Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_061836                                                              19     869
NP_387505                                                              19     869
XP_005261051                                                           19     869
NP_722576                                                              18    1935
NP_065861                                                              15    9605
NP_001879                                                              15    9605
NP_001070993                                                           15    9605
NP_001258604                                                           15    9605
NP_787036                                                              15    9605
NP_787034                                                              15    9605
NP_787033                                                              15    9605
NP_055642                                                              15    9605
NP_001014444                                                           15    9605
NP_001257620                                                           15    9605
NP_071930                                                              15    9605
XP_006723641                                                           15    9605
XP_005267973                                                           15    9605
XP_005265457                                                           15    9605
XP_006711736                                                           15    9605
XP_005245701                                                           15    9605
XP_005245700                                                           15    9605
NP_001070994                                                           15    9605
>NP_061836
          Length = 2320

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FLKIESSE 8
            FLKIESSE
Sbjct: 1750 FLKIESSE 1757
>NP_387505
          Length = 2269

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FLKIESSE 8
            FLKIESSE
Sbjct: 1750 FLKIESSE 1757
>XP_005261051
          Length = 2242

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FLKIESSE 8
            FLKIESSE
Sbjct: 1672 FLKIESSE 1679
>NP_722576
          Length = 3080

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FLKIESSEV 9
           FLK ESSEV
Sbjct: 163 FLKNESSEV 171
>NP_065861
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FLKIESSEV 9
          FLK+ES  +
Sbjct: 16 FLKLESHSI 24
>NP_001879
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   FLKIESSEV 9
           FLK  SSEV
Sbjct: 130 FLKPPSSEV 138
>NP_001070993
          Length = 650

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FLKIESSE 8
          FL+ ESSE
Sbjct: 91 FLEYESSE 98
>NP_001258604
          Length = 805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LKIESSE 8
           +K+ESSE
Sbjct: 139 MKVESSE 145
>NP_787036
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLKIESSE 8
           +LK+ES E
Sbjct: 201 YLKVESCE 208
>NP_787034
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLKIESSE 8
           +LK+ES E
Sbjct: 201 YLKVESCE 208
>NP_787033
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLKIESSE 8
           +LK+ES E
Sbjct: 201 YLKVESCE 208
>NP_055642
          Length = 810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LKIESSE 8
           +K+ESSE
Sbjct: 139 MKVESSE 145
>NP_001014444
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  FLKIESSEV 9
          FLK  SSEV
Sbjct: 88 FLKPPSSEV 96
>NP_001257620
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLKIESSE 8
           +LK+ES E
Sbjct: 201 YLKVESCE 208
>NP_071930
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FLKIESSE 8
          FL+ ESSE
Sbjct: 91 FLEYESSE 98
>XP_006723641
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLKIESSE 8
           +LK+ES E
Sbjct: 201 YLKVESCE 208
>XP_005267973
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FLKIESSEV 9
          FLK+ES  +
Sbjct: 44 FLKLESHSI 52
>XP_005265457
          Length = 625

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FLKIESSE 8
          FL+ ESSE
Sbjct: 91 FLEYESSE 98
>XP_006711736
          Length = 810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LKIESSE 8
           +K+ESSE
Sbjct: 139 MKVESSE 145
>XP_005245701
          Length = 810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LKIESSE 8
           +K+ESSE
Sbjct: 139 MKVESSE 145
>XP_005245700
          Length = 810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LKIESSE 8
           +K+ESSE
Sbjct: 139 MKVESSE 145
>NP_001070994
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FLKIESSE 8
          FL+ ESSE
Sbjct: 91 FLEYESSE 98
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.130    0.300 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 294,716
Number of extensions: 242
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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