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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_037536                                                              18    1482
NP_005814                                                              18    1482
NP_001170826                                                           18    1482
XP_005255091                                                           18    1482
XP_006720900                                                           18    1482
XP_006720899                                                           18    1482
NP_001727                                                              15    9605
NP_766638                                                              15    9605
XP_005261809                                                           15    9605
XP_005259247                                                           15    9605

>NP_037536
          Length = 630

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TFMTLRTD 9
           TFM LRTD
Sbjct: 530 TFMKLRTD 537



>NP_005814
          Length = 622

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TFMTLRTD 9
           TFM LRTD
Sbjct: 522 TFMKLRTD 529



>NP_001170826
          Length = 622

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TFMTLRTD 9
           TFM LRTD
Sbjct: 522 TFMKLRTD 529



>XP_005255091
          Length = 621

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TFMTLRTD 9
           TFM LRTD
Sbjct: 521 TFMKLRTD 528



>XP_006720900
          Length = 622

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TFMTLRTD 9
           TFM LRTD
Sbjct: 522 TFMKLRTD 529



>XP_006720899
          Length = 622

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TFMTLRTD 9
           TFM LRTD
Sbjct: 522 TFMKLRTD 529



>NP_001727
          Length = 350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FTFMTLRT 8
           +TF+ LRT
Sbjct: 222 YTFILLRT 229



>NP_766638
          Length = 988

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TFMTLRTD 9
           TF+TL+ D
Sbjct: 522 TFVTLKCD 529



>XP_005261809
          Length = 1018

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TFMTLRTD 9
           TF+TL+ D
Sbjct: 552 TFVTLKCD 559



>XP_005259247
          Length = 231

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FTFMTLRT 8
           +TF+ LRT
Sbjct: 103 YTFILLRT 110



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.145    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 348,132
Number of extensions: 356
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)