Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_775771                                                              20     390
NP_115927                                                              20     390
XP_006720466                                                           20     390
XP_005254230                                                           20     390
XP_006720465                                                           20     390
XP_005254228                                                           20     390
XP_006715292                                                           20     390
NP_001274523                                                           16    5631
NP_001274522                                                           16    5631
NP_001279                                                              16    5631
NP_004268                                                              16    5631
NP_001190981                                                           16    5631
NP_001190980                                                           16    5631
NP_002370                                                              16    5631
NP_660295                                                              16    5631
XP_006715337                                                           16    5631
XP_005249541                                                           16    5631
XP_005249540                                                           16    5631
NP_001243342                                                           15    9605
NP_001035196                                                           15    9605
NP_115745                                                              15    9605
NP_004808                                                              15    9605
NP_001164101                                                           15    9605
NP_963923                                                              15    9605
NP_005525                                                              15    9605
NP_001163886                                                           15    9605
NP_055455                                                              15    9605
NP_079461                                                              15    9605
NP_004409                                                              15    9605
NP_001154879                                                           15    9605
XP_005261026                                                           15    9605
XP_005257260                                                           15    9605
XP_005257258                                                           15    9605
XP_006721872                                                           15    9605
XP_006721871                                                           15    9605
XP_006721870                                                           15    9605
XP_006721869                                                           15    9605
XP_006719903                                                           15    9605
XP_006719703                                                           15    9605
XP_005254005                                                           15    9605
XP_005252372                                                           15    9605
XP_006717387                                                           15    9605
XP_005252371                                                           15    9605
XP_005252370                                                           15    9605
XP_005252369                                                           15    9605
XP_005252368                                                           15    9605
XP_005247035                                                           15    9605
XP_006712939                                                           15    9605
XP_006712938                                                           15    9605
XP_006712937                                                           15    9605
XP_006712936                                                           15    9605
XP_005247034                                                           15    9605
NP_061953                                                              15    9605
NP_001163885                                                           15    9605
NP_001163887                                                           15    9605
>NP_775771
          Length = 1244

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FRGMVRYAS 9
           FRG VRYAS
Sbjct: 186 FRGTVRYAS 194
>NP_115927
          Length = 1321

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FRGMVRYAS 9
           FRG VRYAS
Sbjct: 199 FRGTVRYAS 207
>XP_006720466
          Length = 1175

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FRGMVRYAS 9
           FRG VRYAS
Sbjct: 117 FRGTVRYAS 125
>XP_005254230
          Length = 1175

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FRGMVRYAS 9
           FRG VRYAS
Sbjct: 117 FRGTVRYAS 125
>XP_006720465
          Length = 1239

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FRGMVRYAS 9
           FRG VRYAS
Sbjct: 181 FRGTVRYAS 189
>XP_005254228
          Length = 1250

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FRGMVRYAS 9
           FRG VRYAS
Sbjct: 192 FRGTVRYAS 200
>XP_006715292
          Length = 759

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FRGMVRYAS 9
           FRG VRYAS
Sbjct: 186 FRGTVRYAS 194
>NP_001274523
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FRGMVRYAS 9
           FRG+ RY S
Sbjct: 203 FRGVHRYLS 211
>NP_001274522
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FRGMVRYAS 9
           FRG+ RY S
Sbjct: 203 FRGVHRYLS 211
>NP_001279
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FRGMVRYAS 9
           FRG+ RY S
Sbjct: 203 FRGVHRYLS 211
>NP_004268
          Length = 323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FRGMVRYA 8
          +RGMVR A
Sbjct: 68 YRGMVRTA 75
>NP_001190981
          Length = 245

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FRGMVRYA 8
          +RGMVR A
Sbjct: 68 YRGMVRTA 75
>NP_001190980
          Length = 300

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FRGMVRYA 8
          +RGMVR A
Sbjct: 68 YRGMVRTA 75
>NP_002370
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  GMVRYAS 9
          GMV YAS
Sbjct: 81 GMVNYAS 87
>NP_660295
          Length = 208

