Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           22     134
NP_001257437                                                           22     134
NP_006281                                                              22     134
XP_006715618                                                           22     134
XP_005267176                                                           22     134
NP_387450                                                              17    2528
NP_004352                                                              17    2528
NP_001004301                                                           17    4312
NP_055061                                                              17    4312
XP_006724446                                                           17    4312
XP_006723069                                                           17    4312
NP_001020402                                                           16    5631
NP_001095886                                                           16    5631
NP_002829                                                              16    5631
NP_001139693                                                           16    5631
XP_006719087                                                           16    5631
XP_006711535                                                           16    5631
NP_001189386                                                           16    7355
NP_001189385                                                           16    7355
NP_001026835                                                           16    7355
NP_061825                                                              15    9605
NP_001245283                                                           15    9605
NP_004238                                                              15    9605
NP_001245282                                                           15    9605
NP_001136077                                                           15    9605
NP_001099003                                                           15    9605
NP_443159                                                              15    9605
NP_079189                                                              15    9605
NP_001253964                                                           15    9605
XP_005257340                                                           15    9605
XP_006721919                                                           15    9605
XP_005257341                                                           15    9605
XP_005265867                                                           15    9605
XP_005265868                                                           15    9605
XP_005265869                                                           15    9605
XP_005265866                                                           15    9605
XP_005265865                                                           15    9605
XP_005265864                                                           15    9605
NP_001124336                                                           15    9605
>NP_001257436
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSETTTMKC 9
           FSETT MKC
Sbjct: 470 FSETTAMKC 478
>NP_001257437
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSETTTMKC 9
           FSETT MKC
Sbjct: 470 FSETTAMKC 478
>NP_006281
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSETTTMKC 9
           FSETT MKC
Sbjct: 470 FSETTAMKC 478
>XP_006715618
          Length = 577

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSETTTMKC 9
           FSETT MKC
Sbjct: 257 FSETTAMKC 265
>XP_005267176
          Length = 790

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FSETTTMKC 9
           FSETT MKC
Sbjct: 470 FSETTAMKC 478
>NP_387450
          Length = 785

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FSETTTMK 8
           FS+TTT+K
Sbjct: 358 FSDTTTVK 365
>NP_004352
          Length = 785

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FSETTTMK 8
           FS+TTT+K
Sbjct: 358 FSDTTTVK 365
>NP_001004301
          Length = 617

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FSETTTMKC 9
           FS+  T+KC
Sbjct: 392 FSQELTLKC 400
>NP_055061
          Length = 3014

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FSETTTMKC 9
            F+E TT+ C
Sbjct: 2040 FAEVTTLGC 2048
>XP_006724446
          Length = 3019

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FSETTTMKC 9
            F+E TT+ C
Sbjct: 2040 FAEVTTLGC 2048
>XP_006723069
          Length = 533

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FSETTTMKC 9
           FS+  T+KC
Sbjct: 392 FSQELTLKC 400
>NP_001020402
          Length = 579

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SETTTMKC 9
          S+TT +KC
Sbjct: 67 SQTTQVKC 74
>NP_001095886
          Length = 644

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FSETTTMKC 9
           F +TT+ KC
Sbjct: 606 FPDTTSPKC 614
>NP_002829
          Length = 1306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  FSETTT 6
          FSETTT
Sbjct: 69 FSETTT 74
>NP_001139693
          Length = 340

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FSETTTMKC 9
          +SE+T  KC
Sbjct: 59 YSESTGQKC 67
>XP_006719087
          Length = 295

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FSETTTMKC 9
          +SE+T  KC
Sbjct: 59 YSESTGQKC 67
>XP_006711535
          Length = 1258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  FSETTT 6
          FSETTT
Sbjct: 69 FSETTT 74
>NP_001189386
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FSETTTMKC 9
           FSE ++++C
Sbjct: 322 FSEKSSLRC 330
>NP_001189385
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FSETTTMKC 9
           FSE ++++C
Sbjct: 322 FSEKSSLRC 330
>NP_001026835
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FSETTTMKC 9
           FSE ++++C
Sbjct: 322 FSEKSSLRC 330
>NP_061825
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   FSETTTMKC 9
           F ET   KC
Sbjct: 115 FGETEEQKC 123
>NP_001245283
          Length = 962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   ETTTMKC 9
           ET+++KC
Sbjct: 658 ETSSLKC 664
>NP_004238
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   ETTTMKC 9
           ET+++KC
Sbjct: 668 ETSSLKC 674
>NP_001245282
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   ETTTMKC 9
           ET+++KC
Sbjct: 668 ETSSLKC 674
>NP_001136077
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   ETTTMKC 9
           ET+++KC
Sbjct: 633 ETSSLKC 639
>NP_001099003
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FSETTTM 7
           FSET+T+
Sbjct: 341 FSETSTL 347
>NP_443159
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   FSETTTMKC 9
           F E T +KC
Sbjct: 225 FQEGTEVKC 233
>NP_079189
          Length = 1026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FSETTTM 7
           FSET+T+
Sbjct: 341 FSETSTL 347
>NP_001253964
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   FSETTTMKC 9
           F E T +KC
Sbjct: 225 FQEGTEVKC 233
>XP_005257340
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   FSETTTMKC 9
           F ET   KC
Sbjct: 115 FGETEEQKC 123
>XP_006721919
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   FSETTTMKC 9
           F ET   KC
Sbjct: 115 FGETEEQKC 123
>XP_005257341
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   FSETTTMKC 9
           F ET   KC
Sbjct: 115 FGETEEQKC 123
>XP_005265867
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  FSETTTMKC 9
          F E T +KC
Sbjct: 8  FQEGTEVKC 16
>XP_005265868
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  FSETTTMKC 9
          F E T +KC
Sbjct: 8  FQEGTEVKC 16
>XP_005265869
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  FSETTTMKC 9
          F E T +KC
Sbjct: 8  FQEGTEVKC 16
>XP_005265866
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  FSETTTMKC 9
          F E T +KC
Sbjct: 8  FQEGTEVKC 16
>XP_005265865
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  FSETTTMKC 9
          F E T +KC
Sbjct: 8  FQEGTEVKC 16
>XP_005265864
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  FSETTTMKC 9
          F E T +KC
Sbjct: 8  FQEGTEVKC 16
>NP_001124336
          Length = 755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   FSETTTMKC 9
           F E T +KC
Sbjct: 225 FQEGTEVKC 233
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.116    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 468,064
Number of extensions: 963
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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