Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              20     509
NP_009297                                                              20     509
NP_065849                                                              18    1935
NP_036286                                                              16    5631
NP_001158095                                                           16    5631
NP_036331                                                              16    5631
NP_001257569                                                           16    5631
NP_612207                                                              16    5631
NP_001257615                                                           16    5631
NP_001257570                                                           16    5631
NP_001257568                                                           16    5631
NP_036387                                                              16    5631
NP_001273331                                                           16    7355
NP_000237                                                              16    7355
NP_060527                                                              16    7355
NP_877499                                                              16    7355
NP_060099                                                              16    7355
NP_001034637                                                           16    7355
XP_006720946                                                           16    7355
XP_005255380                                                           16    7355
XP_006720945                                                           16    7355
XP_006720944                                                           16    7355
XP_005255378                                                           16    7355
XP_005255377                                                           16    7355
XP_006720943                                                           16    7355
XP_005270636                                                           16    7355
XP_005270635                                                           16    7355
NP_001138764                                                           15    9605
NP_015561                                                              15    9605
NP_000274                                                              15    9605
NP_001138763                                                           15    9605
NP_001364                                                              15    9605
NP_015559                                                              15    9605
NP_003557                                                              15    9605
XP_006719694                                                           15    9605
XP_005272337                                                           15    9605
XP_005272336                                                           15    9605
XP_006711798                                                           15    9605
NP_057301                                                              15    9605
>NP_005148
          Length = 1130

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   EAADEVFK 9
           EAADEVFK
Sbjct: 792 EAADEVFK 799
>NP_009297
          Length = 1149

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   EAADEVFK 9
           EAADEVFK
Sbjct: 811 EAADEVFK 818
>NP_065849
          Length = 1018

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   GEAADEVF 8
           G AADEVF
Sbjct: 114 GPAADEVF 121
>NP_036286
          Length = 859

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GEAADEVFK 9
           GE  D +FK
Sbjct: 121 GEGKDRIFK 129
>NP_001158095
          Length = 825

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GEAADEVFK 9
           GE  D +FK
Sbjct: 121 GEGKDRIFK 129
>NP_036331
          Length = 857

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GEAADEVFK 9
           GE  D +FK
Sbjct: 119 GEGKDRIFK 127
>NP_001257569
          Length = 188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EAADEVFK 9
          EAADE+ K
Sbjct: 41 EAADEICK 48
>NP_612207
          Length = 188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EAADEVFK 9
          EAADE+ K
Sbjct: 41 EAADEICK 48
>NP_001257615
          Length = 188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EAADEVFK 9
          EAADE+ K
Sbjct: 41 EAADEICK 48
>NP_001257570
          Length = 146

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EAADEVFK 9
          EAADE+ K
Sbjct: 41 EAADEICK 48
>NP_001257568
          Length = 188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EAADEVFK 9
          EAADE+ K
Sbjct: 41 EAADEICK 48
>NP_036387
          Length = 950

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GEAADEVFK 9
           GE  D +FK
Sbjct: 397 GEVEDSIFK 405
>NP_001273331
          Length = 1131

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AADEVF 8
           AADEVF
Sbjct: 480 AADEVF 485
>NP_000237
          Length = 1130

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AADEVF 8
           AADEVF
Sbjct: 479 AADEVF 484
>NP_060527
          Length = 289

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  EAADEVFK 9
          +AAD+ FK
Sbjct: 28 DAADDAFK 35
>NP_877499
          Length = 730

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  EAADEVFK 9
          +AAD+ FK
Sbjct: 28 DAADDAFK 35
>NP_060099
          Length = 861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GEAADEVFK 9
           GE  D+ FK
Sbjct: 111 GEGKDQTFK 119
>NP_001034637
          Length = 583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GEAADEVF 8
           GE+ D+VF
Sbjct: 543 GESTDKVF 550
>XP_006720946
          Length = 1081

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AADEVF 8
           AADEVF
Sbjct: 430 AADEVF 435
>XP_005255380
          Length = 1082

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AADEVF 8
           AADEVF
Sbjct: 431 AADEVF 436
>XP_006720945
          Length = 1096

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AADEVF 8
           AADEVF
Sbjct: 445 AADEVF 450
>XP_006720944
          Length = 1097

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AADEVF 8
           AADEVF
Sbjct: 446 AADEVF 451
>XP_005255378
          Length = 1145

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AADEVF 8
           AADEVF
Sbjct: 494 AADEVF 499
>XP_005255377
          Length = 1146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AADEVF 8
           AADEVF
Sbjct: 495 AADEVF 500
>XP_006720943
          Length = 1208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AADEVF 8
           AADEVF
Sbjct: 557 AADEVF 562
>XP_005270636
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  GEAADEVFK 9
          GE  D+ FK
Sbjct: 44 GEGKDQTFK 52
>XP_005270635
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  GEAADEVFK 9
          GE  D+ FK
Sbjct: 44 GEGKDQTFK 52
>NP_001138764
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  AADEVFK 9
          +ADE+FK
Sbjct: 82 SADEMFK 88
>NP_015561
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   EAADEV 7
           EAADEV
Sbjct: 222 EAADEV 227
>NP_000274
          Length = 854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AADEVFK 9
           +ADE+FK
Sbjct: 361 SADEMFK 367
>NP_001138763
          Length = 853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AADEVFK 9
           +ADE+FK
Sbjct: 361 SADEMFK 367
>NP_001364
          Length = 4515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    GEAADEVFK 9
            GE  +E+F+
Sbjct: 3960 GEVTEEIFE 3968
>NP_015559
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   EAADEV 7
           EAADEV
Sbjct: 154 EAADEV 159
>NP_003557
          Length = 1411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  AADEVFK 9
          +ADE+FK
Sbjct: 52 SADELFK 58
>XP_006719694
          Length = 1465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AADEVFK 9
           +ADE+FK
Sbjct: 106 SADELFK 112
>XP_005272337
          Length = 589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  AADEVFK 9
          +ADE+FK
Sbjct: 82 SADEMFK 88
>XP_005272336
          Length = 589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  AADEVFK 9
          +ADE+FK
Sbjct: 82 SADEMFK 88
>XP_006711798
          Length = 4586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    GEAADEVFK 9
            GE  +E+F+
Sbjct: 4022 GEVTEEIFE 4030
>NP_057301
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GEAADEVFK 9
           GEAA+E  K
Sbjct: 216 GEAAEEAKK 224
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.130    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 478,432
Number of extensions: 921
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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