                           16    5631
XP_006713218                                                           16    5631
XP_005265196                                                           16    5631
XP_005265195                                                           16    5631
XP_006713216                                                           16    5631
XP_006713215                                                           16    5631
XP_006713214                                                           16    5631
XP_005265192                                                           16    5631
XP_005265191                                                           16    5631
XP_005265190                                                           16    5631
XP_006713212                                                           16    5631
XP_006713211                                                           16    5631
XP_006713210                                                           16    5631
XP_006713209                                                           16    5631
XP_006713208                                                           16    5631
XP_006713207                                                           16    5631
XP_006713206                                                           16    5631
XP_006713205                                                           16    5631
XP_006713204                                                           16    5631
XP_006713203                                                           16    5631
NP_065393                                                              16    7355
NP_997404                                                              16    7355
NP_060217                                                              16    7355
NP_065741                                                              16    7355
XP_006724984                                                           16    7355
XP_006724983                                                           16    7355
XP_006724982                                                           16    7355
XP_006724981                                                           16    7355
XP_006724980                                                           16    7355
XP_006724979                                                           16    7355
XP_006724978                                                           16    7355
XP_006724977                                                           16    7355
XP_006724976                                                           16    7355
XP_006721807                                                           16    7355
XP_005264491                                                           16    7355
NP_001180209                                                           15    9605
NP_001180207                                                           15    9605
NP_001180208                                                           15    9605
NP_000099                                                              15    9605
NP_003627                                                              15    9605
NP_001186791                                                           15    9605
NP_001276680                                                           15    9605
NP_001276679                                                           15    9605
NP_742128                                                              15    9605
NP_001186792                                                           15    9605
NP_001276681                                                           15    9605
NP_001186789                                                           15    9605
NP_001186790                                                           15    9605
XP_005274595                                                           15    9605
XP_005263571                                                           15    9605
XP_005263570                                                           15    9605
XP_005263569                                                           15    9605
XP_005263568                                                           15    9605
NP_849150                                                              15    9605
>NP_005091
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GKAEV SEK
Sbjct: 341 GKAEVASEK 349
>NP_653080
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GKAEV SEK
Sbjct: 243 GKAEVASEK 251
>XP_005267292
          Length = 1677

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GKAEV SEK
Sbjct: 236 GKAEVASEK 244
>XP_005267291
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GKAEV SEK
Sbjct: 243 GKAEVASEK 251
>XP_005267290
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GKAEV SEK
Sbjct: 341 GKAEVASEK 349
>NP_005871
          Length = 412

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           G+ EV++EK
Sbjct: 191 GEGEVINEK 199
>NP_073741
          Length = 912

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GKAEVVSEK 9
          G AEV SE+
Sbjct: 6  GSAEVASER 14
>NP_002366
          Length = 1152

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GK ++VS+K
Sbjct: 990 GKVQIVSKK 998
>NP_001127836
          Length = 1135

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GK ++VS+K
Sbjct: 990 GKVQIVSKK 998
>NP_001120843
          Length = 408

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKA +VSE+
Sbjct: 65 GKASLVSEE 73
>NP_001120842
          Length = 420

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKA +VSE+
Sbjct: 77 GKASLVSEE 85
>NP_061189
          Length = 454

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GKA +VSE+
Sbjct: 111 GKASLVSEE 119
>XP_005265216
          Length = 903

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GK ++VS+K
Sbjct: 741 GKVQIVSKK 749
>XP_005265214
          Length = 975

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GK ++VS+K
Sbjct: 813 GKVQIVSKK 821
>XP_005265212
          Length = 1129

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GK ++VS+K
Sbjct: 967 GKVQIVSKK 975
>XP_006713225
          Length = 1135

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GKAEVVSEK 9
           GK ++VS+K
Sbjct: 973 GKVQIVSKK 981
>XP_006713224
          Length = 1155

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 993  GKVQIVSKK 1001
>XP_006713223
          Length = 1178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 1016 GKVQIVSKK 1024
>XP_006713222
          Length = 1207

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 1045 GKVQIVSKK 1053
>XP_005265204
          Length = 2256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2094 GKVQIVSKK 2102
>XP_006713220
          Length = 2274

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2112 GKVQIVSKK 2120
>XP_006713219
          Length = 2280

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2118 GKVQIVSKK 2126
>XP_006713218
          Length = 2282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2120 GKVQIVSKK 2128
>XP_005265196
          Length = 2297

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2135 GKVQIVSKK 2143
>XP_005265195
          Length = 2297

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2135 GKVQIVSKK 2143
>XP_006713216
          Length = 2300

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2138 GKVQIVSKK 2146
>XP_006713215
          Length = 2309

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2147 GKVQIVSKK 2155
>XP_006713214
          Length = 2311

