Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000072                                                              16    5631
NP_689417                                                              16    5631
XP_006720248                                                           16    5631
XP_005267848                                                           16    5631
XP_005273087                                                           16    5631
XP_006711794                                                           16    5631
XP_005273086                                                           16    5631
XP_005273085                                                           16    5631
XP_005273084                                                           16    5631
NP_060413                                                              16    5631
NP_001240781                                                           15    9605
NP_006585                                                              15    9605
NP_001240782                                                           15    9605
NP_001006623                                                           15    9605
NP_001138767                                                           15    9605
NP_839955                                                              15    9605
NP_001020095                                                           15    9605
XP_005265807                                                           15    9605
XP_006714105                                                           15    9605
XP_005265805                                                           15    9605
XP_005265804                                                           15    9605
XP_005265374                                                           15    9605
XP_005270439                                                           15    9605
XP_005270438                                                           15    9605
NP_060731                                                              15    9605
>NP_000072
          Length = 3801

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    FLIMIKLFR 9
            F+I+ KLFR
Sbjct: 995  FMIIQKLFR 1003
>NP_689417
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           IM+K+FR
Sbjct: 582 IMVKIFR 588
>XP_006720248
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           IM+K+FR
Sbjct: 582 IMVKIFR 588
>XP_005267848
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           IM+K+FR
Sbjct: 582 IMVKIFR 588
>XP_005273087
          Length = 2788

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    FLIMIKLFR 9
            F+I+ KLFR
Sbjct: 995  FMIIQKLFR 1003
>XP_006711794
          Length = 3022

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLIMIKLFR 9
           F+I+ KLFR
Sbjct: 216 FMIIQKLFR 224
>XP_005273086
          Length = 3264

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    FLIMIKLFR 9
            F+I+ KLFR
Sbjct: 995  FMIIQKLFR 1003
>XP_005273085
          Length = 3755

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    FLIMIKLFR 9
            F+I+ KLFR
Sbjct: 995  FMIIQKLFR 1003
>XP_005273084
          Length = 3801

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    FLIMIKLFR 9
            F+I+ KLFR
Sbjct: 995  FMIIQKLFR 1003
>NP_060413
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           IM+K+FR
Sbjct: 582 IMVKIFR 588
>NP_001240781
          Length = 739

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLIMIKLF 8
           FLI+ K+F
Sbjct: 259 FLILAKMF 266
>NP_006585
          Length = 739

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLIMIKLF 8
           FLI+ K+F
Sbjct: 259 FLILAKMF 266
>NP_001240782
          Length = 640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLIMIKLF 8
           FLI+ K+F
Sbjct: 160 FLILAKMF 167
>NP_001006623
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   LIMIKLF 8
           ++M+KLF
Sbjct: 221 IVMVKLF 227
>NP_001138767
          Length = 558

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLIMIKLF 8
           FL+ +KLF
Sbjct: 547 FLVPLKLF 554
>NP_839955
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           +MIK+FR
Sbjct: 567 MMIKIFR 573
>NP_001020095
          Length = 894

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           +MIK+FR
Sbjct: 582 MMIKIFR 588
>XP_005265807
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           +MIK+FR
Sbjct: 567 MMIKIFR 573
>XP_006714105
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           +MIK+FR
Sbjct: 585 MMIKIFR 591
>XP_005265805
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           +MIK+FR
Sbjct: 600 MMIKIFR 606
>XP_005265804
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           +MIK+FR
Sbjct: 600 MMIKIFR 606
>XP_005265374
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLIMIKLF 8
           FL+ +KLF
Sbjct: 492 FLVPLKLF 499
>XP_005270439
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLIMIKLF 8
           FLI+ K+F
Sbjct: 259 FLILAKMF 266
>XP_005270438
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLIMIKLF 8
           FLI+ K+F
Sbjct: 259 FLILAKMF 266
>NP_060731
          Length = 894

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IMIKLFR 9
           +MIK+FR
Sbjct: 582 MMIKIFR 588
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.372    0.180    0.511 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 267,529
Number of extensions: 276
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.5 bits)
S2: 28 (15.4 bits)

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