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RGMVRYAS 9
           RGMV Y S
Sbjct: 131 RGMVMYTS 138
>XP_006715337
          Length = 266

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FRGMVRYA 8
          +RGMVR A
Sbjct: 68 YRGMVRTA 75
>XP_005249541
          Length = 275

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FRGMVRYA 8
          +RGMVR A
Sbjct: 20 YRGMVRTA 27
>XP_005249540
          Length = 310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FRGMVRYA 8
          +RGMVR A
Sbjct: 68 YRGMVRTA 75
>NP_001243342
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FRGMVR 6
          FRGM+R
Sbjct: 68 FRGMLR 73
>NP_001035196
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FRGMVR 6
          FRGM+R
Sbjct: 88 FRGMLR 93
>NP_115745
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FRGMVR 6
          FRGM+R
Sbjct: 88 FRGMLR 93
>NP_004808
          Length = 1190

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 563 FRGLVR 568
>NP_001164101
          Length = 993

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 563 FRGLVR 568
>NP_963923
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 563 FRGLVR 568
>NP_005525
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 1   FRGMVRY 7
           +RG+++Y
Sbjct: 273 YRGLIKY 279
>NP_001163886
          Length = 1157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 567 FRGLVR 572
>NP_055455
          Length = 1199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GMVRYAS 9
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>NP_079461
          Length = 1990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FRGMVRYA 8
            +RG VRY+
Sbjct: 1705 YRGGVRYS 1712
>NP_004409
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   FRGMVRYAS 9
           F G+ RY S
Sbjct: 213 FEGLFRYKS 221
>NP_001154879
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   FRGMVRY 7
           FRG V Y
Sbjct: 288 FRGFVNY 294
>XP_005261026
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 1   FRGMVRY 7
           +RG+++Y
Sbjct: 292 YRGLIKY 298
>XP_005257260
          Length = 1990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FRGMVRYA 8
            +RG VRY+
Sbjct: 1705 YRGGVRYS 1712
>XP_005257258
          Length = 2000

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FRGMVRYA 8
            +RG VRY+
Sbjct: 1715 YRGGVRYS 1722
>XP_006721872
          Length = 2037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FRGMVRYA 8
            +RG VRY+
Sbjct: 1752 YRGGVRYS 1759
>XP_006721871
          Length = 2037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FRGMVRYA 8
            +RG VRY+
Sbjct: 1752 YRGGVRYS 1759
>XP_006721870
          Length = 2064

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FRGMVRYA 8
            +RG VRY+
Sbjct: 1779 YRGGVRYS 1786
>XP_006721869
          Length = 2074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FRGMVRYA 8
            +RG VRY+
Sbjct: 1789 YRGGVRYS 1796
>XP_006719903
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   FRGMVRY 7
           FRG V Y
Sbjct: 307 FRGFVNY 313
>XP_006719703
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FRGMVR 6
          FRGM+R
Sbjct: 88 FRGMLR 93
>XP_005254005
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FRGMVR 6
          FRGM+R
Sbjct: 88 FRGMLR 93
>XP_005252372
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 622 FRGLVR 627
>XP_006717387
          Length = 1188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 561 FRGLVR 566
>XP_005252371
          Length = 1194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 567 FRGLVR 572
>XP_005252370
          Length = 1212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 622 FRGLVR 627
>XP_005252369
          Length = 1213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 586 FRGLVR 591
>XP_005252368
          Length = 1249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 622 FRGLVR 627
>XP_005247035
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GMVRYAS 9
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_006712939
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GMVRYAS 9
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_006712938
          Length = 1138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GMVRYAS 9
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_006712937
          Length = 1199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GMVRYAS 9
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_006712936
          Length = 1199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GMVRYAS 9
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_005247034
          Length = 1199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GMVRYAS 9
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>NP_061953
          Length = 336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   FRGMVRY 7
           FRG V Y
Sbjct: 308 FRGFVNY 314
>NP_001163885
          Length = 1020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 540 FRGLVR 545
>NP_001163887
          Length = 1221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMVR 6
           FRG+VR
Sbjct: 594 FRGLVR 599
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.342    0.144    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 446,774
Number of extensions: 877
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package