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2149 GKVQIVSKK 2157
>XP_005265192
          Length = 2314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2152 GKVQIVSKK 2160
>XP_005265191
          Length = 2314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2152 GKVQIVSKK 2160
>XP_005265190
          Length = 2314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2152 GKVQIVSKK 2160
>XP_006713212
          Length = 2320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2158 GKVQIVSKK 2166
>XP_006713211
          Length = 2323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2161 GKVQIVSKK 2169
>XP_006713210
          Length = 2326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2164 GKVQIVSKK 2172
>XP_006713209
          Length = 2326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2164 GKVQIVSKK 2172
>XP_006713208
          Length = 2335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2173 GKVQIVSKK 2181
>XP_006713207
          Length = 2335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2173 GKVQIVSKK 2181
>XP_006713206
          Length = 2337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2175 GKVQIVSKK 2183
>XP_006713205
          Length = 2340

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2178 GKVQIVSKK 2186
>XP_006713204
          Length = 2351

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2190 GKVQIVSKK 2198
>XP_006713203
          Length = 2352

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GKAEVVSEK 9
            GK ++VS+K
Sbjct: 2190 GKVQIVSKK 2198
>NP_065393
          Length = 1192

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KAEVVSEK 9
           KA++V+EK
Sbjct: 501 KAQIVTEK 508
>NP_997404
          Length = 986

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KAEVVSEK 9
           KA++V+EK
Sbjct: 295 KAQIVTEK 302
>NP_060217
          Length = 2542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GKAEVVS 7
            G+AEVVS
Sbjct: 1235 GRAEVVS 1241
>NP_065741
          Length = 2617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GKAEVVS 7
            G+AEVVS
Sbjct: 1235 GRAEVVS 1241
>XP_006724984
          Length = 517

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKAEV++ +
Sbjct: 20 GKAEVINAR 28
>XP_006724983
          Length = 525

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKAEV++ +
Sbjct: 68 GKAEVINAR 76
>XP_006724982
          Length = 538

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKAEV++ +
Sbjct: 68 GKAEVINAR 76
>XP_006724981
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKAEV++ +
Sbjct: 68 GKAEVINAR 76
>XP_006724980
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKAEV++ +
Sbjct: 68 GKAEVINAR 76
>XP_006724979
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKAEV++ +
Sbjct: 68 GKAEVINAR 76
>XP_006724978
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKAEV++ +
Sbjct: 68 GKAEVINAR 76
>XP_006724977
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKAEV++ +
Sbjct: 68 GKAEVINAR 76
>XP_006724976
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GKAEVVSEK 9
          GKAEV++ +
Sbjct: 68 GKAEVINAR 76
>XP_006721807
          Length = 742

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEVVSEK 9
           AE++SEK
Sbjct: 732 AEIISEK 738
>XP_005264491
          Length = 986

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KAEVVSEK 9
           KA++V+EK
Sbjct: 295 KAQIVTEK 302
>NP_001180209
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKAEVVSE 8
           G+A VVSE
Sbjct: 385 GQANVVSE 392
>NP_001180207
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKAEVVSE 8
           G+A VVSE
Sbjct: 474 GQANVVSE 481
>NP_001180208
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKAEVVSE 8
           G+A VVSE
Sbjct: 423 GQANVVSE 430
>NP_000099
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GKAEVVS 7
           GKAEV++
Sbjct: 299 GKAEVIT 305
>NP_003627
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GKAEVV 6
          GKAEVV
Sbjct: 93 GKAEVV 98
>NP_001186791
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GKAEVV 6
           GKAEVV
Sbjct: 126 GKAEVV 131
>NP_001276680
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GKAEVVS 7
           GKAEV++
Sbjct: 276 GKAEVIT 282
>NP_001276679
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GKAEVVS 7
           GKAEV++
Sbjct: 200 GKAEVIT 206
>NP_742128
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GKAEVV 6
          GKAEVV
Sbjct: 79 GKAEVV 84
>NP_001186792
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GKAEVV 6
          GKAEVV
Sbjct: 26 GKAEVV 31
>NP_001276681
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GKAEVVS 7
           GKAEV++
Sbjct: 251 GKAEVIT 257
>NP_001186789
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GKAEVV 6
          GKAEVV
Sbjct: 93 GKAEVV 98
>NP_001186790
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GKAEVV 6
          GKAEVV
Sbjct: 93 GKAEVV 98
>XP_005274595
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GKAEVVSE 8
           G+A VVSE
Sbjct: 474 GQANVVSE 481
>XP_005263571
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GKAEVV 6
          GKAEVV
Sbjct: 79 GKAEVV 84
>XP_005263570
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GKAEVV 6
          GKAEVV
Sbjct: 93 GKAEVV 98
>XP_005263569
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GKAEVV 6
          GKAEVV
Sbjct: 93 GKAEVV 98
>XP_005263568
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  GKAEVV 6
          GKAEVV
Sbjct: 93 GKAEVV 98
>NP_849150
          Length = 1445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    KAEVVSE 8
            KA+VVSE
Sbjct: 1093 KAQVVSE 1099
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.288    0.113    0.260 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 371,765
Number of extensions: 854
Number of successful extensions: 75
Number of sequences better than 10000.0: 75
Number of HSP's gapped: 75
Number of HSP's successfully gapped: 75
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.8 bits)
S2: 28 (15.4 bits)

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