Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_005884                                                              17    4312
NP_919289                                                              16    5631
XP_005251508                                                           16    5631
XP_005251507                                                           16    5631
NP_001025054                                                           16    7355
NP_115878                                                              16    7355
NP_005793                                                              15    9605
NP_001182551                                                           15    9605
NP_001006642                                                           15    9605
NP_001252543                                                           15    9605
NP_001006643                                                           15    9605
NP_443133                                                              15    9605
XP_005251746                                                           15    9605
XP_006717012                                                           15    9605
XP_006717011                                                           15    9605
XP_005251745                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TASDRQTK 9
           TASDRQTK
Sbjct: 920 TASDRQTK 927
>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TASDRQTK 9
           TASDRQTK
Sbjct: 920 TASDRQTK 927
>NP_005884
          Length = 588

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TASDRQTK 9
           TASD QTK
Sbjct: 535 TASDVQTK 542
>NP_919289
          Length = 1368

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SDRQTK 9
           SDRQTK
Sbjct: 464 SDRQTK 469
>XP_005251508
          Length = 1007

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SDRQTK 9
           SDRQTK
Sbjct: 103 SDRQTK 108
>XP_005251507
          Length = 1079

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SDRQTK 9
           SDRQTK
Sbjct: 175 SDRQTK 180
>NP_001025054
          Length = 1288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    TASDRQ 7
            TASDRQ
Sbjct: 1193 TASDRQ 1198
>NP_115878
          Length = 543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FTASDRQT 8
           FT ++RQT
Sbjct: 157 FTVTERQT 164
>NP_005793
          Length = 1045

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ASDRQTK 9
           +SDR+TK
Sbjct: 848 SSDRETK 854
>NP_001182551
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ASDRQTK 9
           +SDR+TK
Sbjct: 783 SSDRETK 789
>NP_001006642
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTASDRQT 8
           FT  +RQT
Sbjct: 210 FTVEERQT 217
>NP_001252543
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTASDRQT 8
           FT  +RQT
Sbjct: 208 FTVEERQT 215
>NP_001006643
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTASDRQT 8
           FT  +RQT
Sbjct: 196 FTVEERQT 203
>NP_443133
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTASDRQT 8
           FT  +RQT
Sbjct: 176 FTVEERQT 183
>XP_005251746
          Length = 366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  FTASDRQT 8
          FT  +RQT
Sbjct: 73 FTVEERQT 80
>XP_006717012
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  FTASDRQT 8
          FT  +RQT
Sbjct: 85 FTVEERQT 92
>XP_006717011
          Length = 481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTASDRQT 8
           FT  +RQT
Sbjct: 188 FTVEERQT 195
>XP_005251745
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FTASDRQT 8
           FT  +RQT
Sbjct: 222 FTVEERQT 229
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.115    0.299 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 392,165
Number of extensions: 369
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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