Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              20     390
NP_000072                                                              16    7355
NP_443179                                                              16    7355
NP_112477                                                              16    7355
NP_001243298                                                           16    7355
NP_071429                                                              16    7355
XP_005266703                                                           16    7355
XP_006711794                                                           16    7355
XP_005273086                                                           16    7355
XP_005273085                                                           16    7355
XP_005273084                                                           16    7355
NP_006632                                                              16    7355
NP_001135817                                                           15    9605
NP_001135815                                                           15    9605
NP_001135816                                                           15    9605
NP_004063                                                              15    9605
NP_001004705                                                           15    9605
NP_001004715                                                           15    9605
>NP_004110
          Length = 993

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FTKMFMLNI 9
           FTKMF LNI
Sbjct: 427 FTKMFTLNI 435
>NP_000072
          Length = 3801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    FTKMFM 6
            FTKMF+
Sbjct: 3253 FTKMFL 3258
>NP_443179
          Length = 2170

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FTKMFMLNI 9
           FT  F LNI
Sbjct: 890 FTSTFTLNI 898
>NP_112477
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TKMFMLNI 9
          T MF+LN+
Sbjct: 83 TDMFLLNL 90
>NP_001243298
          Length = 357

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TKMFMLNI 9
          T MF+LN+
Sbjct: 71 TDMFLLNL 78
>NP_071429
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TKMFMLNI 9
          T MF+LN+
Sbjct: 78 TNMFILNL 85
>XP_005266703
          Length = 1501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FTKMFMLNI 9
           FT  F LNI
Sbjct: 221 FTSTFTLNI 229
>XP_006711794
          Length = 3022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    FTKMFM 6
            FTKMF+
Sbjct: 2474 FTKMFL 2479
>XP_005273086
          Length = 3264

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    FTKMFM 6
            FTKMF+
Sbjct: 3253 FTKMFL 3258
>XP_005273085
          Length = 3755

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    FTKMFM 6
            FTKMF+
Sbjct: 3207 FTKMFL 3212
>XP_005273084
          Length = 3801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    FTKMFM 6
            FTKMF+
Sbjct: 3253 FTKMFL 3258
>NP_006632
          Length = 357

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TKMFMLNI 9
          T MF+LN+
Sbjct: 71 TDMFLLNL 78
>NP_001135817
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FTKMFMLNI 9
           FT +F+L I
Sbjct: 124 FTSVFLLTI 132
>NP_001135815
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FTKMFMLNI 9
           FT +F+L I
Sbjct: 124 FTSVFLLTI 132
>NP_001135816
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FTKMFMLNI 9
           FT +F+L I
Sbjct: 124 FTSVFLLTI 132
>NP_004063
          Length = 371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FTKMFMLNI 9
           FT +F+L I
Sbjct: 122 FTSVFLLTI 130
>NP_001004705
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FTKMFMLNI 9
           FT+MF+ ++
Sbjct: 98  FTQMFLFHL 106
>NP_001004715
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   FTKMFMLNI 9
           FT++F+L++
Sbjct: 129 FTQIFLLHL 137
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.354    0.152    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 297,468
Number of extensions: 442
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)

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