Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002542                                                              17    4312
NP_940931                                                              17    4312
NP_036505                                                              17    4312
XP_006721943                                                           17    4312
XP_006721942                                                           17    4312
XP_005268211                                                           17    4312
NP_001092872                                                           17    4312
NP_001185951                                                           16    5631
NP_055526                                                              16    5631
XP_006721168                                                           16    5631
XP_005255778                                                           16    5631
XP_005255776                                                           16    5631
NP_001229510                                                           16    7355
NP_061866                                                              16    7355
NP_056372                                                              16    7355
XP_005260790                                                           16    7355
XP_005260787                                                           16    7355
XP_005260789                                                           16    7355
XP_006723640                                                           16    7355
XP_006723639                                                           16    7355
XP_005260788                                                           16    7355
XP_006723638                                                           16    7355
XP_006723637                                                           16    7355
XP_006723636                                                           16    7355
NP_958441                                                              15    9605
NP_958439                                                              15    9605
NP_958440                                                              15    9605
NP_005219                                                              15    9605
NP_055615                                                              15    9605
NP_001193411                                                           15    9605
NP_001193409                                                           15    9605
XP_005249976                                                           15    9605
XP_005249977                                                           15    9605
XP_006715868                                                           15    9605

>NP_002542
          Length = 321

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2 MSLQKMMK 9
         MSLQK+M+
Sbjct: 1 MSLQKLME 8



>NP_940931
          Length = 1503

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    FMSLQKMMK 9
            +  LQKMMK
Sbjct: 1281 YKDLQKMMK 1289



>NP_036505
          Length = 321

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2 MSLQKMMK 9
         MSLQK+M+
Sbjct: 1 MSLQKLME 8



>XP_006721943
          Length = 1456

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    FMSLQKMMK 9
            +  LQKMMK
Sbjct: 1281 YKDLQKMMK 1289



>XP_006721942
          Length = 1503

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    FMSLQKMMK 9
            +  LQKMMK
Sbjct: 1281 YKDLQKMMK 1289



>XP_005268211
          Length = 580

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FMSLQKMMK 9
           + SLQ MMK
Sbjct: 444 YQSLQSMMK 452



>NP_001092872
          Length = 580

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FMSLQKMMK 9
           + SLQ MMK
Sbjct: 444 YQSLQSMMK 452



>NP_001185951
          Length = 1012

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MSLQKMMK 9
           MSLQ +MK
Sbjct: 226 MSLQNLMK 233



>NP_055526
          Length = 991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MSLQKMMK 9
           MSLQ +MK
Sbjct: 226 MSLQNLMK 233



>XP_006721168
          Length = 971

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MSLQKMMK 9
           MSLQ +MK
Sbjct: 226 MSLQNLMK 233



>XP_005255778
          Length = 991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MSLQKMMK 9
           MSLQ +MK
Sbjct: 226 MSLQNLMK 233



>XP_005255776
          Length = 1012

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MSLQKMMK 9
           MSLQ +MK
Sbjct: 226 MSLQNLMK 233



>NP_001229510
          Length = 944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 707 FMTMQKM 713



>NP_061866
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 658 FMTMQKM 664



>NP_056372
          Length = 1954

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MSLQKMMK 9
            M LQKM+K
Sbjct: 1027 MLLQKMLK 1034



>XP_005260790
          Length = 689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 452 FMTMQKM 458



>XP_005260787
          Length = 861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 624 FMTMQKM 630



>XP_005260789
          Length = 850

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 613 FMTMQKM 619



>XP_006723640
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_006723639
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_005260788
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_006723638
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_006723637
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_006723636
          Length = 944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FMSLQKM 7
           FM++QKM
Sbjct: 707 FMTMQKM 713



>NP_958441
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FMSLQKM 7
          F+SLQ+M
Sbjct: 48 FLSLQRM 54



>NP_958439
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FMSLQKM 7
          F+SLQ+M
Sbjct: 48 FLSLQRM 54



>NP_958440
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FMSLQKM 7
          F+SLQ+M
Sbjct: 48 FLSLQRM 54



>NP_005219
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FMSLQKM 7
          F+SLQ+M
Sbjct: 48 FLSLQRM 54



>NP_055615
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FMSLQKMMK 9
           +  LQK+MK
Sbjct: 140 YQKLQKIMK 148



>NP_001193411
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FMSLQKMMK 9
           +  LQK+MK
Sbjct: 140 YQKLQKIMK 148



>NP_001193409
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FMSLQKMMK 9
           +  LQK+MK
Sbjct: 140 YQKLQKIMK 148



>XP_005249976
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FMSLQKMMK 9
           +  LQK+MK
Sbjct: 140 YQKLQKIMK 148



>XP_005249977
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FMSLQKMMK 9
           +  LQK+MK
Sbjct: 140 YQKLQKIMK 148



>XP_006715868
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FMSLQKMMK 9
           +  LQK+MK
Sbjct: 140 YQKLQKIMK 148



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.341    0.131    0.332 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 293,345
Number of extensions: 344
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)