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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_149037                                                              18    1135
NP_001171700                                                           18    1135
NP_005742                                                              18    1482
NP_671714                                                              18    1482
XP_006715890                                                           18    1482
XP_005250160                                                           18    1482
XP_005250159                                                           18    1482
NP_874364                                                              16    7355
NP_055877                                                              15    9605
NP_001275656                                                           15    9605
NP_001275657                                                           15    9605
NP_065761                                                              15    9605
NP_001034864                                                           15    9605
XP_006717794                                                           15    9605
XP_005269715                                                           15    9605
XP_005245975                                                           15    9605

>NP_149037
          Length = 301

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FVSQIQLEA 9
           FVSQIQ+E+
Sbjct: 238 FVSQIQIES 246



>NP_001171700
          Length = 301

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FVSQIQLEA 9
           FVSQIQ+E+
Sbjct: 238 FVSQIQIES 246



>NP_005742
          Length = 3907

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    VSQIQLEA 9
            VSQIQLEA
Sbjct: 2556 VSQIQLEA 2563



>NP_671714
          Length = 3899

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    VSQIQLEA 9
            VSQIQLEA
Sbjct: 2548 VSQIQLEA 2555



>XP_006715890
          Length = 3860

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    VSQIQLEA 9
            VSQIQLEA
Sbjct: 2509 VSQIQLEA 2516



>XP_005250160
          Length = 3939

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    VSQIQLEA 9
            VSQIQLEA
Sbjct: 2588 VSQIQLEA 2595



>XP_005250159
          Length = 3952

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    VSQIQLEA 9
            VSQIQLEA
Sbjct: 2601 VSQIQLEA 2608



>NP_874364
          Length = 214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FVSQIQLEA 9
           F+S ++LEA
Sbjct: 193 FLSSVELEA 201



>NP_055877
          Length = 1664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FVSQIQLEA 9
            F+S+I +EA
Sbjct: 1161 FISEIGIEA 1169



>NP_001275656
          Length = 1400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FVSQIQLEA 9
            F+S+I +EA
Sbjct: 1161 FISEIGIEA 1169



>NP_001275657
          Length = 1542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FVSQIQLEA 9
            F+S+I +EA
Sbjct: 1041 FISEIGIEA 1049



>NP_065761
          Length = 583

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSQIQLEA 9
           V Q+QLEA
Sbjct: 413 VRQLQLEA 420



>NP_001034864
          Length = 1661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    FVSQIQL 7
            FVS+IQL
Sbjct: 1354 FVSKIQL 1360



>XP_006717794
          Length = 1194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FVSQIQLEA 9
            F+S+I +EA
Sbjct: 1161 FISEIGIEA 1169



>XP_005269715
          Length = 1230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FVSQIQLEA 9
            F+S+I +EA
Sbjct: 1161 FISEIGIEA 1169



>XP_005245975
          Length = 1661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    FVSQIQL 7
            FVS+IQL
Sbjct: 1354 FVSKIQL 1360



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.133    0.317 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 330,794
Number of extensions: 422
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)