Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              21     175
XP_005262424                                                           21     175
NP_006802                                                              17    3301
NP_945179                                                              17    3301
NP_061146                                                              16    5631
NP_954872                                                              16    5631
NP_001080                                                              16    7355
NP_004657                                                              15    9605
NP_945349                                                              15    9605
NP_067585                                                              15    9605
NP_004318                                                              15    9605
NP_001164509                                                           15    9605
NP_058633                                                              15    9605
NP_004034                                                              15    9605
NP_001095937                                                           15    9605
NP_060417                                                              15    9605
NP_071337                                                              15    9605
XP_006724996                                                           15    9605
XP_006724562                                                           15    9605
XP_005274609                                                           15    9605
NP_001164510                                                           15    9605
NP_001127694                                                           15    9605
NP_150366                                                              15    9605
NP_631948                                                              15    9605
>NP_722576
          Length = 3080

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FTSAFYENT 9
           FTSAF+ENT
Sbjct: 682 FTSAFHENT 690
>XP_005262424
          Length = 2875

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FTSAFYENT 9
           FTSAF+ENT
Sbjct: 477 FTSAFHENT 485
>NP_006802
          Length = 473

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FTSAFY 6
           FTSAFY
Sbjct: 209 FTSAFY 214
>NP_945179
          Length = 473

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FTSAFY 6
           FTSAFY
Sbjct: 209 FTSAFY 214
>NP_061146
          Length = 852

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AFYENT 9
           AFY+NT
Sbjct: 810 AFYDNT 815
>NP_954872
          Length = 799

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AFYENT 9
           AFY+NT
Sbjct: 757 AFYDNT 762
>NP_001080
          Length = 1704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SAFYEN 8
            S+FYEN
Sbjct: 1262 SSFYEN 1267
>NP_004657
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  FTSAFYEN 8
          FT+A YE+
Sbjct: 25 FTAAVYEH 32
>NP_945349
          Length = 295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FTSAFY 6
           FTSAF+
Sbjct: 194 FTSAFF 199
>NP_067585
          Length = 1227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    FTSAFYEN 8
            FT  FY N
Sbjct: 1096 FTDEFYPN 1103
>NP_004318
          Length = 1271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    FTSAFYEN 8
            FT  FY N
Sbjct: 1140 FTDEFYPN 1147
>NP_001164509
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  AFYENT 9
          AFY NT
Sbjct: 82 AFYRNT 87
>NP_058633
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   AFYEN 8
           AFYEN
Sbjct: 795 AFYEN 799
>NP_004034
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  AFYENT 9
          AFY NT
Sbjct: 82 AFYRNT 87
>NP_001095937
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FTSAFY 6
           FTSAF+
Sbjct: 206 FTSAFF 211
>NP_060417
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  FTSAFYENT 9
          FTS   ENT
Sbjct: 68 FTSDLMENT 76
>NP_071337
          Length = 408

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   FTSAFY 6
           FT AFY
Sbjct: 97  FTGAFY 102
>XP_006724996
          Length = 181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1  FTSAFYEN 8
          FT  FY N
Sbjct: 26 FTDEFYPN 33
>XP_006724562
          Length = 1326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   AFYEN 8
           AFYEN
Sbjct: 659 AFYEN 663
>XP_005274609
          Length = 1468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   AFYEN 8
           AFYEN
Sbjct: 801 AFYEN 805
>NP_001164510
          Length = 298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  AFYENT 9
          AFY NT
Sbjct: 82 AFYRNT 87
>NP_001127694
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTSAFYEN 8
           +TS FY N
Sbjct: 384 YTSPFYGN 391
>NP_150366
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTSAFYEN 8
           +TS FY N
Sbjct: 384 YTSPFYGN 391
>NP_631948
          Length = 553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TSAFYE 7
           TSAFY+
Sbjct: 168 TSAFYD 173
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.125    0.361 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 484,909
Number of extensions: 830
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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