Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_116321                                                              17    2528
XP_005258102                                                           17    2528
NP_001136099                                                           17    3301
NP_997304                                                              17    3301
NP_001136100                                                           17    3301
NP_060614                                                              17    3301
NP_037468                                                              17    3301
NP_066022                                                              16    5631
XP_006723369                                                           16    5631
NP_001008708                                                           16    7355
NP_001030025                                                           15    9605
NP_000319                                                              15    9605
NP_001892                                                              15    9605
NP_001189333                                                           15    9605
NP_001188306                                                           15    9605
NP_001173                                                              15    9605
NP_689829                                                              15    9605
NP_078966                                                              15    9605
NP_653326                                                              15    9605
NP_061152                                                              15    9605
NP_891847                                                              15    9605
NP_060681                                                              15    9605
XP_006724971                                                           15    9605
XP_006724970                                                           15    9605
XP_005272690                                                           15    9605
XP_006724601                                                           15    9605
XP_005254741                                                           15    9605
XP_005254740                                                           15    9605
XP_005254739                                                           15    9605
XP_006716112                                                           15    9605
XP_005250550                                                           15    9605
XP_005248316                                                           15    9605
XP_006714512                                                           15    9605
XP_006714511                                                           15    9605
XP_006714510                                                           15    9605
XP_006713671                                                           15    9605
XP_006710801                                                           15    9605
NP_079232                                                              15    9605
>NP_116321
          Length = 637

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   HRTTSLIS 9
           H TTSLIS
Sbjct: 204 HSTTSLIS 211
>XP_005258102
          Length = 607

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   HRTTSLIS 9
           H TTSLIS
Sbjct: 174 HSTTSLIS 181
>NP_001136099
          Length = 437

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GHRTTSLI 8
           GHR TS++
Sbjct: 403 GHRDTSMV 410
>NP_997304
          Length = 694

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   HRTTSLI 8
           H+TTSLI
Sbjct: 370 HKTTSLI 376
>NP_001136100
          Length = 454

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GHRTTSLI 8
           GHR TS++
Sbjct: 381 GHRDTSMV 388
>NP_060614
          Length = 476

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GHRTTSLI 8
           GHR TS++
Sbjct: 403 GHRDTSMV 410
>NP_037468
          Length = 476

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GHRTTSLI 8
           GHR TS++
Sbjct: 403 GHRETSMV 410
>NP_066022
          Length = 2564

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HRTTSLIS 9
           HRT  LIS
Sbjct: 326 HRTVGLIS 333
>XP_006723369
          Length = 2394

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HRTTSLIS 9
           HRT  LIS
Sbjct: 326 HRTVGLIS 333
>NP_001008708
          Length = 184

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  GHRTTSLI 8
          G+RTT++I
Sbjct: 86 GYRTTTVI 93
>NP_001030025
          Length = 1152

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HRTTSLIS 9
           HRT  L+S
Sbjct: 232 HRTPQLVS 239
>NP_000319
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HRTTSLIS 9
           HRT  L+S
Sbjct: 232 HRTPQLVS 239
>NP_001892
          Length = 349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HRTTSL 7
           HRTT+L
Sbjct: 296 HRTTTL 301
>NP_001189333
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   GHRTTSLIS 9
           G  TTSLIS
Sbjct: 246 GAPTTSLIS 254
>NP_001188306
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   GHRTTSLIS 9
           G  TTSLIS
Sbjct: 191 GAPTTSLIS 199
>NP_001173
          Length = 539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   GHRTTSLIS 9
           G  TTSLIS
Sbjct: 219 GAPTTSLIS 227
>NP_689829
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   GHRTTSLI 8
           GH T SLI
Sbjct: 320 GHDTISLI 327
>NP_078966
          Length = 14507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HRTTSLIS 9
            H T+SL+S
Sbjct: 6243 HSTSSLVS 6250
>NP_653326
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GHRTTSLIS 9
           G RT+ LIS
Sbjct: 248 GDRTSDLIS 256
>NP_061152
          Length = 1858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    GHRTTS 6
            GH TTS
Sbjct: 1730 GHHTTS 1735
>NP_891847
          Length = 1858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    GHRTTS 6
            GH TTS
Sbjct: 1730 GHHTTS 1735
>NP_060681
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HRTTSLI 8
           H+T+SLI
Sbjct: 456 HKTSSLI 462
>XP_006724971
          Length = 138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HRTTSL 7
           H+TTSL
Sbjct: 119 HKTTSL 124
>XP_006724970
          Length = 138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HRTTSL 7
           H+TTSL
Sbjct: 119 HKTTSL 124
>XP_005272690
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HRTTSLIS 9
           HRT  L+S
Sbjct: 232 HRTPQLVS 239
>XP_006724601
          Length = 1020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HRTTSLIS 9
           HRT  L+S
Sbjct: 232 HRTPQLVS 239
>XP_005254741
          Length = 438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  GHRTTSLI 8
          GH TTS I
Sbjct: 24 GHFTTSTI 31
>XP_005254740
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   GHRTTSLI 8
           GH TTS I
Sbjct: 97  GHFTTSTI 104
>XP_005254739
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   GHRTTSLI 8
           GH TTS I
Sbjct: 97  GHFTTSTI 104
>XP_006716112
          Length = 1816

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    GHRTTS 6
            GH TTS
Sbjct: 1688 GHHTTS 1693
>XP_005250550
          Length = 1858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    GHRTTS 6
            GH TTS
Sbjct: 1730 GHHTTS 1735
>XP_005248316
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GHRTTSLIS 9
           G RT+ LIS
Sbjct: 257 GDRTSDLIS 265
>XP_006714512
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GHRTTSLIS 9
           G RT+ LIS
Sbjct: 265 GDRTSDLIS 273
>XP_006714511
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GHRTTSLIS 9
           G RT+ LIS
Sbjct: 265 GDRTSDLIS 273
>XP_006714510
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GHRTTSLIS 9
           G RT+ LIS
Sbjct: 265 GDRTSDLIS 273
>XP_006713671
          Length = 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   GHRTTSLI 8
           GH T SLI
Sbjct: 173 GHDTISLI 180
>XP_006710801
          Length = 647

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HRTTSLI 8
           H+T+SLI
Sbjct: 425 HKTSSLI 431
>NP_079232
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   GHRTTSLI 8
           GH TTS I
Sbjct: 229 GHFTTSTI 236
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.124    0.342 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 488,053
Number of extensions: 532
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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