Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           20     299
NP_858045                                                              20     299
NP_006525                                                              20     299
NP_001167559                                                           20     299
NP_001008395                                                           17    2528
NP_115560                                                              17    3301
XP_005271313                                                           17    3301
XP_005271311                                                           17    3301
XP_006711009                                                           17    3301
XP_005271309                                                           17    3301
XP_006711008                                                           17    3301
NP_000262                                                              16    7355
NP_542402                                                              16    7355
NP_065829                                                              16    7355
NP_997465                                                              16    7355
NP_849195                                                              16    7355
NP_006725                                                              16    7355
NP_001073996                                                           16    7355
XP_005259257                                                           16    7355
XP_005259256                                                           16    7355
XP_006722542                                                           16    7355
XP_005258336                                                           16    7355
XP_005258335                                                           16    7355
XP_005258334                                                           16    7355
XP_005267014                                                           16    7355
XP_006712602                                                           16    7355
NP_003361                                                              16    7355
NP_036474                                                              16    7355
NP_001035203                                                           15    9605
NP_074035                                                              15    9605
NP_001035202                                                           15    9605
NP_002465                                                              15    9605
NP_002464                                                              15    9605
NP_002968                                                              15    9605
NP_001273148                                                           15    9605
NP_001242941                                                           15    9605
NP_001139281                                                           15    9605
NP_705618                                                              15    9605
NP_005955                                                              15    9605
NP_001070654                                                           15    9605
NP_443139                                                              15    9605
NP_004289                                                              15    9605
NP_079005                                                              15    9605
NP_001265241                                                           15    9605
NP_001243024                                                           15    9605
XP_006723449                                                           15    9605
XP_005256710                                                           15    9605
XP_005256709                                                           15    9605
XP_005256708                                                           15    9605
XP_006720233                                                           15    9605
XP_005267806                                                           15    9605
XP_006720232                                                           15    9605
XP_006720231                                                           15    9605
XP_006719284                                                           15    9605
XP_006719283                                                           15    9605
XP_006719282                                                           15    9605
XP_005246814                                                           15    9605
NP_057421                                                              15    9605
>NP_001167558
          Length = 1423

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GIMEPMNSN 9
           G MEPMNSN
Sbjct: 928 GRMEPMNSN 936
>NP_858045
          Length = 1424

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GIMEPMNSN 9
           G MEPMNSN
Sbjct: 928 GRMEPMNSN 936
>NP_006525
          Length = 1420

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GIMEPMNSN 9
           G MEPMNSN
Sbjct: 928 GRMEPMNSN 936
>NP_001167559
          Length = 1415

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GIMEPMNSN 9
           G MEPMNSN
Sbjct: 923 GRMEPMNSN 931
>NP_001008395
          Length = 333

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IMEPMNSN 9
           I+EPMN N
Sbjct: 282 ILEPMNVN 289
>NP_115560
          Length = 1194

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GIMEPMNSN 9
           GIMEP + N
Sbjct: 494 GIMEPSSPN 502
>XP_005271313
          Length = 936

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GIMEPMNSN 9
           GIMEP + N
Sbjct: 236 GIMEPSSPN 244
>XP_005271311
          Length = 1051

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GIMEPMNSN 9
           GIMEP + N
Sbjct: 691 GIMEPSSPN 699
>XP_006711009
          Length = 1108

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GIMEPMNSN 9
           GIMEP + N
Sbjct: 691 GIMEPSSPN 699
>XP_005271309
          Length = 1228

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GIMEPMNSN 9
           GIMEP + N
Sbjct: 691 GIMEPSSPN 699
>XP_006711008
          Length = 1262

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GIMEPMNSN 9
           GIMEP + N
Sbjct: 691 GIMEPSSPN 699
>NP_000262
          Length = 1278

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MEPMNS 8
           MEPMN+
Sbjct: 217 MEPMNN 222
>NP_542402
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  MEPMNS 8
          MEP+NS
Sbjct: 55 MEPLNS 60
>NP_065829
          Length = 1907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GIMEPMN 7
           GI+EPM+
Sbjct: 785 GIIEPMD 791
>NP_997465
          Length = 538

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  GIMEPMNS 8
          G+M P NS
Sbjct: 84 GLMRPANS 91
>NP_849195
          Length = 802

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GIMEPMNSN 9
           G +EP++SN
Sbjct: 663 GGLEPLDSN 671
>NP_006725
          Length = 2446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GIMEPM 6
           GIMEP+
Sbjct: 454 GIMEPL 459
>NP_001073996
          Length = 2116

