Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              21     229
XP_005273257                                                           21     229
NP_003117                                                              18    1135
XP_005260978                                                           18    1135
NP_079046                                                              17    2528
XP_006712828                                                           17    2528
XP_006712827                                                           17    2528
XP_006712826                                                           17    2528
XP_006712825                                                           17    2528
NP_612450                                                              17    4312
XP_006715390                                                           17    4312
NP_689609                                                              16    5631
XP_006713034                                                           16    5631
XP_006713033                                                           16    5631
XP_006713032                                                           16    5631
XP_005264924                                                           16    5631
YP_003024028                                                           16    5631
NP_036413                                                              16    7355
NP_000304                                                              16    7355
NP_006721                                                              16    7355
NP_001009932                                                           16    7355
NP_001009933                                                           16    7355
NP_001009934                                                           16    7355
NP_001269512                                                           16    7355
NP_001269514                                                           16    7355
NP_001269511                                                           16    7355
NP_001269513                                                           16    7355
NP_115724                                                              16    7355
XP_005277886                                                           16    7355
XP_005250379                                                           16    7355
XP_005247683                                                           16    7355
XP_006710765                                                           16    7355
XP_005244815                                                           16    7355
NP_000234                                                              15    9605
NP_001185465                                                           15    9605
NP_067675                                                              15    9605
NP_079471                                                              15    9605
NP_071400                                                              15    9605
NP_055947                                                              15    9605
XP_006724655                                                           15    9605
XP_005257947                                                           15    9605
XP_006720942                                                           15    9605
XP_006716559                                                           15    9605
XP_005249729                                                           15    9605
>NP_064450
          Length = 1722

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    FLETRAYFF 9
            FLET AYFF
Sbjct: 1638 FLETTAYFF 1646
>XP_005273257
          Length = 1726

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    FLETRAYFF 9
            FLET AYFF
Sbjct: 1642 FLETTAYFF 1650
>NP_003117
          Length = 2419

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2    LETRAYF 8
            LETRAYF
Sbjct: 2174 LETRAYF 2180
>XP_005260978
          Length = 483

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLETRAYFF 9
           FL+T AY+F
Sbjct: 354 FLDTSAYYF 362
>NP_079046
          Length = 407

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRAYFF 9
           TRAYFF
Sbjct: 337 TRAYFF 342
>XP_006712828
          Length = 357

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRAYFF 9
           TRAYFF
Sbjct: 287 TRAYFF 292
>XP_006712827
          Length = 362

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRAYFF 9
           TRAYFF
Sbjct: 292 TRAYFF 297
>XP_006712826
          Length = 407

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRAYFF 9
           TRAYFF
Sbjct: 337 TRAYFF 342
>XP_006712825
          Length = 455

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRAYFF 9
           TRAYFF
Sbjct: 385 TRAYFF 390
>NP_612450
          Length = 522

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FLETRAYFF 9
           +  TRAY+F
Sbjct: 242 YSNTRAYYF 250
>XP_006715390
          Length = 445

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FLETRAYFF 9
           +  TRAY+F
Sbjct: 242 YSNTRAYYF 250
>NP_689609
          Length = 284

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRAYFF 9
           TR+YFF
Sbjct: 217 TRSYFF 222
>XP_006713034
          Length = 239

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRAYFF 9
           TR+YFF
Sbjct: 172 TRSYFF 177
>XP_006713033
          Length = 284

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRAYFF 9
           TR+YFF
Sbjct: 217 TRSYFF 222
>XP_006713032
          Length = 284

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRAYFF 9
           TR+YFF
Sbjct: 217 TRSYFF 222
>XP_005264924
          Length = 284

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRAYFF 9
           TR+YFF
Sbjct: 217 TRSYFF 222
>YP_003024028
          Length = 513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LETRAYF 8
           ++TRAYF
Sbjct: 299 VDTRAYF 305
>NP_036413
          Length = 630

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  ETRAYFF 9
          ET+ YFF
Sbjct: 79 ETQQYFF 85
>NP_000304
          Length = 676

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   LETRAYF 8
           LET+ YF
Sbjct: 421 LETKVYF 427
>NP_006721
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   FLETRAYFF 9
           ++ET  YF+
Sbjct: 95  YMETYVYFY 103
>NP_001009932
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   FLETRAYFF 9
           ++ET  YF+
Sbjct: 95  YMETYVYFY 103
>NP_001009933
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   FLETRAYFF 9
           ++ET  YF+
Sbjct: 95  YMETYVYFY 103
>NP_001009934
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   FLETRAYFF 9
           ++ET  YF+
Sbjct: 95  YMETYVYFY 103
>NP_001269512
          Length = 433

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ETRAYFF 9
           E+RA+FF
Sbjct: 324 ESRAHFF 330
>NP_001269514
          Length = 450

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ETRAYFF 9
           E+RA+FF
Sbjct: 333 ESRAHFF 339
>NP_001269511
          Length = 442

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ETRAYFF 9
           E+RA+FF
Sbjct: 333 ESRAHFF 339
>NP_001269513
          Length = 341

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ETRAYFF 9
           E+RA+FF
Sbjct: 232 ESRAHFF 238
>NP_115724
          Length = 442

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ETRAYFF 9
           E+RA+FF
Sbjct: 333 ESRAHFF 339
>XP_005277886
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   FLETRAYFF 9
           ++ET  YF+
Sbjct: 95  YMETYVYFY 103
>XP_005250379
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  ETRAYFF 9
          ET+ YFF
Sbjct: 79 ETQQYFF 85
>XP_005247683
          Length = 708

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   LETRAYF 8
           LET+ YF
Sbjct: 453 LETKVYF 459
>XP_006710765
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ETRAYFF 9
           E+RA+FF
Sbjct: 232 ESRAHFF 238
>XP_005244815
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ETRAYFF 9
           E+RA+FF
Sbjct: 333 ESRAHFF 339
>NP_000234
          Length = 781

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FLETRAYFF 9
           FLE + +FF
Sbjct: 472 FLEQQEHFF 480
>NP_001185465
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FLETRAYFF 9
           FLE + +FF
Sbjct: 261 FLEQQEHFF 269
>NP_067675
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   ETRAYFF 9
           ET+AY F
Sbjct: 123 ETKAYVF 129
>NP_079471
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   ETRAYFF 9
           ET+AY F
Sbjct: 153 ETKAYVF 159
>NP_071400
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FLETRAYF 8
           F+ET  YF
Sbjct: 455 FMETTDYF 462
>NP_055947
          Length = 957

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FLETRAY 7
           FLE +AY
Sbjct: 672 FLENKAY 678
>XP_006724655
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FLETRAYF 8
           F+ET  YF
Sbjct: 455 FMETTDYF 462
>XP_005257947
          Length = 1935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   ETRAYF 8
           ETR YF
Sbjct: 717 ETRVYF 722
>XP_006720942
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FLETRAYFF 9
           FLE + +FF
Sbjct: 471 FLEQQEHFF 479
>XP_006716559
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  ETRAYFF 9
          ET+AY F
Sbjct: 43 ETKAYVF 49
>XP_005249729
          Length = 968

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FLETRAY 7
           FLE +AY
Sbjct: 683 FLENKAY 689
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.353    0.159    0.511 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 453,845
Number of extensions: 663
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)

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