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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              22      79
NP_060132                                                              18    1935
NP_001170781                                                           18    1935
NP_536354                                                              18    1935
XP_006717022                                                           18    1935
NP_001170782                                                           18    1935
NP_060501                                                              17    2528
XP_005264894                                                           17    2528
NP_057413                                                              17    4312
NP_000220                                                              17    4312
XP_006713267                                                           17    4312
XP_005265278                                                           17    4312
XP_006713266                                                           17    4312
NP_848537                                                              16    5631
NP_113659                                                              16    5631
NP_001035351                                                           16    5631
NP_006784                                                              16    5631
NP_054817                                                              16    5631
XP_005276776                                                           16    5631
XP_005262564                                                           16    5631
XP_006718768                                                           16    5631
XP_006718767                                                           16    5631
XP_006718766                                                           16    5631
XP_005262842                                                           16    5631
XP_006710324                                                           16    5631
NP_942581                                                              16    7355
NP_940967                                                              16    7355
NP_060200                                                              16    7355
XP_006713275                                                           16    7355
XP_005265305                                                           16    7355
NP_001191006                                                           15    9605
NP_004779                                                              15    9605
NP_056140                                                              15    9605
NP_940852                                                              15    9605
XP_005262137                                                           15    9605

>NP_004295
          Length = 1620

 Score = 22.3 bits (46), Expect =    79
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    GGHMNMAFS 9
            GGH+NMAFS
Sbjct: 1473 GGHVNMAFS 1481



>NP_060132
          Length = 2022

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GGHMNMAFS 9
            GG++N AFS
Sbjct: 1450 GGYVNWAFS 1458



>NP_001170781
          Length = 2017

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GGHMNMAFS 9
            GG++N AFS
Sbjct: 1445 GGYVNWAFS 1453



>NP_536354
          Length = 301

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GGHMNMAFS 9
          G H+N AFS
Sbjct: 78 GAHLNPAFS 86



>XP_006717022
          Length = 1054

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GGHMNMAFS 9
           GG++N AFS
Sbjct: 482 GGYVNWAFS 490



>NP_001170782
          Length = 2017

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GGHMNMAFS 9
            GG++N AFS
Sbjct: 1445 GGYVNWAFS 1453



>NP_060501
          Length = 1151

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GGHMNMAFS 9
           GGH + AFS
Sbjct: 685 GGHRSWAFS 693



>XP_005264894
          Length = 1070

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GGHMNMAFS 9
           GGH + AFS
Sbjct: 604 GGHRSWAFS 612



>NP_057413
          Length = 866

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GGHMNMA 7
           GGH N+A
Sbjct: 652 GGHSNLA 658



>NP_000220
          Length = 440

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   HMNMAFS 9
           H+NM FS
Sbjct: 401 HLNMVFS 407



>XP_006713267
          Length = 866

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GGHMNMA 7
           GGH N+A
Sbjct: 652 GGHSNLA 658



>XP_005265278
          Length = 866

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GGHMNMA 7
           GGH N+A
Sbjct: 652 GGHSNLA 658



>XP_006713266
          Length = 866

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GGHMNMA 7
           GGH N+A
Sbjct: 652 GGHSNLA 658



>NP_848537
          Length = 667

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HMNMAFS 9
           H+N+AFS
Sbjct: 616 HINVAFS 622



>NP_113659
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HMNMAFS 9
           H+N+AFS
Sbjct: 612 HINVAFS 618



>NP_001035351
          Length = 783

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GGHMNMAFS 9
           GGH + A+S
Sbjct: 324 GGHYSSAYS 332



>NP_006784
          Length = 256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GHMNMA 7
           GHMN+A
Sbjct: 154 GHMNIA 159



>NP_054817
          Length = 238

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GHMNMA 7
           GHMN+A
Sbjct: 136 GHMNIA 141



>XP_005276776
          Length = 991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GHMNMA 7
           GH+NMA
Sbjct: 270 GHINMA 275



>XP_005262564
          Length = 991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GHMNMA 7
           GH+NMA
Sbjct: 270 GHINMA 275



>XP_006718768
          Length = 483

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HMNMAFS 9
           H+N+AFS
Sbjct: 432 HINVAFS 438



>XP_006718767
          Length = 616

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HMNMAFS 9
           H+N+AFS
Sbjct: 565 HINVAFS 571



>XP_006718766
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HMNMAFS 9
           H+N+AFS
Sbjct: 612 HINVAFS 618



>XP_005262842
          Length = 782

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GGHMNMAFS 9
           GGH + A+S
Sbjct: 324 GGHYSSAYS 332



>XP_006710324
          Length = 943

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GHMNMA 7
           GH+NMA
Sbjct: 222 GHINMA 227



>NP_942581
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   HMNMAFS 9
           HM +AFS
Sbjct: 642 HMKLAFS 648



>NP_940967
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   HMNMAFS 9
           H+N+AF+
Sbjct: 209 HLNLAFN 215



>NP_060200
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   HMNMAFS 9
           HM +AFS
Sbjct: 642 HMKLAFS 648



>XP_006713275
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   HMNMAFS 9
           HM +AFS
Sbjct: 642 HMKLAFS 648



>XP_005265305
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   HMNMAFS 9
           HM +AFS
Sbjct: 642 HMKLAFS 648



>NP_001191006
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GHMNMAFS 9
           G+M MAFS
Sbjct: 958 GNMIMAFS 965



>NP_004779
          Length = 1073

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GHMNMAFS 9
           G+M MAFS
Sbjct: 965 GNMIMAFS 972



>NP_056140
          Length = 2266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    GHMNMAFS 9
            GH N+A++
Sbjct: 2154 GHANIAYT 2161



>NP_940852
          Length = 268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2  GHMNMAFS 9
          GH+ M F+
Sbjct: 59 GHLQMGFA 66



>XP_005262137
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2  GHMNMAFS 9
          GH+ M F+
Sbjct: 59 GHLQMGFA 66



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.132    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 568,177
Number of extensions: 892
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)