Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_056013                                                              18    1135
NP_001274365                                                           18    1135
XP_005271814                                                           18    1135
XP_005271813                                                           18    1135
XP_006715738                                                           18    1135
XP_005271812                                                           18    1135
XP_005271810                                                           18    1135
XP_005271809                                                           18    1135
XP_005271808                                                           18    1135
XP_005271807                                                           18    1135
NP_005742                                                              18    1935
NP_671714                                                              18    1935
XP_006715890                                                           18    1935
XP_005250160                                                           18    1935
XP_005250159                                                           18    1935
NP_004590                                                              16    5631
XP_006724373                                                           16    5631
NP_689991                                                              15    9605
NP_001073932                                                           15    9605
NP_001368                                                              15    9605
NP_776161                                                              15    9605
NP_001127812                                                           15    9605
NP_115678                                                              15    9605
NP_071423                                                              15    9605
NP_001273359                                                           15    9605
NP_620147                                                              15    9605
XP_006718966                                                           15    9605
XP_006718329                                                           15    9605
XP_006718328                                                           15    9605
XP_006715855                                                           15    9605
XP_006714730                                                           15    9605
XP_005272100                                                           15    9605
XP_005262954                                                           15    9605
XP_005262953                                                           15    9605
XP_005262950                                                           15    9605
>NP_056013
          Length = 1261

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GERSVLLAA 9
            GERS LLAA
Sbjct: 1148 GERSALLAA 1156
>NP_001274365
          Length = 1271

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GERSVLLAA 9
            GERS LLAA
Sbjct: 1205 GERSALLAA 1213
>XP_005271814
          Length = 1047

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GERSVLLAA 9
           GERS LLAA
Sbjct: 934 GERSALLAA 942
>XP_005271813
          Length = 1214

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GERSVLLAA 9
            GERS LLAA
Sbjct: 1148 GERSALLAA 1156
>XP_006715738
          Length = 1229

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GERSVLLAA 9
            GERS LLAA
Sbjct: 1163 GERSALLAA 1171
>XP_005271812
          Length = 1245

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GERSVLLAA 9
            GERS LLAA
Sbjct: 1220 GERSALLAA 1228
>XP_005271810
          Length = 1276

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GERSVLLAA 9
            GERS LLAA
Sbjct: 1163 GERSALLAA 1171
>XP_005271809
          Length = 1286

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GERSVLLAA 9
            GERS LLAA
Sbjct: 1220 GERSALLAA 1228
>XP_005271808
          Length = 1318

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GERSVLLAA 9
            GERS LLAA
Sbjct: 1205 GERSALLAA 1213
>XP_005271807
          Length = 1333

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GERSVLLAA 9
            GERS LLAA
Sbjct: 1220 GERSALLAA 1228
>NP_005742
          Length = 3907

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ERSVLLAA 9
            ERSVLLAA
Sbjct: 3037 ERSVLLAA 3044
>NP_671714
          Length = 3899

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ERSVLLAA 9
            ERSVLLAA
Sbjct: 3029 ERSVLLAA 3036
>XP_006715890
          Length = 3860

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ERSVLLAA 9
            ERSVLLAA
Sbjct: 2990 ERSVLLAA 2997
>XP_005250160
          Length = 3939

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ERSVLLAA 9
            ERSVLLAA
Sbjct: 3069 ERSVLLAA 3076
>XP_005250159
          Length = 3952

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ERSVLLAA 9
            ERSVLLAA
Sbjct: 3082 ERSVLLAA 3089
>NP_004590
          Length = 1141

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GERSVLLA 8
            G+R+VLLA
Sbjct: 1100 GQRAVLLA 1107
>XP_006724373
          Length = 1111

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GERSVLLA 8
            G+R+VLLA
Sbjct: 1070 GQRAVLLA 1077
>NP_689991
          Length = 518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GERSVLL 7
           GER++LL
Sbjct: 335 GERAILL 341
>NP_001073932
          Length = 4314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GERSVLL 7
            GE+SVLL
Sbjct: 2919 GEQSVLL 2925
>NP_001368
          Length = 4307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GERSVLL 7
            GE+SVLL
Sbjct: 2919 GEQSVLL 2925
>NP_776161
          Length = 990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GERSVLLAA 9
           GER  LLA+
Sbjct: 229 GERRALLAS 237
>NP_001127812
          Length = 122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GERSVLLA 8
          G R+VLLA
Sbjct: 91 GNRAVLLA 98
>NP_115678
          Length = 122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GERSVLLA 8
          G R+VLLA
Sbjct: 91 GNRAVLLA 98
>NP_071423
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GERSVL 6
           GERSVL
Sbjct: 608 GERSVL 613
>NP_001273359
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GERSVLLAA 9
           GE S+ LAA
Sbjct: 521 GEESISLAA 529
>NP_620147
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GERSVLLAA 9
           GE S+ LAA
Sbjct: 581 GEESISLAA 589
>XP_006718966
          Length = 4300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    GERSVLL 7
            GE+SVLL
Sbjct: 2912 GEQSVLL 2918
>XP_006718329
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GERSVL 6
           GERSVL
Sbjct: 390 GERSVL 395
>XP_006718328
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GERSVL 6
           GERSVL
Sbjct: 390 GERSVL 395
>XP_006715855
          Length = 122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GERSVLLA 8
          G R+VLLA
Sbjct: 91 GNRAVLLA 98
>XP_006714730
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GERSVL 6
           GERSVL
Sbjct: 235 GERSVL 240
>XP_005272100
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GERSVL 6
           GERSVL
Sbjct: 580 GERSVL 585
>XP_005262954
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GERSVLL 7
           GER++LL
Sbjct: 268 GERAILL 274
>XP_005262953
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GERSVLL 7
           GER++LL
Sbjct: 286 GERAILL 292
>XP_005262950
          Length = 518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GERSVLL 7
           GER++LL
Sbjct: 335 GERAILL 341
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.130    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 381,101
Number of extensions: 667
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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