Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              22     103
NP_055144                                                              19     869
NP_065768                                                              17    2528
XP_006724654                                                           17    2528
XP_006724653                                                           17    2528
NP_002537                                                              17    3301
NP_060139                                                              17    4312
NP_000220                                                              17    4312
NP_848537                                                              16    5631
NP_113659                                                              16    5631
NP_006784                                                              16    5631
NP_054817                                                              16    5631
NP_001129711                                                           16    5631
NP_942581                                                              16    5631
NP_060200                                                              16    5631
XP_005276776                                                           16    5631
XP_005262564                                                           16    5631
XP_006718768                                                           16    5631
XP_006718767                                                           16    5631
XP_006718766                                                           16    5631
XP_005272185                                                           16    5631
XP_006713275                                                           16    5631
XP_005265305                                                           16    5631
XP_006710324                                                           16    5631
NP_001231612                                                           16    7355
NP_000912                                                              16    7355
NP_004887                                                              16    7355
NP_001030337                                                           16    7355
NP_062823                                                              16    7355
NP_940967                                                              16    7355
XP_006723348                                                           16    7355
XP_006723347                                                           16    7355
XP_006719150                                                           16    7355
XP_006719149                                                           16    7355
NP_009011                                                              16    7355
NP_085130                                                              15    9605
NP_116216                                                              15    9605
NP_001191006                                                           15    9605
NP_004779                                                              15    9605
NP_056140                                                              15    9605
NP_536354                                                              15    9605
NP_940852                                                              15    9605
XP_005262137                                                           15    9605
XP_005266053                                                           15    9605
XP_005266052                                                           15    9605
XP_005266051                                                           15    9605
XP_006712185                                                           15    9605
XP_005264680                                                           15    9605
XP_006712184                                                           15    9605
>NP_004295
          Length = 1620

 Score = 21.9 bits (45), Expect =   103
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GHMNMAFSQ 9
            GH+NMAFSQ
Sbjct: 1474 GHVNMAFSQ 1482
>NP_055144
          Length = 1401

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    GHMNMAFSQ 9
            GH+N AF Q
Sbjct: 1275 GHLNQAFQQ 1283
>NP_065768
          Length = 1493

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GHMNMAFSQ 9
           GH N  FSQ
Sbjct: 338 GHQNCEFSQ 346
>XP_006724654
          Length = 1335

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GHMNMAFSQ 9
           GH N  FSQ
Sbjct: 180 GHQNCEFSQ 188
>XP_006724653
          Length = 1377

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GHMNMAFSQ 9
           GH N  FSQ
Sbjct: 222 GHQNCEFSQ 230
>NP_002537
          Length = 401

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GHMNMAFSQ 9
           GH N+ F Q
Sbjct: 286 GHANLTFEQ 294
>NP_060139
          Length = 1250

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GHMNMAFSQ 9
           GH  + FSQ
Sbjct: 476 GHQTLVFSQ 484
>NP_000220
          Length = 440

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HMNMAFS 8
           H+NM FS
Sbjct: 401 HLNMVFS 407
>NP_848537
          Length = 667

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HMNMAFS 8
           H+N+AFS
Sbjct: 616 HINVAFS 622
>NP_113659
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HMNMAFS 8
           H+N+AFS
Sbjct: 612 HINVAFS 618
>NP_006784
          Length = 256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GHMNMA 6
           GHMN+A
Sbjct: 154 GHMNIA 159
>NP_054817
          Length = 238

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GHMNMA 6
           GHMN+A
Sbjct: 136 GHMNIA 141
>NP_001129711
          Length = 595

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GHMNMAFSQ 9
           GH + +FSQ
Sbjct: 504 GHCSQSFSQ 512
>NP_942581
          Length = 776

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HMNMAFSQ 9
           HM +AFS+
Sbjct: 642 HMKLAFSK 649
>NP_060200
          Length = 776

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HMNMAFSQ 9
           HM +AFS+
Sbjct: 642 HMKLAFSK 649
>XP_005276776
          Length = 991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GHMNMA 6
           GH+NMA
Sbjct: 270 GHINMA 275
>XP_005262564
          Length = 991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GHMNMA 6
           GH+NMA
Sbjct: 270 GHINMA 275
>XP_006718768
          Length = 483

