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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              18    1482
NP_001123292                                                           17    3301
XP_005258025                                                           17    3301
NP_036347                                                              17    4312
NP_037408                                                              16    7355
NP_001361                                                              16    7355
NP_001164224                                                           16    7355
NP_659454                                                              16    7355
XP_006712019                                                           16    7355
NP_057079                                                              16    7355
NP_008986                                                              15    9605
NP_113626                                                              15    9605
XP_006717660                                                           15    9605
XP_005272154                                                           15    9605
XP_005272153                                                           15    9605
XP_005272152                                                           15    9605

>NP_004251
          Length = 1208

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    FSSRAVAHI 9
            FSSRAVA I
Sbjct: 1155 FSSRAVARI 1163



>NP_001123292
          Length = 912

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FSSRAVAHI 9
           F+SRA+ H+
Sbjct: 894 FASRAIKHL 902



>XP_005258025
          Length = 912

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FSSRAVAHI 9
           F+SRA+ H+
Sbjct: 894 FASRAIKHL 902



>NP_036347
          Length = 916

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FSSRAVAH 8
           +SSR VAH
Sbjct: 397 YSSRQVAH 404



>NP_037408
          Length = 478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FSSRAVAHI 9
           F  RAV+H+
Sbjct: 116 FEPRAVSHL 124



>NP_001361
          Length = 4158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SSRAVAHI 9
           S RAV HI
Sbjct: 237 SQRAVTHI 244



>NP_001164224
          Length = 405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSRAVAH 8
           SSR VAH
Sbjct: 398 SSRCVAH 404



>NP_659454
          Length = 425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSRAVAH 8
           SSR VAH
Sbjct: 418 SSRCVAH 424



>XP_006712019
          Length = 4158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SSRAVAHI 9
           S RAV HI
Sbjct: 237 SQRAVTHI 244



>NP_057079
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  SSRAVAH 8
          SSRAV H
Sbjct: 19 SSRAVVH 25



>NP_008986
          Length = 1390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SRAVAHI 9
            SRAV HI
Sbjct: 1208 SRAVIHI 1214



>NP_113626
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SRAVAH 8
           SRA+AH
Sbjct: 444 SRAIAH 449



>XP_006717660
          Length = 1369

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SRAVAHI 9
            SRAV HI
Sbjct: 1187 SRAVIHI 1193



>XP_005272154
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SRAVAH 8
           SRA+AH
Sbjct: 426 SRAIAH 431



>XP_005272153
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SRAVAH 8
           SRA+AH
Sbjct: 426 SRAIAH 431



>XP_005272152
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SRAVAH 8
           SRA+AH
Sbjct: 444 SRAIAH 449



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.128    0.354 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 353,138
Number of extensions: 477
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)