Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444254                                                              20     299
NP_444253                                                              20     299
XP_005247551                                                           20     299
XP_005247550                                                           20     299
XP_006713712                                                           20     299
XP_005247549                                                           20     299
NP_001035247                                                           17    3301
NP_001138786                                                           17    3301
XP_005263307                                                           17    3301
XP_006714391                                                           17    3301
XP_006714390                                                           17    3301
NP_996879                                                              17    4312
NP_004612                                                              17    4312
NP_001139                                                              17    4312
NP_663160                                                              17    4312
NP_663162                                                              17    4312
NP_056365                                                              17    4312
NP_663161                                                              17    4312
NP_004626                                                              17    4312
NP_001230854                                                           17    4312
NP_078887                                                              17    4312
NP_919226                                                              17    4312
NP_001263314                                                           17    4312
NP_001018099                                                           17    4312
NP_001230855                                                           17    4312
NP_066966                                                              17    4312
NP_001190179                                                           17    4312
XP_005254720                                                           17    4312
XP_005254719                                                           17    4312
XP_005254718                                                           17    4312
XP_006718961                                                           17    4312
XP_006715747                                                           17    4312
XP_006715746                                                           17    4312
XP_006715745                                                           17    4312
XP_006715744                                                           17    4312
XP_005249755                                                           17    4312
XP_006714257                                                           17    4312
XP_005263002                                                           17    4312
XP_005262999                                                           17    4312
XP_006714252                                                           17    4312
XP_006714251                                                           17    4312
XP_006714250                                                           17    4312
XP_006710493                                                           17    4312
XP_006710491                                                           17    4312
XP_006710490                                                           17    4312
XP_006710489                                                           17    4312
NP_060229                                                              16    5631
NP_149109                                                              16    7355
NP_003961                                                              16    7355
NP_075378                                                              16    7355
NP_055524                                                              16    7355
NP_065789                                                              16    7355
NP_004668                                                              16    7355
XP_006716300                                                           16    7355
XP_006714558                                                           16    7355
NP_001002906                                                           16    7355
NP_006222                                                              15    9605
NP_006759                                                              15    9605
NP_036292                                                              15    9605
NP_001265645                                                           15    9605
NP_068734                                                              15    9605
NP_001184225                                                           15    9605
NP_001184224                                                           15    9605
XP_005260117                                                           15    9605
XP_006722938                                                           15    9605
XP_006722937                                                           15    9605
XP_005260116                                                           15    9605
XP_005260115                                                           15    9605
XP_006722936                                                           15    9605
XP_005259788                                                           15    9605
XP_005254001                                                           15    9605
XP_005266987                                                           15    9605
XP_006715521                                                           15    9605
NP_001384                                                              15    9605
NP_689570                                                              15    9605
>NP_444254
          Length = 1845

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FNEISDDAK 9
            F+EISDDAK
Sbjct: 1616 FDEISDDAK 1624
>NP_444253
          Length = 1914

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FNEISDDAK 9
            F+EISDDAK
Sbjct: 1685 FDEISDDAK 1693
>XP_005247551
          Length = 1855

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FNEISDDAK 9
            F+EISDDAK
Sbjct: 1626 FDEISDDAK 1634
>XP_005247550
          Length = 1856

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FNEISDDAK 9
            F+EISDDAK
Sbjct: 1627 FDEISDDAK 1635
>XP_006713712
          Length = 1923

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FNEISDDAK 9
            F+EISDDAK
Sbjct: 1695 FDEISDDAK 1703
>XP_005247549
          Length = 1924

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FNEISDDAK 9
            F+EISDDAK
Sbjct: 1695 FDEISDDAK 1703
>NP_001035247
          Length = 390

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FNEISDD 7
           FNE+S+D
Sbjct: 302 FNEVSED 308
>NP_001138786
          Length = 399

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FNEISDD 7
           FNE+S+D
Sbjct: 302 FNEVSED 308
>XP_005263307
          Length = 363

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FNEISDD 7
           FNE+S+D
Sbjct: 302 FNEVSED 308
>XP_006714391
          Length = 390

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FNEISDD 7
           FNE+S+D
Sbjct: 302 FNEVSED 308
>XP_006714390
          Length = 390