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  GIMEPMNSN 9
          G++ PM+ N
Sbjct: 54 GVLSPMHPN 62
>XP_005259257
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GIMEPMNS 8
           G+ME MN+
Sbjct: 262 GLMEEMNA 269
>XP_005259256
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GIMEPMNS 8
           G+ME MN+
Sbjct: 261 GLMEEMNA 268
>XP_006722542
          Length = 1273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MEPMNS 8
           MEPMN+
Sbjct: 217 MEPMNN 222
>XP_005258336
          Length = 1286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MEPMNS 8
           MEPMN+
Sbjct: 217 MEPMNN 222
>XP_005258335
          Length = 1295

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MEPMNS 8
           MEPMN+
Sbjct: 217 MEPMNN 222
>XP_005258334
          Length = 1303

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MEPMNS 8
           MEPMN+
Sbjct: 217 MEPMNN 222
>XP_005267014
          Length = 2446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GIMEPM 6
           GIMEP+
Sbjct: 454 GIMEPL 459
>XP_006712602
          Length = 2142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  GIMEPMNSN 9
          G++ PM+ N
Sbjct: 54 GVLSPMHPN 62
>NP_003361
          Length = 380

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GIMEPMNS 8
           G+ME MN+
Sbjct: 262 GLMEEMNA 269
>NP_036474
          Length = 260

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  IMEPMNSN 9
          +++P NSN
Sbjct: 55 LLDPQNSN 62
>NP_001035203
          Length = 1979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 603 MDPLNDN 609
>NP_074035
          Length = 1938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 596 MDPLNDN 602
>NP_001035202
          Length = 1945

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 603 MDPLNDN 609
>NP_002465
          Length = 1972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 596 MDPLNDN 602
>NP_002464
          Length = 1960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 589 MDPLNDN 595
>NP_002968
          Length = 1977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EPMNSN 9
            EPMNS+
Sbjct: 1133 EPMNSD 1138
>NP_001273148
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EPMNSN 9
           EPMNS+
Sbjct: 524 EPMNSS 529
>NP_001242941
          Length = 2007

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 606 MDPLNDN 612
>NP_001139281
          Length = 2036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 623 MDPLNDN 629
>NP_705618
          Length = 1391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   GIMEPMNSN 9
           G M P N N
Sbjct: 747 GFMRPENGN 755
>NP_005955
          Length = 1976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 596 MDPLNDN 602
>NP_001070654
          Length = 2003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 623 MDPLNDN 629
>NP_443139
          Length = 1078

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EPMNSN 9
            EPMNS+
Sbjct: 1012 EPMNSS 1017
>NP_004289
          Length = 1332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   GIMEPMNSN 9
           G M P N N
Sbjct: 688 GFMRPENGN 696
>NP_079005
          Length = 1995

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 615 MDPLNDN 621
>NP_001265241
          Length = 1327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   GIMEPMNSN 9
           G M P N N
Sbjct: 747 GFMRPENGN 755
>NP_001243024
          Length = 1985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 605 MDPLNDN 611
>XP_006723449
          Length = 2003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 623 MDPLNDN 629
>XP_005256710
          Length = 1986

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 606 MDPLNDN 612
>XP_005256709
          Length = 1997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 596 MDPLNDN 602
>XP_005256708
          Length = 2007

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MEPMNSN 9
           M+P+N N
Sbjct: 606 MDPLNDN 612
>XP_006720233
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GIMEPMN 7
           G+ME MN
Sbjct: 309 GLMEEMN 315
>XP_005267806
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GIMEPMN 7
           G+ME MN
Sbjct: 272 GLMEEMN 278
>XP_006720232
          Length = 438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GIMEPMN 7
           G+ME MN
Sbjct: 309 GLMEEMN 315
>XP_006720231
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GIMEPMN 7
           G+ME MN
Sbjct: 309 GLMEEMN 315
>XP_006719284
          Length = 676

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EPMNSN 9
           EPMNS+
Sbjct: 610 EPMNSS 615
>XP_006719283
          Length = 968

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EPMNSN 9
           EPMNS+
Sbjct: 902 EPMNSS 907
>XP_006719282
          Length = 968

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EPMNSN 9
           EPMNS+
Sbjct: 902 EPMNSS 907
>XP_005246814
          Length = 1988

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EPMNSN 9
            EPMNS+
Sbjct: 1144 EPMNSD 1149
>NP_057421
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GIMEPMN 7
           G+ME MN
Sbjct: 268 GLMEEMN 274
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.125    0.354 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 662,671
Number of extensions: 1342
Number of successful extensions: 58
Number of sequences better than 10000.0: 58
Number of HSP's gapped: 58
Number of HSP's successfully gapped: 58
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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