Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              19     869
XP_006717990                                                           19     869
XP_006717989                                                           19     869
NP_001258779                                                           17    3301
NP_954863                                                              17    3301
NP_570141                                                              17    3301
NP_001135440                                                           17    3301
NP_659414                                                              17    4312
NP_001182144                                                           17    4312
NP_110413                                                              17    4312
NP_001155180                                                           17    4312
XP_005244990                                                           17    4312
XP_006711257                                                           17    4312
XP_005244989                                                           17    4312
XP_005244988                                                           17    4312
XP_006711256                                                           17    4312
XP_006711255                                                           17    4312
NP_653226                                                              16    7355
NP_001275949                                                           16    7355
NP_659464                                                              16    7355
NP_001264004                                                           16    7355
XP_005259370                                                           16    7355
XP_006723477                                                           16    7355
XP_005259369                                                           16    7355
XP_005259368                                                           16    7355
XP_005252887                                                           16    7355
XP_005252886                                                           16    7355
XP_005248961                                                           16    7355
XP_006711799                                                           16    7355
NP_055870                                                              16    7355
NP_000042                                                              15    9605
NP_001164564                                                           15    9605
NP_653236                                                              15    9605
XP_006725169                                                           15    9605
XP_005271621                                                           15    9605
XP_006718907                                                           15    9605
XP_006718906                                                           15    9605
XP_005271619                                                           15    9605
XP_005271618                                                           15    9605
XP_005266097                                                           15    9605
XP_005247172                                                           15    9605
XP_005247171                                                           15    9605
XP_005247170                                                           15    9605
>NP_000305
          Length = 403

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FLKAQEAL 8
           FLKAQEAL
Sbjct: 145 FLKAQEAL 152
>XP_006717990
          Length = 175

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FLKAQEAL 8
           FLKAQEAL
Sbjct: 130 FLKAQEAL 137
>XP_006717989
          Length = 388

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FLKAQEAL 8
           FLKAQEAL
Sbjct: 130 FLKAQEAL 137
>NP_001258779
          Length = 193

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  FLKAQEALY 9
          FL A+E+LY
Sbjct: 12 FLTAEESLY 20
>NP_954863
          Length = 522

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLKAQEALY 9
           FL A+E+LY
Sbjct: 341 FLTAEESLY 349
>NP_570141
          Length = 445

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLKAQEALY 9
           FL A+E+LY
Sbjct: 264 FLTAEESLY 272
>NP_001135440
          Length = 411

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLKAQEALY 9
           FL A+E+LY
Sbjct: 230 FLTAEESLY 238
>NP_659414
          Length = 426

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLKAQEALY 9
           FL+AQ AL+
Sbjct: 330 FLRAQAALF 338
>NP_001182144
          Length = 775

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLKAQEALY 9
           FL+AQ AL+
Sbjct: 330 FLRAQAALF 338
>NP_110413
          Length = 482

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KAQEALY 9
          KA+EALY
Sbjct: 78 KAEEALY 84
>NP_001155180
          Length = 476

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KAQEALY 9
          KA+EALY
Sbjct: 78 KAEEALY 84
>XP_005244990
          Length = 396

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLKAQEALY 9
           FL+AQ AL+
Sbjct: 300 FLRAQAALF 308
>XP_006711257
          Length = 469

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  FLKAQEALY 9
          FL+AQ AL+
Sbjct: 24 FLRAQAALF 32
>XP_005244989
          Length = 491

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  FLKAQEALY 9
          FL+AQ AL+
Sbjct: 46 FLRAQAALF 54
>XP_005244988
          Length = 755

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLKAQEALY 9
           FL+AQ AL+
Sbjct: 310 FLRAQAALF 318
>XP_006711256
          Length = 765

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLKAQEALY 9
           FL+AQ AL+
Sbjct: 320 FLRAQAALF 328
>XP_006711255
          Length = 765

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLKAQEALY 9
           FL+AQ AL+
Sbjct: 320 FLRAQAALF 328
>NP_653226
          Length = 1081

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLKAQE 6
           FLKAQE
Sbjct: 556 FLKAQE 561
>NP_001275949
          Length = 797

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KAQEALY 9
           KAQEAL+
Sbjct: 488 KAQEALH 494
>NP_659464
          Length = 814

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KAQEALY 9
           KAQEAL+
Sbjct: 488 KAQEALH 494
>NP_001264004
          Length = 1346

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLKAQEAL 8
           FLK+QE L
Sbjct: 542 FLKSQEPL 549
>XP_005259370
          Length = 943

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLKAQEAL 8
           FLK+QE L
Sbjct: 542 FLKSQEPL 549
>XP_006723477
          Length = 1284

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLKAQEAL 8
           FLK+QE L
Sbjct: 480 FLKSQEPL 487
>XP_005259369
          Length = 1288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLKAQEAL 8
           FLK+QE L
Sbjct: 542 FLKSQEPL 549
>XP_005259368
          Length = 1346

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLKAQEAL 8
           FLK+QE L
Sbjct: 542 FLKSQEPL 549
>XP_005252887
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLKAQEAL 8
           +LK QEAL
Sbjct: 277 YLKLQEAL 284
>XP_005252886
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLKAQEAL 8
           +LK QEAL
Sbjct: 368 YLKLQEAL 375
>XP_005248961
          Length = 842

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KAQEALY 9
           KAQEAL+
Sbjct: 488 KAQEALH 494
>XP_006711799
          Length = 1091

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLKAQE 6
           FLKAQE
Sbjct: 566 FLKAQE 571
>NP_055870
          Length = 585

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLKAQEAL 8
           +LK QEAL
Sbjct: 426 YLKLQEAL 433
>NP_000042
          Length = 3056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FLKAQEAL 8
            +LKAQE +
Sbjct: 1124 YLKAQEGM 1131
>NP_001164564
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LKAQEALY 9
           L+AQ++LY
Sbjct: 214 LEAQDSLY 221
>NP_653236
          Length = 366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LKAQEALY 9
           L+AQ++LY
Sbjct: 299 LEAQDSLY 306
>XP_006725169
          Length = 1368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   FLKAQEA 7
           FLK QEA
Sbjct: 542 FLKEQEA 548
>XP_005271621
          Length = 2708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLKAQEAL 8
           +LKAQE +
Sbjct: 776 YLKAQEGM 783
>XP_006718907
          Length = 3050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FLKAQEAL 8
            +LKAQE +
Sbjct: 1118 YLKAQEGM 1125
>XP_006718906
          Length = 3056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FLKAQEAL 8
            +LKAQE +
Sbjct: 1124 YLKAQEGM 1131
>XP_005271619
          Length = 3056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FLKAQEAL 8
            +LKAQE +
Sbjct: 1124 YLKAQEGM 1131
>XP_005271618
          Length = 3056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FLKAQEAL 8
            +LKAQE +
Sbjct: 1124 YLKAQEGM 1131
>XP_005266097
          Length = 1368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   FLKAQEA 7
           FLK QEA
Sbjct: 542 FLKEQEA 548
>XP_005247172
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LKAQEALY 9
           L+AQ++LY
Sbjct: 214 LEAQDSLY 221
>XP_005247171
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LKAQEALY 9
           L+AQ++LY
Sbjct: 214 LEAQDSLY 221
>XP_005247170
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LKAQEALY 9
           L+AQ++LY
Sbjct: 214 LEAQDSLY 221
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.137    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 382,942
Number of extensions: 577
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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