Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001180350                                                           20     509
NP_775756                                                              20     509
NP_722576                                                              20     509
XP_006723760                                                           20     509
NP_005777                                                              18    1135
XP_005266698                                                           18    1135
XP_005266697                                                           18    1135
NP_001135780                                                           18    1135
NP_690605                                                              17    2528
NP_068769                                                              17    2528
NP_065861                                                              17    3301
NP_001128523                                                           17    3301
NP_065991                                                              17    3301
NP_061836                                                              17    3301
NP_387505                                                              17    3301
XP_005261051                                                           17    3301
XP_005267973                                                           17    3301
NP_001116236                                                           16    5631
NP_000300                                                              16    5631
XP_005257719                                                           16    5631
XP_006711467                                                           16    5631
NP_001009894                                                           16    5631
NP_001124481                                                           16    7355
NP_001124480                                                           16    7355
NP_002826                                                              16    7355
NP_002218                                                              16    7355
NP_001275918                                                           16    7355
NP_001159451                                                           16    7355
NP_065191                                                              16    7355
NP_001275880                                                           16    7355
NP_057425                                                              16    7355
NP_056098                                                              16    7355
NP_001276091                                                           16    7355
NP_001034656                                                           16    7355
XP_006721364                                                           16    7355
XP_006717953                                                           16    7355
XP_006717952                                                           16    7355
XP_006717951                                                           16    7355
XP_006717950                                                           16    7355
XP_006717949                                                           16    7355
XP_006717948                                                           16    7355
XP_006716137                                                           16    7355
XP_005250575                                                           16    7355
XP_006716136                                                           16    7355
XP_006713783                                                           16    7355
XP_005247745                                                           16    7355
XP_006713782                                                           16    7355
XP_005264497                                                           16    7355
XP_005270898                                                           16    7355
XP_006710687                                                           16    7355
NP_006497                                                              16    7355
NP_001276092                                                           16    7355
NP_115645                                                              16    7355
NP_001265                                                              15    9605
NP_001231775                                                           15    9605
NP_001107593                                                           15    9605
NP_001107594                                                           15    9605
NP_653178                                                              15    9605
NP_848018                                                              15    9605
NP_695014                                                              15    9605
XP_005259473                                                           15    9605
XP_005259472                                                           15    9605
XP_005259470                                                           15    9605
XP_005259471                                                           15    9605
XP_006723567                                                           15    9605
XP_006718822                                                           15    9605
NP_777568                                                              15    9605
>NP_001180350
          Length = 1031

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GHFLK+ SS
Sbjct: 400 GHFLKVTSS 408
>NP_775756
          Length = 1034

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GHFLK+ SS
Sbjct: 403 GHFLKVTSS 411
>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GHFLK ESS
Sbjct: 161 GHFLKNESS 169
>XP_006723760
          Length = 1038

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GHFLK+ SS
Sbjct: 407 GHFLKVTSS 415
>NP_005777
          Length = 1032

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GHFLK+ +S
Sbjct: 394 GHFLKVTTS 402
>XP_005266698
          Length = 1032

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GHFLK+ +S
Sbjct: 394 GHFLKVTTS 402
>XP_005266697
          Length = 1077

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GHFLK+ +S
Sbjct: 439 GHFLKVTTS 447
>NP_001135780
          Length = 2156

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GH+L IESS
Sbjct: 909 GHYLYIESS 917
>NP_690605
          Length = 297

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   HFLKIES 8
           HFLK+ES
Sbjct: 145 HFLKMES 151
>NP_068769
          Length = 297

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   HFLKIES 8
           HFLK+ES
Sbjct: 145 HFLKMES 151
>NP_065861
          Length = 509

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GHFLKIES 8
          G FLK+ES
Sbjct: 14 GRFLKLES 21
>NP_001128523
          Length = 738

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GHFLKI 6
           GHFLK+
Sbjct: 633 GHFLKV 638
>NP_065991
          Length = 765

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GHFLKI 6
           GHFLK+
Sbjct: 633 GHFLKV 638
>NP_061836
          Length = 2320

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    FLKIESS 9
            FLKIESS
Sbjct: 1750 FLKIESS 1756
>NP_387505
          Length = 2269

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    FLKIESS 9
            FLKIESS
Sbjct: 1750 FLKIESS 1756
>XP_005261051
          Length = 2242

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    FLKIESS 9
            FLKIESS
Sbjct: 1672 FLKIESS 1678
>XP_005267973
          Length = 537

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GHFLKIES 8
          G FLK+ES
Sbjct: 42 GRFLKLES 49
>NP_001116236
          Length = 477

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GH+ K+ES+
Sbjct: 425 GHWQKLESA 433
>NP_000300
          Length = 477

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GH+ K+ES+
Sbjct: 425 GHWQKLESA 433
>XP_005257719
          Length = 302

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  GHFLKIES 8
          GHF  +ES
Sbjct: 13 GHFCSLES 20
>XP_006711467
          Length = 482

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GH+ K+ES+
Sbjct: 430 GHWQKLESA 438
>NP_001009894
          Length = 325

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HFLKIES 8
           HFLKI++
Sbjct: 306 HFLKIDN 312
>NP_001124481
          Length = 650

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GH +KI+S+
Sbjct: 329 GHAIKIKSA 337
>NP_001124480
          Length = 661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GH +KI+S+
Sbjct: 340 GHAIKIKSA 348
>NP_002826
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GH +KI+S+
Sbjct: 459 GHAIKIKSA 467
>NP_002218
          Length = 1154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GHFLKIE 7
           GHF K+E
Sbjct: 884 GHFGKVE 890
>NP_001275918
          Length = 2286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GHFLKIESS 9
            GH LK ESS
Sbjct: 1397 GHQLKGESS 1405
>NP_001159451
          Length = 1994

