Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     869
NP_000242                                                              19     869
XP_005264389                                                           19     869
NP_001020774                                                           17    4312
NP_000440                                                              17    4312
XP_005245463                                                           17    4312
XP_005245462                                                           17    4312
NP_006436                                                              16    5631
NP_001361                                                              16    5631
XP_006712019                                                           16    5631
NP_065928                                                              16    7355
XP_005256527                                                           16    7355
NP_005888                                                              15    9605
NP_005959                                                              15    9605
NP_001093148                                                           15    9605
NP_563615                                                              15    9605
NP_945314                                                              15    9605
NP_112480                                                              15    9605
NP_001138821                                                           15    9605
XP_005272540                                                           15    9605
XP_005272539                                                           15    9605
XP_005272538                                                           15    9605
XP_005272537                                                           15    9605
XP_005272536                                                           15    9605
XP_005272535                                                           15    9605
XP_006710686                                                           15    9605
XP_005270897                                                           15    9605
XP_005270896                                                           15    9605
XP_006710685                                                           15    9605
NP_065954                                                              15    9605

>NP_001245210
          Length = 868

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GANNLQSVV 9
          GA NLQSVV
Sbjct: 5  GAKNLQSVV 13



>NP_000242
          Length = 934

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GANNLQSVV 9
          GA NLQSVV
Sbjct: 71 GAKNLQSVV 79



>XP_005264389
          Length = 921

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GANNLQSVV 9
          GA NLQSVV
Sbjct: 71 GAKNLQSVV 79



>NP_001020774
          Length = 616

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNLQ
Sbjct: 316 GANNLQ 321



>NP_000440
          Length = 616

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNLQ
Sbjct: 316 GANNLQ 321



>XP_005245463
          Length = 616

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNLQ
Sbjct: 316 GANNLQ 321



>XP_005245462
          Length = 616

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNLQ
Sbjct: 316 GANNLQ 321



>NP_006436
          Length = 2335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   GANNLQSV 8
           G NNLQ V
Sbjct: 958 GINNLQDV 965



>NP_001361
          Length = 4158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   NNLQSVV 9
           NNLQSV+
Sbjct: 846 NNLQSVL 852



>XP_006712019
          Length = 4158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   NNLQSVV 9
           NNLQSV+
Sbjct: 846 NNLQSVL 852



>NP_065928
          Length = 4427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    ANNLQSVV 9
            +NN+QS++
Sbjct: 2451 SNNVQSII 2458



>XP_005256527
          Length = 4427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    ANNLQSVV 9
            +NN+QS++
Sbjct: 2451 SNNVQSII 2458



>NP_005888
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   GANNLQSVV 9
           G NN+Q ++
Sbjct: 103 GVNNVQELI 111



>NP_005959
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNL+
Sbjct: 559 GANNLE 564



>NP_001093148
          Length = 371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   GANNLQSV 8
           G N LQS+
Sbjct: 151 GGNQLQSI 158



>NP_563615
          Length = 775

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ANNLQSVV 9
           ANN+ SVV
Sbjct: 270 ANNVTSVV 277



>NP_945314
          Length = 1386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    ANNLQSV 8
            A+NLQSV
Sbjct: 1014 AHNLQSV 1020



>NP_112480
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNL+
Sbjct: 520 GANNLE 525



>NP_001138821
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   GANNLQSVV 9
           G NN+Q ++
Sbjct: 103 GVNNVQELI 111



>XP_005272540
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNL+
Sbjct: 400 GANNLE 405



>XP_005272539
          Length = 595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNL+
Sbjct: 424 GANNLE 429



>XP_005272538
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNL+
Sbjct: 439 GANNLE 444



>XP_005272537
          Length = 676

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNL+
Sbjct: 505 GANNLE 510



>XP_005272536
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNL+
Sbjct: 541 GANNLE 546



>XP_005272535
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GANNLQ 6
           GANNL+
Sbjct: 544 GANNLE 549



>XP_006710686
          Length = 440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   GANNLQSVV 9
           G NN+Q ++
Sbjct: 103 GVNNVQELI 111



>XP_005270897
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   GANNLQSVV 9
           G NN+Q ++
Sbjct: 103 GVNNVQELI 111



>XP_005270896
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   GANNLQSVV 9
           G NN+Q ++
Sbjct: 103 GVNNVQELI 111



>XP_006710685
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   GANNLQSVV 9
           G NN+Q ++
Sbjct: 103 GVNNVQELI 111



>NP_065954
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ANNLQSVV 9
          +NN++SVV
Sbjct: 54 SNNIRSVV 61



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.121    0.304 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 398,251
Number of extensions: 475
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)