Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              18    1482
XP_005266703                                                           18    1482
NP_003271                                                              16    5631
NP_001714                                                              16    7355
XP_005249383                                                           16    7355
NP_003310                                                              15    9605
NP_597676                                                              15    9605
NP_001243779                                                           15    9605
NP_596869                                                              15    9605
NP_001073276                                                           15    9605
NP_001073275                                                           15    9605
NP_055039                                                              15    9605
NP_036434                                                              15    9605
XP_005276461                                                           15    9605
XP_006722424                                                           15    9605
XP_005258217                                                           15    9605
XP_005246888                                                           15    9605
XP_006712788                                                           15    9605
XP_006712787                                                           15    9605
XP_006712786                                                           15    9605
XP_005270731                                                           15    9605
XP_005270730                                                           15    9605
XP_006710560                                                           15    9605
XP_006710559                                                           15    9605
XP_006710558                                                           15    9605
XP_006710557                                                           15    9605
XP_006710556                                                           15    9605
XP_006710555                                                           15    9605
XP_005270727                                                           15    9605
XP_006710554                                                           15    9605
XP_005270725                                                           15    9605
XP_005270724                                                           15    9605
XP_006710553                                                           15    9605
XP_005270723                                                           15    9605
XP_006710552                                                           15    9605
XP_006710551                                                           15    9605
XP_006710550                                                           15    9605
XP_006710549                                                           15    9605
XP_006710548                                                           15    9605
NP_001254479                                                           15    9605
NP_597681                                                              15    9605
>NP_443179
          Length = 2170

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    FQATRET 7
            FQATRET
Sbjct: 999  FQATRET 1005
>XP_005266703
          Length = 1501

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   FQATRET 7
           FQATRET
Sbjct: 330 FQATRET 336
>NP_003271
          Length = 161

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QATRETVT 9
           QAT ET+T
Sbjct: 122 QATGETIT 129
>NP_001714
          Length = 2649

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    FQATRETVT 9
            FQ  R TVT
Sbjct: 2089 FQGLRHTVT 2097
>XP_005249383
          Length = 3186

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    FQATRETVT 9
            FQ  R TVT
Sbjct: 2626 FQGLRHTVT 2634
>NP_003310
          Length = 26926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4     TRETVT 9
             TRET+T
Sbjct: 21370 TRETIT 21375
>NP_597676
          Length = 27051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4     TRETVT 9
             TRET+T
Sbjct: 21495 TRETIT 21500
>NP_001243779
          Length = 34350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4     TRETVT 9
             TRET+T
Sbjct: 28794 TRETIT 28799
>NP_596869
          Length = 33423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4     TRETVT 9
             TRET+T
Sbjct: 27867 TRETIT 27872
>NP_001073276
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FQATRETVT 9
           +Q+T ETV+
Sbjct: 126 YQSTSETVS 134
>NP_001073275
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FQATRETVT 9
           +Q+T ETV+
Sbjct: 126 YQSTSETVS 134
>NP_055039
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FQATRETVT 9
           +Q+T ETV+
Sbjct: 126 YQSTSETVS 134
>NP_036434
          Length = 1403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 298 FEATWETV 305
>XP_005276461
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FQATRETVT 9
           +Q+T ETV+
Sbjct: 126 YQSTSETVS 134
>XP_006722424
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FQATRETVT 9
           ++ATR TVT
Sbjct: 461 WEATRLTVT 469
>XP_005258217
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FQATRETVT 9
           ++ATR TVT
Sbjct: 461 WEATRLTVT 469
>XP_005246888
          Length = 22663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4     TRETVT 9
             TRET+T
Sbjct: 17107 TRETIT 17112
>XP_006712788
          Length = 24022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4     TRETVT 9
             TRET+T
Sbjct: 18466 TRETIT 18471
>XP_006712787
          Length = 33978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4     TRETVT 9
             TRET+T
Sbjct: 28422 TRETIT 28427
>XP_006712786
          Length = 35484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4     TRETVT 9
             TRET+T
Sbjct: 29928 TRETIT 29933
>XP_005270731
          Length = 1177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 298 FEATWETV 305
>XP_005270730
          Length = 1177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 298 FEATWETV 305
>XP_006710560
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_006710559
          Length = 1188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_006710558
          Length = 1191

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_006710557
          Length = 1191

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_006710556
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_006710555
          Length = 1403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 298 FEATWETV 305
>XP_005270727
          Length = 1403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 298 FEATWETV 305
>XP_006710554
          Length = 1421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_005270725
          Length = 1446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 298 FEATWETV 305
>XP_005270724
          Length = 1446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 298 FEATWETV 305
>XP_006710553
          Length = 1451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_005270723
          Length = 1459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 298 FEATWETV 305
>XP_006710552
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_006710551
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_006710550
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_006710549
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>XP_006710548
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FQATRETV 8
           F+AT ETV
Sbjct: 303 FEATWETV 310
>NP_001254479
          Length = 35991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4     TRETVT 9
             TRET+T
Sbjct: 30435 TRETIT 30440
>NP_597681
          Length = 27118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4     TRETVT 9
             TRET+T
Sbjct: 21562 TRETIT 21567
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.118    0.301 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 316,167
Number of extensions: 514
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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