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HMNMAFS 8
           H+N+AFS
Sbjct: 432 HINVAFS 438
>XP_006718767
          Length = 616

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HMNMAFS 8
           H+N+AFS
Sbjct: 565 HINVAFS 571
>XP_006718766
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HMNMAFS 8
           H+N+AFS
Sbjct: 612 HINVAFS 618
>XP_005272185
          Length = 595

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GHMNMAFSQ 9
           GH + +FSQ
Sbjct: 504 GHCSQSFSQ 512
>XP_006713275
          Length = 776

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HMNMAFSQ 9
           HM +AFS+
Sbjct: 642 HMKLAFSK 649
>XP_005265305
          Length = 776

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HMNMAFSQ 9
           HM +AFS+
Sbjct: 642 HMKLAFSK 649
>XP_006710324
          Length = 943

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GHMNMA 6
           GH+NMA
Sbjct: 222 GHINMA 227
>NP_001231612
          Length = 819

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GHMNMAFSQ 9
           GHM   FS+
Sbjct: 475 GHMGYVFSK 483
>NP_000912
          Length = 1141

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GHMNMAFSQ 9
           GHM   FS+
Sbjct: 797 GHMGYVFSK 805
>NP_004887
          Length = 327

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GHMNMAFSQ 9
          G +N+AF Q
Sbjct: 50 GKVNLAFKQ 58
>NP_001030337
          Length = 251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GHMNMAFSQ 9
          G +N+AF Q
Sbjct: 50 GKVNLAFKQ 58
>NP_062823
          Length = 523

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GHMNMAFSQ 9
           GH+ +AF Q
Sbjct: 232 GHLALAFLQ 240
>NP_940967
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HMNMAFS 8
           H+N+AF+
Sbjct: 209 HLNLAFN 215
>XP_006723348
          Length = 394

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GHMNMAFSQ 9
           GH+ +AF Q
Sbjct: 103 GHLALAFLQ 111
>XP_006723347
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GHMNMAFSQ 9
           GH+ +AF Q
Sbjct: 232 GHLALAFLQ 240
>XP_006719150
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GHMNMAFSQ 9
           GHM   FS+
Sbjct: 479 GHMGYVFSK 487
>XP_006719149
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GHMNMAFSQ 9
           GHM   FS+
Sbjct: 797 GHMGYVFSK 805
>NP_009011
          Length = 80

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GHMNMAFSQ 9
          G+MN+A  Q
Sbjct: 39 GYMNIALEQ 47
>NP_085130
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HMNMAFSQ 9
           H N AF+Q
Sbjct: 263 HRNAAFNQ 270
>NP_116216
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   HMNMAFSQ 9
           H N++FS+
Sbjct: 294 HSNLSFSE 301
>NP_001191006
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GHMNMAFS 8
           G+M MAFS
Sbjct: 958 GNMIMAFS 965
>NP_004779
          Length = 1073

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GHMNMAFS 8
           G+M MAFS
Sbjct: 965 GNMIMAFS 972
>NP_056140
          Length = 2266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    GHMNMAFS 8
            GH N+A++
Sbjct: 2154 GHANIAYT 2161
>NP_536354
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  HMNMAFS 8
          H+N AFS
Sbjct: 80 HLNPAFS 86
>NP_940852
          Length = 268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1  GHMNMAFS 8
          GH+ M F+
Sbjct: 59 GHLQMGFA 66
>XP_005262137
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1  GHMNMAFS 8
          GH+ M F+
Sbjct: 59 GHLQMGFA 66
>XP_005266053
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HMNMAFSQ 9
           H N AF+Q
Sbjct: 263 HRNAAFNQ 270
>XP_005266052
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HMNMAFSQ 9
           H N AF+Q
Sbjct: 263 HRNAAFNQ 270
>XP_005266051
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HMNMAFSQ 9
           H N AF+Q
Sbjct: 263 HRNAAFNQ 270
>XP_006712185
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   HMNMAFSQ 9
           H N++FS+
Sbjct: 294 HSNLSFSE 301
>XP_005264680
          Length = 349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   HMNMAFSQ 9
           H N++FS+
Sbjct: 294 HSNLSFSE 301
>XP_006712184
          Length = 382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   HMNMAFSQ 9
           H N++FS+
Sbjct: 294 HSNLSFSE 301
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.124    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 420,937
Number of extensions: 809
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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