Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_835454                                                              18    1935
NP_037498                                                              17    4312
NP_001010876                                                           16    5631
NP_001230688                                                           16    5631
NP_004196                                                              16    5631
NP_741994                                                              16    5631
XP_005271779                                                           16    5631
XP_005271778                                                           16    5631
NP_775754                                                              16    7355
NP_001077086                                                           16    7355
NP_115971                                                              16    7355
NP_055596                                                              16    7355
NP_004496                                                              16    7355
XP_005256902                                                           16    7355
XP_006716556                                                           16    7355
XP_006716555                                                           16    7355
XP_006716554                                                           16    7355
NP_061111                                                              15    9605
NP_055245                                                              15    9605
NP_061830                                                              15    9605
NP_001230723                                                           15    9605
NP_002074                                                              15    9605
NP_001230724                                                           15    9605
XP_005267597                                                           15    9605
>NP_835454
          Length = 562

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FYRLYAVS 8
           FYR++AVS
Sbjct: 546 FYRMHAVS 553
>NP_037498
          Length = 822

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FYRLYA 6
           FYR+YA
Sbjct: 332 FYRIYA 337
>NP_001010876
          Length = 128

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FYRLYAVSL 9
          FY +Y VSL
Sbjct: 24 FYTVYYVSL 32
>NP_001230688
          Length = 362

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   YRLYAVS 8
           Y LYAVS
Sbjct: 298 YNLYAVS 304
>NP_004196
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   YRLYAVS 8
           Y LYAVS
Sbjct: 541 YNLYAVS 547
>NP_741994
          Length = 396

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   YRLYAVS 8
           Y LYAVS
Sbjct: 332 YNLYAVS 338
>XP_005271779
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   YRLYAVS 8
           Y LYAVS
Sbjct: 541 YNLYAVS 547
>XP_005271778
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   YRLYAVS 8
           Y LYAVS
Sbjct: 541 YNLYAVS 547
>NP_775754
          Length = 531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  YRLYAVS 8
          +R+YAVS
Sbjct: 19 FRIYAVS 25
>NP_001077086
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FYRLYAVS 8
            FYR+ AVS
Sbjct: 1579 FYRVKAVS 1586
>NP_115971
          Length = 1604

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    YRLYAVS 8
            Y LYA+S
Sbjct: 1510 YNLYAIS 1516
>NP_055596
          Length = 1594

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FYRLYAVS 8
            FYR+ AVS
Sbjct: 1582 FYRVKAVS 1589
>NP_004496
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    YRLYAVS 8
            Y LYA+S
Sbjct: 1312 YNLYAIS 1318
>XP_005256902
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    YRLYAVS 8
            Y LYA+S
Sbjct: 1312 YNLYAIS 1318
>XP_006716556
          Length = 1048

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FYRLYAVS 8
            FYR+ AVS
Sbjct: 1036 FYRVKAVS 1043
>XP_006716555
          Length = 1539

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FYRLYAVS 8
            FYR+ AVS
Sbjct: 1527 FYRVKAVS 1534
>XP_006716554
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FYRLYAVS 8
            FYR+ AVS
Sbjct: 1579 FYRVKAVS 1586
>NP_061111
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FYRLYAVSL 9
           FYR +AV +
Sbjct: 266 FYRKFAVPM 274
>NP_055245
          Length = 219

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FYRLYAVS 8
           FY LY++S
Sbjct: 156 FYGLYSMS 163
>NP_061830
          Length = 701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FYRLYAVSL 9
           F R+Y VSL
Sbjct: 270 FPRVYGVSL 278
>NP_001230723
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FYRLYAVSL 9
           FYR +AV +
Sbjct: 266 FYRKFAVPM 274
>NP_002074
          Length = 190

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FYRLYAVSL 9
          FY L A+SL
Sbjct: 8  FYDLSAISL 16
>NP_001230724
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FYRLYAVSL 9
           FYR +AV +
Sbjct: 266 FYRKFAVPM 274
>XP_005267597
          Length = 219

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FYRLYAVS 8
           FY LY++S
Sbjct: 156 FYGLYSMS 163
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.153    0.454 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 414,362
Number of extensions: 492
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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