Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_075069                                                              17    4312
NP_001162195                                                           17    4312
XP_006718731                                                           17    4312
XP_006718730                                                           17    4312
NP_001265723                                                           16    5631
NP_005963                                                              16    5631
NP_001265724                                                           16    5631
XP_005277701                                                           16    5631
XP_006717608                                                           16    5631
NP_001750                                                              15    9605
NP_203752                                                              15    9605
NP_001139381                                                           15    9605
XP_005253870                                                           15    9605
XP_006712877                                                           15    9605
XP_006712876                                                           15    9605
XP_006712875                                                           15    9605
XP_006712874                                                           15    9605
XP_006712873                                                           15    9605
XP_006712872                                                           15    9605
XP_005246976                                                           15    9605
XP_005246975                                                           15    9605
>NP_075069
          Length = 458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLASKIVST 9
           +LASK+V T
Sbjct: 352 YLASKLVET 360
>NP_001162195
          Length = 436

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLASKIVST 9
           +LASK+V T
Sbjct: 330 YLASKLVET 338
>XP_006718731
          Length = 330

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLASKIVST 9
           +LASK+V T
Sbjct: 224 YLASKLVET 232
>XP_006718730
          Length = 343

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FLASKIVST 9
           +LASK+V T
Sbjct: 237 YLASKLVET 245
>NP_001265723
          Length = 375

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FLASKIVST 9
           FLA K+V T
Sbjct: 194 FLADKVVCT 202
>NP_005963
          Length = 375

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FLASKIVST 9
           FLA K+V T
Sbjct: 194 FLADKVVCT 202
>NP_001265724
          Length = 375

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FLASKIVST 9
           FLA K+V T
Sbjct: 194 FLADKVVCT 202
>XP_005277701
          Length = 375

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FLASKIVST 9
           FLA K+V T
Sbjct: 194 FLADKVVCT 202
>XP_006717608
          Length = 375

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FLASKIVST 9
           FLA K+V T
Sbjct: 194 FLADKVVCT 202
>NP_001750
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FLASKIVST 9
           FLASK+  T
Sbjct: 107 FLASKLKET 115
>NP_203752
          Length = 1861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLASKIVS 8
           +LASK+V+
Sbjct: 497 YLASKVVA 504
>NP_001139381
          Length = 1390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLASKIVS 8
           +LASK+V+
Sbjct: 489 YLASKVVA 496
>XP_005253870
          Length = 217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FLASKIVST 9
           FLASK+  T
Sbjct: 107 FLASKLKET 115
>XP_006712877
          Length = 1677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLASKIVS 8
           +LASK+V+
Sbjct: 313 YLASKVVA 320
>XP_006712876
          Length = 1783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLASKIVS 8
           +LASK+V+
Sbjct: 419 YLASKVVA 426
>XP_006712875
          Length = 1826

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLASKIVS 8
           +LASK+V+
Sbjct: 462 YLASKVVA 469
>XP_006712874
          Length = 1836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLASKIVS 8
           +LASK+V+
Sbjct: 472 YLASKVVA 479
>XP_006712873
          Length = 1861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLASKIVS 8
           +LASK+V+
Sbjct: 497 YLASKVVA 504
>XP_006712872
          Length = 1861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLASKIVS 8
           +LASK+V+
Sbjct: 497 YLASKVVA 504
>XP_005246976
          Length = 1864

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLASKIVS 8
           +LASK+V+
Sbjct: 500 YLASKVVA 507
>XP_005246975
          Length = 1871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLASKIVS 8
           +LASK+V+
Sbjct: 507 YLASKVVA 514
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.128    0.304 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 218,717
Number of extensions: 220
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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