Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_060791                                                              17    2528
NP_619732                                                              17    4312
NP_443193                                                              17    4312
NP_001180405                                                           17    4312
XP_006715059                                                           17    4312
NP_004451                                                              16    5631
NP_115785                                                              16    7355
NP_000129                                                              16    7355
NP_055383                                                              16    7355
NP_004685                                                              15    9605
NP_001167637                                                           15    9605
NP_699196                                                              15    9605
NP_055401                                                              15    9605
XP_006722232                                                           15    9605
XP_005257846                                                           15    9605
XP_005248199                                                           15    9605
XP_005248198                                                           15    9605
XP_005248197                                                           15    9605
XP_005248196                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FTVLSNRIT 9
            FTVLS+RIT
Sbjct: 1478 FTVLSDRIT 1486
>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FTVLSNRIT 9
            FTVLS+RIT
Sbjct: 1273 FTVLSDRIT 1281
>NP_060791
          Length = 353

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FTVLSNRI 8
           FT+L NRI
Sbjct: 208 FTILDNRI 215
>NP_619732
          Length = 865

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   FTVLSNRIT 9
           FTV++N+I+
Sbjct: 213 FTVIANKIS 221
>NP_443193
          Length = 970

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   FTVLSNRIT 9
           FTV++N+I+
Sbjct: 318 FTVIANKIS 326
>NP_001180405
          Length = 970

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   FTVLSNRIT 9
           FTV++N+I+
Sbjct: 318 FTVIANKIS 326
>XP_006715059
          Length = 1042

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   FTVLSNRIT 9
           FTV++N+I+
Sbjct: 390 FTVIANKIS 398
>NP_004451
          Length = 760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FTVLSNR 7
          +T+LSNR
Sbjct: 87 YTILSNR 93
>NP_115785
          Length = 581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TVLSNRIT 9
           T+L+NR+T
Sbjct: 538 TLLANRLT 545
>NP_000129
          Length = 2871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FTVLSNRI 8
           FTV+SN++
Sbjct: 187 FTVISNQM 194
>NP_055383
          Length = 940

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FTVLSNRI 8
           FTV SNR+
Sbjct: 281 FTVNSNRL 288
>NP_004685
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TVLSNR 7
          TVLSNR
Sbjct: 82 TVLSNR 87
>NP_001167637
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TVLSNR 7
          TVLSNR
Sbjct: 82 TVLSNR 87
>NP_699196
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTVLSNRI 8
           FT+  NRI
Sbjct: 559 FTICGNRI 566
>NP_055401
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TVLSNR 7
           TVLSNR
Sbjct: 365 TVLSNR 370
>XP_006722232
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TVLSNR 7
          TVLSNR
Sbjct: 82 TVLSNR 87
>XP_005257846
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TVLSNR 7
          TVLSNR
Sbjct: 82 TVLSNR 87
>XP_005248199
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTVLSNRI 8
           FT+  NRI
Sbjct: 418 FTICGNRI 425
>XP_005248198
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTVLSNRI 8
           FT+  NRI
Sbjct: 418 FTICGNRI 425
>XP_005248197
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTVLSNRI 8
           FT+  NRI
Sbjct: 433 FTICGNRI 440
>XP_005248196
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTVLSNRI 8
           FT+  NRI
Sbjct: 433 FTICGNRI 440
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.137    0.360 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 306,951
Number of extensions: 322
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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