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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              18    1135
NP_001087241                                                           18    1135
XP_005265799                                                           18    1135
XP_005265798                                                           18    1135
XP_005265797                                                           18    1135
NP_055409                                                              17    4312
XP_005265951                                                           17    4312
NP_000777                                                              16    5631
NP_001139624                                                           16    5631
NP_065810                                                              16    5631
XP_006720684                                                           16    5631
NP_777551                                                              16    7355
XP_005267375                                                           16    7355
XP_005267374                                                           16    7355
NP_003038                                                              15    9605
NP_061836                                                              15    9605
NP_631894                                                              15    9605
NP_542453                                                              15    9605
NP_079204                                                              15    9605
NP_387505                                                              15    9605
NP_061121                                                              15    9605
NP_001275519                                                           15    9605
XP_006726853                                                           15    9605
XP_005261051                                                           15    9605
XP_006723596                                                           15    9605
XP_006718039                                                           15    9605
XP_005265380                                                           15    9605
XP_006710111                                                           15    9605

>NP_000213
          Length = 976

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           FKQ VQLIE
Sbjct: 917 FKQIVQLIE 925



>NP_001087241
          Length = 972

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           FKQ VQLIE
Sbjct: 913 FKQIVQLIE 921



>XP_005265799
          Length = 973

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           FKQ VQLIE
Sbjct: 914 FKQIVQLIE 922



>XP_005265798
          Length = 976

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           FKQ VQLIE
Sbjct: 917 FKQIVQLIE 925



>XP_005265797
          Length = 977

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           FKQ VQLIE
Sbjct: 918 FKQIVQLIE 926



>NP_055409
          Length = 554

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           FKQ++ LIE
Sbjct: 225 FKQNLHLIE 233



>XP_005265951
          Length = 554

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           FKQ++ LIE
Sbjct: 225 FKQNLHLIE 233



>NP_000777
          Length = 509

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKQSVQLIE 9
           FKQ V +IE
Sbjct: 174 FKQHVSIIE 182



>NP_001139624
          Length = 404

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FKQSVQLIE 9
          FKQ V +IE
Sbjct: 69 FKQHVSIIE 77



>NP_065810
          Length = 4700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           +KQ +QL+E
Sbjct: 196 YKQVIQLLE 204



>XP_006720684
          Length = 4713

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           +KQ +QL+E
Sbjct: 196 YKQVIQLLE 204



>NP_777551
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           F QS++L+E
Sbjct: 94  FSQSLELLE 102



>XP_005267375
          Length = 274

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           F QS++L+E
Sbjct: 94  FSQSLELLE 102



>XP_005267374
          Length = 319

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           F QS++L+E
Sbjct: 94  FSQSLELLE 102



>NP_003038
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KQSVQLIE 9
           KQ+++L+E
Sbjct: 583 KQAIELVE 590



>NP_061836
          Length = 2320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FKQSVQLIE 9
            F+Q V L+E
Sbjct: 1338 FRQPVDLVE 1346



>NP_631894
          Length = 1226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           ++QS+ LIE
Sbjct: 316 YRQSLSLIE 324



>NP_542453
          Length = 1223

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           ++QS+ LIE
Sbjct: 316 YRQSLSLIE 324



>NP_079204
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           FK ++QL++
Sbjct: 267 FKNAIQLLQ 275



>NP_387505
          Length = 2269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FKQSVQLIE 9
            F+Q V L+E
Sbjct: 1338 FRQPVDLVE 1346



>NP_061121
          Length = 754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKQSVQLIE 9
           F Q+ QLIE
Sbjct: 588 FNQNSQLIE 596



>NP_001275519
          Length = 754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKQSVQLIE 9
           F Q+ QLIE
Sbjct: 588 FNQNSQLIE 596



>XP_006726853
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FKQSVQLIE 9
           F QS  LIE
Sbjct: 552 FSQSTHLIE 560



>XP_005261051
          Length = 2242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FKQSVQLIE 9
            F+Q V L+E
Sbjct: 1260 FRQPVDLVE 1268



>XP_006723596
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FKQSVQLIE 9
           F QS  LIE
Sbjct: 552 FSQSTHLIE 560



>XP_006718039
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FKQSVQLIE 9
           FK ++QL++
Sbjct: 267 FKNAIQLLQ 275



>XP_005265380
          Length = 753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKQSVQLIE 9
           F Q+ QLIE
Sbjct: 587 FNQNSQLIE 595



>XP_006710111
          Length = 566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FKQSVQLIE 9
           F QS  LIE
Sbjct: 411 FSQSTHLIE 419



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.135    0.324 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 300,340
Number of extensions: 672
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)