Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              18    1482
NP_001276018                                                           17    4312
NP_079048                                                              17    4312
NP_001276019                                                           17    4312
NP_001276017                                                           17    4312
NP_001276020                                                           17    4312
XP_005271279                                                           17    4312
XP_005271277                                                           17    4312
XP_005271276                                                           17    4312
XP_005271275                                                           17    4312
XP_005271273                                                           17    4312
NP_954868                                                              16    5631
NP_954869                                                              16    5631
NP_001277153                                                           16    5631
NP_954870                                                              16    5631
NP_954863                                                              16    5631
NP_001229759                                                           16    7355
NP_068586                                                              16    7355
NP_001229757                                                           16    7355
XP_005249324                                                           16    7355
XP_005249323                                                           16    7355
NP_612480                                                              16    7355
>NP_115803
          Length = 1156

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FLDIKIIRV 9
           FLDIK IRV
Sbjct: 200 FLDIKRIRV 208
>NP_001276018
          Length = 1123

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           FLD+ IIR
Sbjct: 730 FLDLSIIR 737
>NP_079048
          Length = 1142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           FLD+ IIR
Sbjct: 749 FLDLSIIR 756
>NP_001276019
          Length = 1142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           FLD+ IIR
Sbjct: 749 FLDLSIIR 756
>NP_001276017
          Length = 1142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           FLD+ IIR
Sbjct: 749 FLDLSIIR 756
>NP_001276020
          Length = 782

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           FLD+ IIR
Sbjct: 389 FLDLSIIR 396
>XP_005271279
          Length = 782

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           FLD+ IIR
Sbjct: 389 FLDLSIIR 396
>XP_005271277
          Length = 951

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           FLD+ IIR
Sbjct: 558 FLDLSIIR 565
>XP_005271276
          Length = 1067

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           FLD+ IIR
Sbjct: 674 FLDLSIIR 681
>XP_005271275
          Length = 1067

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           FLD+ IIR
Sbjct: 674 FLDLSIIR 681
>XP_005271273
          Length = 1130

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           FLD+ IIR
Sbjct: 737 FLDLSIIR 744
>NP_954868
          Length = 533

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           F DIK++R
Sbjct: 160 FFDIKLLR 167
>NP_954869
          Length = 513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           F DIK++R
Sbjct: 140 FFDIKLLR 147
>NP_001277153
          Length = 413

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  FLDIKIIR 8
          F DIK++R
Sbjct: 40 FFDIKLLR 47
>NP_954870
          Length = 551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           F DIK++R
Sbjct: 178 FFDIKLLR 185
>NP_954863
          Length = 522

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FLDIKIIR 8
           F DIK++R
Sbjct: 160 FFDIKLLR 167
>NP_001229759
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DIKIIRV 9
           D+KIIRV
Sbjct: 106 DMKIIRV 112
>NP_068586
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DIKIIRV 9
           D+KIIRV
Sbjct: 106 DMKIIRV 112
>NP_001229757
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DIKIIRV 9
           D+KIIRV
Sbjct: 133 DMKIIRV 139
>XP_005249324
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DIKIIRV 9
           D+KIIRV
Sbjct: 106 DMKIIRV 112
>XP_005249323
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DIKIIRV 9
           D+KIIRV
Sbjct: 106 DMKIIRV 112
>NP_612480
          Length = 381

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LDIKIIRV 9
           LD+++IRV
Sbjct: 321 LDLQVIRV 328
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.353    0.174    0.471 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 406,204
Number of extensions: 572
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)

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