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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              19     869
NP_705838                                                              16    5631
XP_005267426                                                           16    5631
NP_689660                                                              16    5631
XP_005269713                                                           16    7355
NP_058633                                                              15    9605
XP_005274609                                                           15    9605

>NP_004110
          Length = 993

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FVSSVARKD 9
           FVSSVAR D
Sbjct: 316 FVSSVARND 324



>NP_705838
          Length = 4069

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FVSSVARKD 9
            F+S V+R+D
Sbjct: 2280 FISEVSRED 2288



>XP_005267426
          Length = 466

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FVSSVARKD 9
          FV+++ RKD
Sbjct: 62 FVTNIKRKD 70



>NP_689660
          Length = 719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FVSSVARKD 9
          FV+++ RKD
Sbjct: 62 FVTNIKRKD 70



>XP_005269713
          Length = 1646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    FVSSVARKD 9
            F+S +A+K+
Sbjct: 1161 FISEIAKKE 1169



>NP_058633
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  FVSSVARKD 9
          FVSS AR++
Sbjct: 16 FVSSRARRE 24



>XP_005274609
          Length = 1468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  FVSSVARKD 9
          FVSS AR++
Sbjct: 22 FVSSRARRE 30



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.127    0.318 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 316,080
Number of extensions: 498
Number of successful extensions: 7
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)