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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001121619                                                           18    1135
NP_000258                                                              18    1135
NP_001035957                                                           18    1135
XP_006721991                                                           18    1135
XP_006721990                                                           18    1135
XP_006721989                                                           18    1135
XP_005258041                                                           18    1135
XP_006721988                                                           18    1135
XP_006721987                                                           18    1135
XP_006721986                                                           18    1135
XP_005258040                                                           18    1135
XP_006721985                                                           18    1135
NP_612376                                                              17    4312
XP_006725123                                                           17    4312
XP_006725122                                                           17    4312
XP_006716744                                                           17    4312
XP_005272410                                                           17    4312
XP_005272409                                                           17    4312
NP_002510                                                              16    5631
NP_998770                                                              16    7355
XP_006716616                                                           16    7355
NP_002011                                                              15    9605
NP_891555                                                              15    9605
NP_612456                                                              15    9605
NP_009084                                                              15    9605
NP_078947                                                              15    9605

>NP_001121619
          Length = 593

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>NP_000258
          Length = 2818

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>NP_001035957
          Length = 2839

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>XP_006721991
          Length = 1632

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>XP_006721990
          Length = 2823

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>XP_006721989
          Length = 2823

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>XP_005258041
          Length = 2836

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>XP_006721988
          Length = 2846

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>XP_006721987
          Length = 2849

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>XP_006721986
          Length = 2854

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 141 FSRISTRLQ 149



>XP_005258040
          Length = 2857

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>XP_006721985
          Length = 2867

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSRIST LQ
Sbjct: 154 FSRISTRLQ 162



>NP_612376
          Length = 671

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 302 FSRSSTLIQ 310




 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 498 FSRRSTLIQ 506



>XP_006725123
          Length = 671

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 302 FSRSSTLIQ 310




 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 498 FSRRSTLIQ 506



>XP_006725122
          Length = 671

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 302 FSRSSTLIQ 310




 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 498 FSRRSTLIQ 506



>XP_006716744
          Length = 671

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 302 FSRSSTLIQ 310




 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 498 FSRRSTLIQ 506



>XP_005272410
          Length = 671

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 302 FSRSSTLIQ 310




 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 498 FSRRSTLIQ 506



>XP_005272409
          Length = 671

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 302 FSRSSTLIQ 310




 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FSR ST++Q
Sbjct: 498 FSRRSTLIQ 506



>NP_002510
          Length = 1427

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FS+IST +Q
Sbjct: 416 FSQISTSIQ 424



>NP_998770
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SRISTMLQ 9
           SR+ST++Q
Sbjct: 462 SRLSTLIQ 469



>XP_006716616
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SRISTMLQ 9
           SR+ST++Q
Sbjct: 462 SRLSTLIQ 469



>NP_002011
          Length = 1298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    FSRISTM 7
            FS++STM
Sbjct: 1199 FSQVSTM 1205



>NP_891555
          Length = 1363

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    FSRISTM 7
            FS++STM
Sbjct: 1199 FSQVSTM 1205



>NP_612456
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           FS+ ST+LQ
Sbjct: 354 FSQRSTLLQ 362



>NP_009084
          Length = 1280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   FSRISTML 8
           FS++ST++
Sbjct: 322 FSKVSTLI 329



>NP_078947
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FSRISTMLQ 9
           F+R ST++Q
Sbjct: 466 FNRSSTLIQ 474



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.133    0.330 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 301,733
Number of extensions: 819
Number of successful extensions: 32
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)