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FNEISDD 7
           FNE+S+D
Sbjct: 302 FNEVSED 308
>NP_996879
          Length = 2223

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           NEI DDA
Sbjct: 742 NEIKDDA 748
>NP_004612
          Length = 931

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FNEISDDA 8
           F EI DDA
Sbjct: 731 FQEIEDDA 738
>NP_001139
          Length = 3957

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FNEISDDA 8
            F EISD+A
Sbjct: 3478 FEEISDEA 3485
>NP_663160
          Length = 856

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NEISDD 7
           NEISDD
Sbjct: 435 NEISDD 440
>NP_663162
          Length = 820

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NEISDD 7
           NEISDD
Sbjct: 399 NEISDD 404
>NP_056365
          Length = 887

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NEISDD 7
           NEISDD
Sbjct: 466 NEISDD 471
>NP_663161
          Length = 851

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NEISDD 7
           NEISDD
Sbjct: 430 NEISDD 435
>NP_004626
          Length = 382

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   FNEISDDAK 9
           ++E+S+DAK
Sbjct: 270 WSEVSEDAK 278
>NP_001230854
          Length = 382

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   FNEISDDAK 9
           ++E+S+DAK
Sbjct: 270 WSEVSEDAK 278
>NP_078887
          Length = 982

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FNEISDDAK 9
           +N +SDDA+
Sbjct: 164 YNMLSDDAR 172
>NP_919226
          Length = 1209

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FNEISDDA 8
           F+E SDDA
Sbjct: 233 FSEYSDDA 240
>NP_001263314
          Length = 383

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FNEISDDAK 9
           +N +SDDA+
Sbjct: 164 YNMLSDDAR 172
>NP_001018099
          Length = 845

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FNEISDDAK 9
          +N +SDDA+
Sbjct: 27 YNMLSDDAR 35
>NP_001230855
          Length = 382

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   FNEISDDAK 9
           ++E+S+DAK
Sbjct: 270 WSEVSEDAK 278
>NP_066966
          Length = 532

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FNEISDD 7
          FN+I+DD
Sbjct: 86 FNQIADD 92
>NP_001190179
          Length = 529

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FNEISDD 7
          FN+I+DD
Sbjct: 83 FNQIADD 89
>XP_005254720
          Length = 772

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FNEISDDAK 9
           +N +SDDA+
Sbjct: 164 YNMLSDDAR 172
>XP_005254719
          Length = 845

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FNEISDDAK 9
          +N +SDDA+
Sbjct: 27 YNMLSDDAR 35
>XP_005254718
          Length = 982

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FNEISDDAK 9
           +N +SDDA+
Sbjct: 164 YNMLSDDAR 172
>XP_006718961
          Length = 906

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FNEISDDA 8
           F EI DDA
Sbjct: 731 FQEIEDDA 738
>XP_006715747
          Length = 534

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NEISDD 7
           NEISDD
Sbjct: 113 NEISDD 118
>XP_006715746
          Length = 820

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NEISDD 7
           NEISDD
Sbjct: 399 NEISDD 404
>XP_006715745
          Length = 851

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NEISDD 7
           NEISDD
Sbjct: 430 NEISDD 435
>XP_006715744
          Length = 856

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NEISDD 7
           NEISDD
Sbjct: 435 NEISDD 440
>XP_005249755
          Length = 887

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NEISDD 7
           NEISDD
Sbjct: 466 NEISDD 471
>XP_006714257
          Length = 4145

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FNEISDDA 8
            F EISD+A
Sbjct: 3487 FEEISDEA 3494
>XP_005263002
          Length = 4072

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FNEISDDA 8
            F EISD+A
Sbjct: 3445 FEEISDEA 3452
>XP_005262999
          Length = 4147

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FNEISDDA 8
            F EISD+A
Sbjct: 3489 FEEISDEA 3496
>XP_006714252
          Length = 4153

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FNEISDDA 8
            F EISD+A
Sbjct: 3495 FEEISDEA 3502
>XP_006714251
          Length = 4165

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FNEISDDA 8
            F EISD+A
Sbjct: 3507 FEEISDEA 3514
>XP_006714250
          Length = 4190

