Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005350                                                              17    2528
NP_066553                                                              17    4312
NP_001001503                                                           17    4312
NP_000375                                                              16    5631
NP_065910                                                              16    5631
XP_005263222                                                           16    5631
XP_005263221                                                           16    5631
XP_005263220                                                           16    5631
XP_006714345                                                           16    5631
XP_005263219                                                           16    5631
NP_001122060                                                           16    7355
NP_056949                                                              16    7355
NP_001139508                                                           16    7355
NP_001139509                                                           16    7355
XP_005258390                                                           16    7355
XP_005258389                                                           16    7355
XP_005258386                                                           16    7355
XP_006722589                                                           16    7355
NP_056081                                                              15    9605
NP_001247420                                                           15    9605
XP_006723821                                                           15    9605
XP_006723820                                                           15    9605
XP_006723819                                                           15    9605
>NP_005350
          Length = 552

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           GIA AISLS
Sbjct: 477 GIAPAISLS 485
>NP_066553
          Length = 473

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  GIASAISLS 9
          G+AS +SLS
Sbjct: 27 GLASTVSLS 35
>NP_001001503
          Length = 108

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  GIASAISLS 9
          G+AS +SLS
Sbjct: 27 GLASTVSLS 35
>NP_000375
          Length = 4563

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GIASAISLS 9
            G+ASAI +S
Sbjct: 1874 GLASAIDMS 1882
>NP_065910
          Length = 1996

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    GIASAISLS 9
            G+ASA SLS
Sbjct: 1132 GLASASSLS 1140
>XP_005263222
          Length = 1820

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           G+ASA SLS
Sbjct: 956 GLASASSLS 964
>XP_005263221
          Length = 1820

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           G+ASA SLS
Sbjct: 956 GLASASSLS 964
>XP_005263220
          Length = 1820

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           G+ASA SLS
Sbjct: 956 GLASASSLS 964
>XP_006714345
          Length = 1961

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    GIASAISLS 9
            G+ASA SLS
Sbjct: 1097 GLASASSLS 1105
>XP_005263219
          Length = 1996

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    GIASAISLS 9
            G+ASA SLS
Sbjct: 1132 GLASASSLS 1140
>NP_001122060
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           GIA+ +SLS
Sbjct: 323 GIAAGLSLS 331
>NP_056949
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           GIA+ +SLS
Sbjct: 267 GIAAGLSLS 275
>NP_001139508
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           GIA+ +SLS
Sbjct: 267 GIAAGLSLS 275
>NP_001139509
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           GIA+ +SLS
Sbjct: 323 GIAAGLSLS 331
>XP_005258390
          Length = 257

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           GIA+ +SLS
Sbjct: 135 GIAAGLSLS 143
>XP_005258389
          Length = 257

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           GIA+ +SLS
Sbjct: 135 GIAAGLSLS 143
>XP_005258386
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           GIA+ +SLS
Sbjct: 323 GIAAGLSLS 331
>XP_006722589
          Length = 424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GIASAISLS 9
           GIA+ +SLS
Sbjct: 302 GIAAGLSLS 310
>NP_056081
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GIASAISLS 9
           G+A  ISLS
Sbjct: 311 GLAEGISLS 319
>NP_001247420
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GIASAISLS 9
           G+A  ISLS
Sbjct: 327 GLAEGISLS 335
>XP_006723821
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GIASAISLS 9
          G+A  ISLS
Sbjct: 64 GLAEGISLS 72
>XP_006723820
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GIASAISLS 9
          G+A  ISLS
Sbjct: 64 GLAEGISLS 72
>XP_006723819
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GIASAISLS 9
           G+A  ISLS
Sbjct: 344 GLAEGISLS 352
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.120    0.273 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 223,063
Number of extensions: 247
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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