Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
XP_005267581                                                           17    4312
NP_001273331                                                           16    5631
NP_000237                                                              16    5631
NP_003254                                                              16    5631
NP_001159588                                                           16    5631
NP_003266                                                              16    5631
XP_006720946                                                           16    5631
XP_005255380                                                           16    5631
XP_006720945                                                           16    5631
XP_006720944                                                           16    5631
XP_005255378                                                           16    5631
XP_005255377                                                           16    5631
XP_006720943                                                           16    5631
XP_005262723                                                           16    5631
XP_005262722                                                           16    5631
XP_006714091                                                           16    5631
XP_005262720                                                           16    5631
XP_005262719                                                           16    5631
NP_056004                                                              16    7355
XP_006712033                                                           16    7355
XP_005264280                                                           16    7355
NP_008851                                                              15    9605
NP_001189364                                                           15    9605
NP_001159436                                                           15    9605
NP_001159435                                                           15    9605
NP_002438                                                              15    9605
NP_001231866                                                           15    9605
NP_005087                                                              15    9605
NP_036292                                                              15    9605
NP_001265645                                                           15    9605
NP_660152                                                              15    9605
NP_001164634                                                           15    9605
NP_001164633                                                           15    9605
NP_963893                                                              15    9605
XP_005262367                                                           15    9605
XP_006724778                                                           15    9605
XP_005262366                                                           15    9605
XP_005266987                                                           15    9605
XP_006715521                                                           15    9605
XP_005265227                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    FSVLGTRMT 9
            F VLGTRMT
Sbjct: 1863 FPVLGTRMT 1871
>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    FSVLGTRMT 9
            F VLGTRMT
Sbjct: 1658 FPVLGTRMT 1666
>XP_005267581
          Length = 509

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FSVLGTRM 8
          FS+LGT M
Sbjct: 7  FSLLGTEM 14
>NP_001273331
          Length = 1131

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 835 SFLGTRLT 842
>NP_000237
          Length = 1130

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 834 SFLGTRLT 841
>NP_003254
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FSVLGTRM 8
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>NP_001159588
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FSVLGTR 7
           FS++GTR
Sbjct: 229 FSIVGTR 235
>NP_003266
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FSVLGTR 7
           FS++GTR
Sbjct: 229 FSIVGTR 235
>XP_006720946
          Length = 1081

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 785 SFLGTRLT 792
>XP_005255380
          Length = 1082

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 786 SFLGTRLT 793
>XP_006720945
          Length = 1096

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 800 SFLGTRLT 807
>XP_006720944
          Length = 1097

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 801 SFLGTRLT 808
>XP_005255378
          Length = 1145

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 849 SFLGTRLT 856
>XP_005255377
          Length = 1146

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 850 SFLGTRLT 857
>XP_006720943
          Length = 1208

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 912 SFLGTRLT 919
>XP_005262723
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FSVLGTRM 8
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>XP_005262722
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FSVLGTRM 8
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>XP_006714091
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FSVLGTRM 8
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>XP_005262720
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FSVLGTRM 8
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>XP_005262719
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FSVLGTRM 8
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>NP_056004
          Length = 811

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FSVLGTRMT 9
           +SVLG R T
Sbjct: 302 YSVLGARET 310
>XP_006712033
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FSVLGTRMT 9
           +SVLG R T
Sbjct: 160 YSVLGARET 168
>XP_005264280
          Length = 815

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FSVLGTRMT 9
           +SVLG R T
Sbjct: 302 YSVLGARET 310
>NP_008851
          Length = 1998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FSVLGTRMT 9
           FS+ G R+T
Sbjct: 536 FSIEGNRLT 544
>NP_001189364
          Length = 2009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FSVLGTRMT 9
           FS+ G R+T
Sbjct: 536 FSIEGNRLT 544
>NP_001159436
          Length = 1981

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FSVLGTRMT 9
           FS+ G R+T
Sbjct: 536 FSIEGNRLT 544
>NP_001159435
          Length = 2009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FSVLGTRMT 9
           FS+ G R+T
Sbjct: 536 FSIEGNRLT 544
>NP_002438
          Length = 1400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 180 SPLGTRVT 187
>NP_001231866
          Length = 1351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 180 SPLGTRVT 187
>NP_005087
          Length = 1370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S +GT+MT
Sbjct: 303 SAVGTKMT 310
>NP_036292
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VLGTRM 8
           +LGTRM
Sbjct: 566 ILGTRM 571
>NP_001265645
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VLGTRM 8
           +LGTRM
Sbjct: 566 ILGTRM 571
>NP_660152
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FSVLGT 6
          FS+LGT
Sbjct: 47 FSILGT 52
>NP_001164634
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S +GT+MT
Sbjct: 303 SAVGTKMT 310
>NP_001164633
          Length = 1358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S +GT+MT
Sbjct: 303 SAVGTKMT 310
>NP_963893
          Length = 1370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S +GT+MT
Sbjct: 303 SAVGTKMT 310
>XP_005262367
          Length = 1358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S +GT+MT
Sbjct: 303 SAVGTKMT 310
>XP_006724778
          Length = 1370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S +GT+MT
Sbjct: 303 SAVGTKMT 310
>XP_005262366
          Length = 1370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S +GT+MT
Sbjct: 303 SAVGTKMT 310
>XP_005266987
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VLGTRM 8
           +LGTRM
Sbjct: 542 ILGTRM 547
>XP_006715521
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VLGTRM 8
           +LGTRM
Sbjct: 566 ILGTRM 571
>XP_005265227
          Length = 1401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SVLGTRMT 9
           S LGTR+T
Sbjct: 180 SPLGTRVT 187
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.137    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 396,962
Number of extensions: 535
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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