Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           20     390
NP_001257437                                                           20     390
NP_006281                                                              20     390
XP_006715618                                                           20     390
XP_005267176                                                           20     390
NP_002829                                                              16    5631
XP_006711535                                                           16    5631
NP_001034553                                                           16    7355
NP_001244068                                                           16    7355
NP_001099003                                                           16    7355
NP_150229                                                              16    7355
NP_079189                                                              16    7355
NP_004061                                                              16    7355
XP_006710134                                                           16    7355
NP_001036169                                                           16    7355
NP_054706                                                              15    9605
NP_003364                                                              15    9605
NP_387450                                                              15    9605
NP_004352                                                              15    9605
NP_078966                                                              15    9605
XP_005270200                                                           15    9605
XP_005270199                                                           15    9605
>NP_001257436
          Length = 790

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FLFSETTTM 9
           FLFSETT M
Sbjct: 468 FLFSETTAM 476
>NP_001257437
          Length = 790

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FLFSETTTM 9
           FLFSETT M
Sbjct: 468 FLFSETTAM 476
>NP_006281
          Length = 790

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FLFSETTTM 9
           FLFSETT M
Sbjct: 468 FLFSETTAM 476
>XP_006715618
          Length = 577

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FLFSETTTM 9
           FLFSETT M
Sbjct: 255 FLFSETTAM 263
>XP_005267176
          Length = 790

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FLFSETTTM 9
           FLFSETT M
Sbjct: 468 FLFSETTAM 476
>NP_002829
          Length = 1306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FSETTT 8
          FSETTT
Sbjct: 69 FSETTT 74
>XP_006711535
          Length = 1258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FSETTT 8
          FSETTT
Sbjct: 69 FSETTT 74
>NP_001034553
          Length = 1323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LFSETTTM 9
           LFS+T+T+
Sbjct: 344 LFSDTSTL 351
>NP_001244068
          Length = 644

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LFSETT 7
           LFSETT
Sbjct: 592 LFSETT 597
>NP_001099003
          Length = 783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LFSETTTM 9
           +FSET+T+
Sbjct: 340 VFSETSTL 347
>NP_150229
          Length = 1359

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FLFSETTTM 9
           F+F++TT M
Sbjct: 871 FVFTKTTLM 879
>NP_079189
          Length = 1026

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LFSETTTM 9
           +FSET+T+
Sbjct: 340 VFSETSTL 347
>NP_004061
          Length = 687

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LFSETT 7
           LFSETT
Sbjct: 635 LFSETT 640
>XP_006710134
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LFSETT 7
           LFSETT
Sbjct: 282 LFSETT 287
>NP_001036169
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LFSETT 7
           LFSETT
Sbjct: 634 LFSETT 639
>NP_054706
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LFSETTT 8
           +FS+TTT
Sbjct: 598 VFSDTTT 604
>NP_003364
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LFSETTT 8
           +FS+TTT
Sbjct: 598 VFSDTTT 604
>NP_387450
          Length = 785

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FSETTTM 9
           FS+TTT+
Sbjct: 358 FSDTTTV 364
>NP_004352
          Length = 785

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FSETTTM 9
           FS+TTT+
Sbjct: 358 FSDTTTV 364
>NP_078966
          Length = 14507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FLFSETTTM 9
            F +SE TT+
Sbjct: 4129 FTYSEKTTL 4137
>XP_005270200
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LFSETTT 8
           +FS+TTT
Sbjct: 599 VFSDTTT 605
>XP_005270199
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LFSETTT 8
           +FS+TTT
Sbjct: 599 VFSDTTT 605
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.129    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 412,169
Number of extensions: 679
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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