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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003290                                                              19     665
NP_031371                                                              17    3301
XP_005266819                                                           17    3301
XP_006722604                                                           17    3301
NP_001120676                                                           16    5631
NP_001273200                                                           16    5631
NP_001273197                                                           16    5631
NP_001273196                                                           16    5631
NP_001273198                                                           16    5631
NP_001273199                                                           16    5631
NP_001120678                                                           16    5631
NP_001120677                                                           16    5631
NP_002125                                                              16    5631
NP_001028252                                                           16    7355
NP_055441                                                              16    7355
NP_443199                                                              16    7355
NP_001166028                                                           15    9605
NP_113584                                                              15    9605
NP_001129663                                                           15    9605
NP_056308                                                              15    9605
NP_005886                                                              15    9605
NP_001034663                                                           15    9605
XP_005262029                                                           15    9605
XP_005262028                                                           15    9605
XP_005262026                                                           15    9605
XP_006724637                                                           15    9605
XP_005262025                                                           15    9605
XP_005262024                                                           15    9605
XP_005262023                                                           15    9605
XP_005262022                                                           15    9605
XP_006719801                                                           15    9605
XP_006719800                                                           15    9605
XP_006719799                                                           15    9605
XP_005266219                                                           15    9605
XP_006710690                                                           15    9605

>NP_003290
          Length = 803

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FMAGSIRKE 9
           FMAGS RKE
Sbjct: 540 FMAGSSRKE 548



>NP_031371
          Length = 1845

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FMAGSIRKE 9
           F+ GSIR+E
Sbjct: 468 FLPGSIREE 476



>XP_005266819
          Length = 1790

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FMAGSIRKE 9
           F+ GSIR+E
Sbjct: 413 FLPGSIREE 421



>XP_006722604
          Length = 1847

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FMAGSIRKE 9
           F+ GSIR+E
Sbjct: 470 FLPGSIREE 478



>NP_001120676
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FMAGSIRKE 9
          F+ G+I+KE
Sbjct: 57 FLKGNIKKE 65



>NP_001273200
          Length = 287

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FMAGSIRKE 9
          F+ G+I+KE
Sbjct: 28 FLKGNIKKE 36



>NP_001273197
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FMAGSIRKE 9
          F+ G+I+KE
Sbjct: 57 FLKGNIKKE 65



>NP_001273196
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FMAGSIRKE 9
           F+ G+I+KE
Sbjct: 111 FLKGNIKKE 119



>NP_001273198
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FMAGSIRKE 9
          F+ G+I+KE
Sbjct: 57 FLKGNIKKE 65



>NP_001273199
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FMAGSIRKE 9
          F+ G+I+KE
Sbjct: 57 FLKGNIKKE 65



>NP_001120678
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FMAGSIRKE 9
          F+ G+I+KE
Sbjct: 57 FLKGNIKKE 65



>NP_001120677
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FMAGSIRKE 9
          F+ G+I+KE
Sbjct: 57 FLKGNIKKE 65



>NP_002125
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FMAGSIRKE 9
          F+ G+I+KE
Sbjct: 57 FLKGNIKKE 65



>NP_001028252
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  FMAGSI 6
          FMAGSI
Sbjct: 49 FMAGSI 54



>NP_055441
          Length = 306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1 FMAGSI 6
         FMAGSI
Sbjct: 4 FMAGSI 9



>NP_443199
          Length = 378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FMAGSIRKE 9
           F  GS+RK+
Sbjct: 315 FFVGSLRKK 323



>NP_001166028
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GSIRKE 9
           GS+RKE
Sbjct: 114 GSVRKE 119



>NP_113584
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MAGSIRKE 9
            M G+IRK+
Sbjct: 4215 MTGAIRKQ 4222



>NP_001129663
          Length = 851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MAGSIRK 8
           MAG++RK
Sbjct: 192 MAGALRK 198



>NP_056308
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MAGSIRK 8
           MAG++RK
Sbjct: 192 MAGALRK 198



>NP_005886
          Length = 1498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GSIRKE 9
           GS+RKE
Sbjct: 114 GSVRKE 119



>NP_001034663
          Length = 626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GSIRKE 9
           GS+RKE
Sbjct: 602 GSVRKE 607



>XP_005262029
          Length = 4240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MAGSIRKE 9
            M G+IRK+
Sbjct: 4081 MTGAIRKQ 4088



>XP_005262028
          Length = 4341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MAGSIRKE 9
            M G+IRK+
Sbjct: 4182 MTGAIRKQ 4189



>XP_005262026
          Length = 4359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MAGSIRKE 9
            M G+IRK+
Sbjct: 4200 MTGAIRKQ 4207



>XP_006724637
          Length = 4365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MAGSIRKE 9
            M G+IRK+
Sbjct: 4206 MTGAIRKQ 4213



>XP_005262025
          Length = 4373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MAGSIRKE 9
            M G+IRK+
Sbjct: 4214 MTGAIRKQ 4221



>XP_005262024
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MAGSIRKE 9
            M G+IRK+
Sbjct: 4215 MTGAIRKQ 4222



>XP_005262023
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MAGSIRKE 9
            M G+IRK+
Sbjct: 4215 MTGAIRKQ 4222



>XP_005262022
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MAGSIRKE 9
            M G+IRK+
Sbjct: 4215 MTGAIRKQ 4222



>XP_006719801
          Length = 1498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GSIRKE 9
           GS+RKE
Sbjct: 114 GSVRKE 119



>XP_006719800
          Length = 1498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GSIRKE 9
           GS+RKE
Sbjct: 114 GSVRKE 119



>XP_006719799
          Length = 1498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GSIRKE 9
           GS+RKE
Sbjct: 114 GSVRKE 119



>XP_005266219
          Length = 1498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GSIRKE 9
           GS+RKE
Sbjct: 114 GSVRKE 119



>XP_006710690
          Length = 624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GSIRKE 9
           GS+RKE
Sbjct: 602 GSVRKE 607



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.136    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 465,508
Number of extensions: 637
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)