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FNEISDDA 8
            F EISD+A
Sbjct: 3532 FEEISDEA 3539
>XP_006710493
          Length = 1305

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           NEI DDA
Sbjct: 742 NEIKDDA 748
>XP_006710491
          Length = 1496

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  NEISDDA 8
          NEI DDA
Sbjct: 15 NEIKDDA 21
>XP_006710490
          Length = 2158

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           NEI DDA
Sbjct: 677 NEIKDDA 683
>XP_006710489
          Length = 2185

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           NEI DDA
Sbjct: 742 NEIKDDA 748
>NP_060229
          Length = 1027

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FNEISDDAK 9
           FNE  DD K
Sbjct: 153 FNEEDDDLK 161
>NP_149109
          Length = 596

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FNEISDDAK 9
           F  +SD+AK
Sbjct: 506 FEAVSDEAK 514
>NP_003961
          Length = 1465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FNEISDDAK 9
           + EISDD +
Sbjct: 947 YEEISDDER 955
>NP_075378
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           NE+ DDA
Sbjct: 147 NELEDDA 153
>NP_055524
          Length = 3546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FNEISDDAK 9
            F E+SD +K
Sbjct: 2133 FKEVSDHSK 2141
>NP_065789
          Length = 1771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    NEISDDAK 9
            NE++DD++
Sbjct: 1601 NEVADDSQ 1608
>NP_004668
          Length = 117

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  FNEISDD 7
          FN I+DD
Sbjct: 4  FNSIADD 10
>XP_006716300
          Length = 1445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FNEISDDAK 9
           + EISDD +
Sbjct: 947 YEEISDDER 955
>XP_006714558
          Length = 2612

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  FNEISDDAK 9
          F E+S D K
Sbjct: 13 FQEVSQDGK 21
>NP_001002906
          Length = 117

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  FNEISDD 7
          FN I+DD
Sbjct: 4  FNSIADD 10
>NP_006222
          Length = 2286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FNEISDD 7
           FN+++DD
Sbjct: 524 FNKLTDD 530
>NP_006759
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   FNEISDD 7
           FN I DD
Sbjct: 226 FNSIEDD 232
>NP_036292
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           N+I DDA
Sbjct: 244 NDIEDDA 250
>NP_001265645
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           N+I DDA
Sbjct: 244 NDIEDDA 250
>NP_068734
          Length = 149

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  NEISDDA 8
          NEI DD+
Sbjct: 78 NEIKDDS 84
>NP_001184225
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FNEISDD 7
           FN+++DD
Sbjct: 568 FNKLADD 574
>NP_001184224
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FNEISDD 7
           FN+++DD
Sbjct: 568 FNKLADD 574
>XP_005260117
          Length = 163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  NEISDDA 8
          NEI DD+
Sbjct: 92 NEIKDDS 98
>XP_006722938
          Length = 166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           NEI DD+
Sbjct: 95  NEIKDDS 101
>XP_006722937
          Length = 166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           NEI DD+
Sbjct: 95  NEIKDDS 101
>XP_005260116
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  NEISDDA 8
          NEI DD+
Sbjct: 78 NEIKDDS 84
>XP_005260115
          Length = 566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  NEISDDA 8
          NEI DD+
Sbjct: 92 NEIKDDS 98
>XP_006722936
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           NEI DD+
Sbjct: 95  NEIKDDS 101
>XP_005259788
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  NEISDDA 8
          NEI DD+
Sbjct: 75 NEIKDDS 81
>XP_005254001
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   FNEISDD 7
           FN I DD
Sbjct: 267 FNSIEDD 273
>XP_005266987
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           N+I DDA
Sbjct: 244 NDIEDDA 250
>XP_006715521
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NEISDDA 8
           N+I DDA
Sbjct: 244 NDIEDDA 250
>NP_001384
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FNEISDD 7
           FN+++DD
Sbjct: 590 FNKLADD 596
>NP_689570
          Length = 595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  NEISDDA 8
          NEI DD+
Sbjct: 72 NEIKDDS 78
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.128    0.336 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 605,815
Number of extensions: 1316
Number of successful extensions: 75
Number of sequences better than 10000.0: 75
Number of HSP's gapped: 75
Number of HSP's successfully gapped: 75
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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