Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_006222                                                              18    1482
NP_056162                                                              17    3301
NP_004501                                                              17    4312
NP_001004106                                                           17    4312
NP_004500                                                              17    4312
NP_001004105                                                           17    4312
NP_536349                                                              17    4312
NP_002073                                                              17    4312
NP_001171944                                                           17    4312
NP_001070254                                                           17    4312
XP_006718388                                                           17    4312
XP_006718387                                                           17    4312
XP_006718386                                                           17    4312
XP_006714923                                                           17    4312
XP_006714922                                                           17    4312
XP_006714921                                                           17    4312
XP_006712552                                                           17    4312
XP_006712551                                                           17    4312
XP_006712550                                                           17    4312
XP_006712549                                                           17    4312
XP_005246582                                                           17    4312
NP_071325                                                              16    7355
NP_998778                                                              16    7355
XP_006713920                                                           16    7355
XP_006713919                                                           16    7355

>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FKRLGASVA 9
            FKRLGAS+A
Sbjct: 1073 FKRLGASLA 1081



>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FKRLGASVA 9
            FKRLGAS+A
Sbjct: 1073 FKRLGASLA 1081



>NP_006222
          Length = 2286

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1    FKRLGASV 8
            FKRLG+SV
Sbjct: 1856 FKRLGSSV 1863



>NP_056162
          Length = 1052

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   FKRLGASV 8
           FKR GASV
Sbjct: 715 FKRRGASV 722



>NP_004501
          Length = 549

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKRLGAS 7
           FKR+GAS
Sbjct: 103 FKRVGAS 109



>NP_001004106
          Length = 576

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FKRLGASV 8
           FKRLGA +
Sbjct: 453 FKRLGAGM 460



>NP_004500
          Length = 689

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKRLGAS 7
           FKR+GAS
Sbjct: 103 FKRVGAS 109



>NP_001004105
          Length = 560

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FKRLGASV 8
           FKRLGA +
Sbjct: 453 FKRLGAGM 460



>NP_536349
          Length = 713

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKRLGAS 7
           FKR+GAS
Sbjct: 103 FKRVGAS 109



>NP_002073
          Length = 589

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FKRLGASV 8
           FKRLGA +
Sbjct: 453 FKRLGAGM 460



>NP_001171944
          Length = 555

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKRLGAS 7
           FKR+GAS
Sbjct: 109 FKRVGAS 115



>NP_001070254
          Length = 1735

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FKRLGASV 8
           F+RLG+SV
Sbjct: 115 FERLGSSV 122



>XP_006718388
          Length = 1331

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FKRLGASV 8
           F+RLG+SV
Sbjct: 178 FERLGSSV 185



>XP_006718387
          Length = 1798

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FKRLGASV 8
           F+RLG+SV
Sbjct: 178 FERLGSSV 185



>XP_006718386
          Length = 1822

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FKRLGASV 8
           F+RLG+SV
Sbjct: 178 FERLGSSV 185



>XP_006714923
          Length = 324

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FKRLGASV 8
           FKRLGA +
Sbjct: 188 FKRLGAGM 195



>XP_006714922
          Length = 557

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FKRLGASV 8
           FKRLGA +
Sbjct: 421 FKRLGAGM 428



>XP_006714921
          Length = 559

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FKRLGASV 8
           FKRLGA +
Sbjct: 423 FKRLGAGM 430



>XP_006712552
          Length = 648

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKRLGAS 7
           FKR+GAS
Sbjct: 109 FKRVGAS 115



>XP_006712551
          Length = 669

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKRLGAS 7
           FKR+GAS
Sbjct: 109 FKRVGAS 115



>XP_006712550
          Length = 695

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKRLGAS 7
           FKR+GAS
Sbjct: 109 FKRVGAS 115



>XP_006712549
          Length = 713

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKRLGAS 7
           FKR+GAS
Sbjct: 103 FKRVGAS 109



>XP_005246582
          Length = 719

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKRLGAS 7
           FKR+GAS
Sbjct: 109 FKRVGAS 115



>NP_071325
          Length = 701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRLGASVA 9
           KR G+SVA
Sbjct: 313 KRFGSSVA 320



>NP_998778
          Length = 701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRLGASVA 9
           KR G+SVA
Sbjct: 313 KRFGSSVA 320



>XP_006713920
          Length = 701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRLGASVA 9
           KR G+SVA
Sbjct: 313 KRFGSSVA 320



>XP_006713919
          Length = 701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRLGASVA 9
           KR G+SVA
Sbjct: 313 KRFGSSVA 320



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.136    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 384,787
Number of extensions: 475
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)