Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055068                                                              17    3301
NP_001156750                                                           17    3301
NP_001156751                                                           17    3301
NP_009224                                                              17    4312
NP_001239133                                                           17    4312
NP_001002029                                                           17    4312
NP_001229752                                                           17    4312
NP_001258875                                                           16    5631
NP_003053                                                              16    5631
NP_644807                                                              16    5631
NP_003774                                                              16    5631
NP_004629                                                              16    7355
NP_542417                                                              16    7355
NP_003639                                                              16    7355
NP_690618                                                              16    7355
NP_001138899                                                           16    7355
NP_001096045                                                           16    7355
NP_443102                                                              16    7355
XP_006726175                                                           16    7355
XP_006726174                                                           16    7355
XP_006726087                                                           16    7355
XP_006726086                                                           16    7355
XP_006725985                                                           16    7355
XP_006725897                                                           16    7355
XP_006725896                                                           16    7355
XP_006725787                                                           16    7355
XP_006725786                                                           16    7355
XP_006725571                                                           16    7355
XP_006725570                                                           16    7355
XP_006715254                                                           16    7355
XP_005246167                                                           16    7355
XP_005246165                                                           16    7355
XP_005246164                                                           16    7355
NP_005088                                                              15    9605
NP_060606                                                              15    9605
NP_000179                                                              15    9605
NP_277033                                                              15    9605
NP_277032                                                              15    9605
NP_277035                                                              15    9605
NP_277031                                                              15    9605
NP_001245380                                                           15    9605
NP_872629                                                              15    9605
NP_001245379                                                           15    9605
XP_006721039                                                           15    9605
XP_005255732                                                           15    9605
XP_005270329                                                           15    9605
XP_005269794                                                           15    9605
XP_005269793                                                           15    9605
XP_005269792                                                           15    9605
XP_005266846                                                           15    9605
XP_005266845                                                           15    9605
XP_005266844                                                           15    9605
NP_004194                                                              15    9605
>NP_055068
          Length = 2725

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   RGMKSETS 9
           RGMKSE S
Sbjct: 126 RGMKSEHS 133
>NP_001156750
          Length = 2732

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   RGMKSETS 9
           RGMKSE S
Sbjct: 126 RGMKSEHS 133
>NP_001156751
          Length = 2731

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   RGMKSETS 9
           RGMKSE S
Sbjct: 126 RGMKSEHS 133
>NP_009224
          Length = 1744

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FRGMKSET 8
           FRG++S+T
Sbjct: 296 FRGLESQT 303
>NP_001239133
          Length = 1698

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FRGMKSET 8
           FRG++S+T
Sbjct: 296 FRGLESQT 303
>NP_001002029
          Length = 1744

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FRGMKSET 8
           FRG++S+T
Sbjct: 296 FRGLESQT 303
>NP_001229752
          Length = 1744

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FRGMKSET 8
           FRG++S+T
Sbjct: 296 FRGLESQT 303
>NP_001258875
          Length = 1530

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FRGMKSETS 9
           F+G+KS TS
Sbjct: 351 FQGLKSLTS 359
>NP_003053
          Length = 1523

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FRGMKSETS 9
           F+G+KS TS
Sbjct: 351 FQGLKSLTS 359
>NP_644807
          Length = 548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FRGMKSET 8
           FRG+KS T
Sbjct: 132 FRGLKSLT 139
>NP_003774
          Length = 422

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FRGMKSETS 9
          FR  KSET+
Sbjct: 67 FRSTKSETN 75
>NP_004629
          Length = 2157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 819 KGMRSET 825
>NP_542417
          Length = 2157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 819 KGMRSET 825
>NP_003639
          Length = 1170

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FRGMKSET 8
            FR ++SET
Sbjct: 1001 FRALRSET 1008
>NP_690618
          Length = 1214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FRGMKSET 8
            FR ++SET
Sbjct: 1045 FRALRSET 1052
>NP_001138899
          Length = 591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FRGMKSETS 9
           F+GMKS  S
Sbjct: 289 FKGMKSYIS 297
>NP_001096045
          Length = 624

