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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              20     390
NP_055612                                                              19     869
NP_001157785                                                           19     869
NP_004637                                                              17    2528
XP_005259018                                                           17    2528
NP_001073289                                                           16    5631
NP_001120934                                                           16    5631
NP_004886                                                              16    5631
NP_001230062                                                           16    5631
NP_079291                                                              16    5631
NP_001265524                                                           16    5631
NP_877577                                                              16    5631
NP_005907                                                              16    5631
XP_006717810                                                           16    5631
XP_006717809                                                           16    5631
XP_005269730                                                           16    5631
XP_005269729                                                           16    5631
XP_005250405                                                           16    5631
XP_005273095                                                           16    5631
XP_005273094                                                           16    5631
XP_006711796                                                           16    5631
XP_005273093                                                           16    5631
NP_291025                                                              16    7355
NP_878907                                                              16    7355
NP_443202                                                              15    9605
NP_001184256                                                           15    9605
NP_004727                                                              15    9605
NP_001129141                                                           15    9605
XP_005260715                                                           15    9605
XP_005260713                                                           15    9605
XP_005260714                                                           15    9605

>NP_443179
          Length = 2170

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GDS VANKQ
Sbjct: 286 GDSAVANKQ 294



>NP_055612
          Length = 697

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GDSGV N+Q
Sbjct: 202 GDSGVLNEQ 210



>NP_001157785
          Length = 333

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GDSGV N+Q
Sbjct: 202 GDSGVLNEQ 210



>NP_004637
          Length = 1241

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GDSGVANK 8
            GDSG+A+K
Sbjct: 1020 GDSGLADK 1027



>XP_005259018
          Length = 1128

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GDSGVANK 8
           GDSG+A+K
Sbjct: 907 GDSGLADK 914



>NP_001073289
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GDSGVA 6
           GDSGVA
Sbjct: 868 GDSGVA 873



>NP_001120934
          Length = 979

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GDSGVA 6
           GDSGVA
Sbjct: 811 GDSGVA 816



>NP_004886
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GDSGVA 6
           GDSGVA
Sbjct: 868 GDSGVA 873



>NP_001230062
          Length = 1034

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GDSGVA 6
           GDSGVA
Sbjct: 866 GDSGVA 871



>NP_079291
          Length = 675

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GDSGVANKQ 9
          GD GV+N++
Sbjct: 90 GDPGVSNQK 98



>NP_001265524
          Length = 543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GDSGVANKQ 9
           GD GVA  Q
Sbjct: 205 GDPGVAKSQ 213



>NP_877577
          Length = 543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GDSGVANKQ 9
           GD GVA  Q
Sbjct: 205 GDPGVAKSQ 213



>NP_005907
          Length = 719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GDSGVANKQ 9
           GD GVA  Q
Sbjct: 381 GDPGVAKSQ 389



>XP_006717810
          Length = 691

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GD GV+N++
Sbjct: 106 GDPGVSNQK 114



>XP_006717809
          Length = 691

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GD GV+N++
Sbjct: 106 GDPGVSNQK 114



>XP_005269730
          Length = 691

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GD GV+N++
Sbjct: 106 GDPGVSNQK 114



>XP_005269729
          Length = 692

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GD GV+N++
Sbjct: 107 GDPGVSNQK 115



>XP_005250405
          Length = 612

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GDSGVANKQ 9
           GD GVA  Q
Sbjct: 274 GDPGVAKSQ 282



>XP_005273095
          Length = 979

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GDSGVA 6
           GDSGVA
Sbjct: 811 GDSGVA 816



>XP_005273094
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GDSGVA 6
           GDSGVA
Sbjct: 868 GDSGVA 873



>XP_006711796
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GDSGVA 6
           GDSGVA
Sbjct: 868 GDSGVA 873



>XP_005273093
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GDSGVA 6
           GDSGVA
Sbjct: 868 GDSGVA 873



>NP_291025
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SGVANKQ 9
           SGV NKQ
Sbjct: 712 SGVENKQ 718



>NP_878907
          Length = 544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   GDSGVANK 8
           GD  VANK
Sbjct: 518 GDKWVANK 525



>NP_443202
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GD GVA+++
Sbjct: 94  GDPGVAHQK 102



>NP_001184256
          Length = 685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GD GVA+++
Sbjct: 94  GDPGVAHQK 102



>NP_004727
          Length = 696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   DSGVANKQ 9
           D GV N+Q
Sbjct: 649 DDGVRNRQ 656



>NP_001129141
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   DSGVANKQ 9
           D GV N+Q
Sbjct: 584 DDGVRNRQ 591



>XP_005260715
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GD GVA+++
Sbjct: 94  GDPGVAHQK 102



>XP_005260713
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GDSGVANKQ 9
          GD GVA+++
Sbjct: 8  GDPGVAHQK 16



>XP_005260714
          Length = 664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GDSGVANKQ 9
           GD GVA+++
Sbjct: 94  GDPGVAHQK 102



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.121    0.321 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 537,009
Number of extensions: 740
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)