Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              20     390
XP_006714104                                                           20     390
XP_005265800                                                           20     390
XP_006714103                                                           20     390
XP_006714102                                                           20     390
NP_001139129                                                           16    5631
NP_001139130                                                           16    5631
NP_002876                                                              16    5631
XP_006710870                                                           16    5631
XP_005246013                                                           16    5631
XP_006710869                                                           16    5631
XP_005246012                                                           16    5631
XP_005246011                                                           16    5631
XP_006710868                                                           16    5631
XP_006710867                                                           16    5631
NP_001077415                                                           16    7355
NP_055894                                                              16    7355
NP_653173                                                              16    7355
NP_065728                                                              16    7355
XP_006725302                                                           16    7355
XP_006725301                                                           16    7355
XP_006725300                                                           16    7355
XP_006725299                                                           16    7355
XP_006720969                                                           16    7355
XP_006720968                                                           16    7355
XP_005255504                                                           16    7355
XP_005255503                                                           16    7355
XP_005264468                                                           16    7355
XP_005264467                                                           16    7355
NP_653337                                                              16    7355
NP_066568                                                              15    9605
NP_001139470                                                           15    9605
XP_006713799                                                           15    9605
XP_005247779                                                           15    9605
>NP_006197
          Length = 1089

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FNFYALKAT 9
           FN YALKAT
Sbjct: 203 FNVYALKAT 211
>XP_006714104
          Length = 832

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FNFYALKAT 9
           FN YALKAT
Sbjct: 228 FNVYALKAT 236
>XP_005265800
          Length = 1089

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FNFYALKAT 9
           FN YALKAT
Sbjct: 203 FNVYALKAT 211
>XP_006714103
          Length = 1111

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FNFYALKAT 9
           FN YALKAT
Sbjct: 225 FNVYALKAT 233
>XP_006714102
          Length = 1114

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FNFYALKAT 9
           FN YALKAT
Sbjct: 228 FNVYALKAT 236
>NP_001139129
          Length = 681

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FNFYALKA 8
           FN+Y+L A
Sbjct: 100 FNYYSLDA 107
>NP_001139130
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FNFYALKA 8
           FN+Y+L A
Sbjct: 164 FNYYSLDA 171
>NP_002876
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FNFYALKA 8
           FN+Y+L A
Sbjct: 100 FNYYSLDA 107
>XP_006710870
          Length = 779

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FNFYALKA 8
           FN+Y+L A
Sbjct: 131 FNYYSLDA 138
>XP_005246013
          Length = 800

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FNFYALKA 8
           FN+Y+L A
Sbjct: 237 FNYYSLDA 244
>XP_006710869
          Length = 812

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FNFYALKA 8
           FN+Y+L A
Sbjct: 164 FNYYSLDA 171
>XP_005246012
          Length = 818

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FNFYALKA 8
           FN+Y+L A
Sbjct: 237 FNYYSLDA 244
>XP_005246011
          Length = 826

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FNFYALKA 8
           FN+Y+L A
Sbjct: 237 FNYYSLDA 244
>XP_006710868
          Length = 877

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FNFYALKA 8
           FN+Y+L A
Sbjct: 237 FNYYSLDA 244
>XP_006710867
          Length = 885

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FNFYALKA 8
           FN+Y+L A
Sbjct: 237 FNYYSLDA 244
>NP_001077415
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FNFYAL 6
           FNF+AL
Sbjct: 304 FNFFAL 309
>NP_055894
          Length = 914

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFYALK 7
           NFY+LK
Sbjct: 216 NFYSLK 221
>NP_653173
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NFYALK 7
           NFY+LK
Sbjct: 216 NFYSLK 221
>NP_065728
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FNFYALK 7
           F FYAL+
Sbjct: 261 FRFYALR 267
>XP_006725302
          Length = 235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FNFYALK 7
           F FYAL+
Sbjct: 197 FRFYALR 203
>XP_006725301
          Length = 236

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FNFYALK 7
           F FYAL+
Sbjct: 198 FRFYALR 204
>XP_006725300
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FNFYALK 7
           F FYAL+
Sbjct: 261 FRFYALR 267
>XP_006725299
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FNFYALK 7
           F FYAL+
Sbjct: 261 FRFYALR 267
>XP_006720969
          Length = 235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FNFYALK 7
           F FYAL+
Sbjct: 197 FRFYALR 203
>XP_006720968
          Length = 236

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FNFYALK 7
           F FYAL+
Sbjct: 198 FRFYALR 204
>XP_005255504
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FNFYALK 7
           F FYAL+
Sbjct: 261 FRFYALR 267
>XP_005255503
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FNFYALK 7
           F FYAL+
Sbjct: 261 FRFYALR 267
>XP_005264468
          Length = 370

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FNFYAL 6
           FNF+AL
Sbjct: 331 FNFFAL 336
>XP_005264467
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FNFYAL 6
           FNF+AL
Sbjct: 360 FNFFAL 365
>NP_653337
          Length = 441

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FNFYAL 6
           FNF+AL
Sbjct: 402 FNFFAL 407
>NP_066568
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  FNFYALKA 8
          F++Y +KA
Sbjct: 58 FSYYGMKA 65
>NP_001139470
          Length = 698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  FNFYALKA 8
          F++Y +KA
Sbjct: 58 FSYYGMKA 65
>XP_006713799
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  FNFYALKA 8
          F++Y +KA
Sbjct: 58 FSYYGMKA 65
>XP_005247779
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  FNFYALKA 8
          F++Y +KA
Sbjct: 58 FSYYGMKA 65
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.140    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 383,294
Number of extensions: 339
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package