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FRGMKSETS 9
           F+GMKS  S
Sbjct: 289 FKGMKSYIS 297
>NP_443102
          Length = 628

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FRGMKSETS 9
           F+GMKS  S
Sbjct: 289 FKGMKSYIS 297
>XP_006726175
          Length = 2086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 819 KGMRSET 825
>XP_006726174
          Length = 2156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 818 KGMRSET 824
>XP_006726087
          Length = 2086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 819 KGMRSET 825
>XP_006726086
          Length = 2156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 818 KGMRSET 824
>XP_006725985
          Length = 2156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 818 KGMRSET 824
>XP_006725897
          Length = 2086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 819 KGMRSET 825
>XP_006725896
          Length = 2156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 818 KGMRSET 824
>XP_006725787
          Length = 2086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 819 KGMRSET 825
>XP_006725786
          Length = 2156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 818 KGMRSET 824
>XP_006725571
          Length = 2086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 819 KGMRSET 825
>XP_006725570
          Length = 2156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 818 KGMRSET 824
>XP_006715254
          Length = 2086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RGMKSET 8
           +GM+SET
Sbjct: 819 KGMRSET 825
>XP_005246167
          Length = 680

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FRGMKSET 8
           FR ++SET
Sbjct: 511 FRALRSET 518
>XP_005246165
          Length = 1081

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FRGMKSET 8
           FR ++SET
Sbjct: 912 FRALRSET 919
>XP_005246164
          Length = 1102

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FRGMKSET 8
           FR ++SET
Sbjct: 933 FRALRSET 940
>NP_005088
          Length = 557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMKS 6
           FRG+KS
Sbjct: 135 FRGLKS 140
>NP_060606
          Length = 3477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    FRGMKS 6
            FRGMK+
Sbjct: 2395 FRGMKT 2400
>NP_000179
          Length = 917

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FRGMKSET 8
           FRG  SET
Sbjct: 768 FRGQISET 775
>NP_277033
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FRGMKSET 8
           FRG  SET
Sbjct: 772 FRGQISET 779
>NP_277032
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FRGMKSET 8
           FRG  SET
Sbjct: 772 FRGQISET 779
>NP_277035
          Length = 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FRGMKSET 8
           FRG  SET
Sbjct: 756 FRGQISET 763
>NP_277031
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FRGMKSET 8
           FRG  SET
Sbjct: 767 FRGQISET 774
>NP_001245380
          Length = 490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  FRGMKSET 8
          FRG  SET
Sbjct: 75 FRGEASET 82
>NP_872629
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  FRGMKSET 8
          FRG  SET
Sbjct: 84 FRGEASET 91
>NP_001245379
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  FRGMKSET 8
          FRG  SET
Sbjct: 15 FRGEASET 22
>XP_006721039
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  FRGMKSET 8
          FRG  SET
Sbjct: 84 FRGEASET 91
>XP_005255732
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FRGMKSET 8
           FRG  SET
Sbjct: 93  FRGEASET 100
>XP_005270329
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGMKS 6
           FRG+KS
Sbjct: 135 FRGLKS 140
>XP_005269794
          Length = 889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FRGMKSET 8
           FRG  SET
Sbjct: 740 FRGQISET 747
>XP_005269793
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FRGMKSET 8
           FRG  SET
Sbjct: 772 FRGQISET 779
>XP_005269792
          Length = 960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FRGMKSET 8
           FRG  SET
Sbjct: 811 FRGQISET 818
>XP_005266846
          Length = 135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FRGMKSE 7
          +RGMK+E
Sbjct: 8  WRGMKNE 14
>XP_005266845
          Length = 170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FRGMKSE 7
          +RGMK+E
Sbjct: 43 WRGMKNE 49
>XP_005266844
          Length = 179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FRGMKSE 7
          +RGMK+E
Sbjct: 52 WRGMKNE 58
>NP_004194
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  FRGMKSET 8
          FRG  SET
Sbjct: 84 FRGEASET 91
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.120    0.312 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 439,747
Number of extensions: 737
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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