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GHFLKIESS 9
            GH LK ESS
Sbjct: 1105 GHQLKGESS 1113
>NP_065191
          Length = 858

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  GHFLKIES 8
          G +LK+ES
Sbjct: 8  GSYLKVES 15
>NP_001275880
          Length = 882

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  GHFLKIES 8
          G +LK+ES
Sbjct: 8  GSYLKVES 15
>NP_057425
          Length = 2302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GHFLKIESS 9
            GH LK ESS
Sbjct: 1413 GHQLKGESS 1421
>NP_056098
          Length = 675

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HFLKIESS 9
           HFLKI  S
Sbjct: 328 HFLKINVS 335
>NP_001276091
          Length = 874

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HFLKIES 8
           HFL IES
Sbjct: 334 HFLGIES 340
>NP_001034656
          Length = 263

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  HFLKIE 7
          HFLKI+
Sbjct: 68 HFLKID 73
>XP_006721364
          Length = 831

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HFLKIES 8
           HFL IES
Sbjct: 334 HFLGIES 340
>XP_006717953
          Length = 2008

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GHFLKIESS 9
            GH LK ESS
Sbjct: 1105 GHQLKGESS 1113
>XP_006717952
          Length = 2300

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GHFLKIESS 9
            GH LK ESS
Sbjct: 1397 GHQLKGESS 1405
>XP_006717951
          Length = 2315

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GHFLKIESS 9
            GH LK ESS
Sbjct: 1413 GHQLKGESS 1421
>XP_006717950
          Length = 2316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GHFLKIESS 9
            GH LK ESS
Sbjct: 1413 GHQLKGESS 1421
>XP_006717949
          Length = 2316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GHFLKIESS 9
            GH LK ESS
Sbjct: 1413 GHQLKGESS 1421
>XP_006717948
          Length = 2316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GHFLKIESS 9
            GH LK ESS
Sbjct: 1413 GHQLKGESS 1421
>XP_006716137
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GH +KI+S+
Sbjct: 344 GHAIKIKSA 352
>XP_005250575
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GH +KI+S+
Sbjct: 344 GHAIKIKSA 352
>XP_006716136
          Length = 748

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GHFLKIESS 9
           GH +KI+S+
Sbjct: 427 GHAIKIKSA 435
>XP_006713783
          Length = 633

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   GHFLKI 6
           GH+LK+
Sbjct: 194 GHYLKV 199
>XP_005247745
          Length = 850

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   GHFLKI 6
           GH+LK+
Sbjct: 415 GHYLKV 420
>XP_006713782
          Length = 854

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   GHFLKI 6
           GH+LK+
Sbjct: 415 GHYLKV 420
>XP_005264497
          Length = 730

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  GHFLKIES 8
          G +LK+ES
Sbjct: 8  GSYLKVES 15
>XP_005270898
          Length = 1154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GHFLKIE 7
           GHF K+E
Sbjct: 884 GHFGKVE 890
>XP_006710687
          Length = 1154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GHFLKIE 7
           GHF K+E
Sbjct: 884 GHFGKVE 890
>NP_006497
          Length = 849

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   GHFLKI 6
           GH+LK+
Sbjct: 415 GHYLKV 420
>NP_001276092
          Length = 809

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HFLKIES 8
           HFL IES
Sbjct: 269 HFLGIES 275
>NP_115645
          Length = 874

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HFLKIES 8
           HFL IES
Sbjct: 334 HFLGIES 340
>NP_001265
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HFLKIE 7
           HFLKI+
Sbjct: 454 HFLKIK 459
>NP_001231775
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HFLKIE 7
           HFLKI+
Sbjct: 420 HFLKIK 425
>NP_001107593
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HFLKIE 7
           HFLKI+
Sbjct: 454 HFLKIK 459
>NP_001107594
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HFLKIE 7
           HFLKI+
Sbjct: 454 HFLKIK 459
>NP_653178
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   HFLKIESS 9
           HFLK++++
Sbjct: 225 HFLKLKNN 232
>NP_848018
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HFLKIESS 9
           HF K ESS
Sbjct: 467 HFSKSESS 474
>NP_695014
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HFLKIES 8
           HFL +ES
Sbjct: 296 HFLPLES 302
>XP_005259473
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  HFLKIESS 9
          HFLK++++
Sbjct: 34 HFLKLKNN 41
>XP_005259472
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   HFLKIESS 9
           HFLK++++
Sbjct: 262 HFLKLKNN 269
>XP_005259470
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   HFLKIESS 9
           HFLK++++
Sbjct: 262 HFLKLKNN 269
>XP_005259471
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   HFLKIESS 9
           HFLK++++
Sbjct: 262 HFLKLKNN 269
>XP_006723567
          Length = 720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   HFLKIESS 9
           HFLK++++
Sbjct: 275 HFLKLKNN 282
>XP_006718822
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HFLKIE 7
           HFLKI+
Sbjct: 454 HFLKIK 459
>NP_777568
          Length = 306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HFLKIESS 9
           HF K ESS
Sbjct: 291 HFSKSESS 298
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.136    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 506,665
Number of extensions: 715
Number of successful extensions: 67
Number of sequences better than 10000.0: 67
Number of HSP's gapped: 67
Number of HSP's successfully gapped: 67
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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