Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     299
NP_001091102                                                           17    4312
XP_006717115                                                           17    4312
NP_001091105                                                           17    4312
NP_001481                                                              17    4312
NP_001091103                                                           17    4312
NP_001091104                                                           17    4312
NP_063945                                                              16    7355
NP_004414                                                              16    7355
NP_004412                                                              16    7355
NP_001127928                                                           16    7355
NP_659474                                                              16    7355
NP_004413                                                              16    7355
XP_006721532                                                           16    7355
XP_005256559                                                           16    7355
XP_006717250                                                           16    7355
XP_005266151                                                           16    7355
XP_005247229                                                           16    7355
XP_005244790                                                           16    7355
XP_005244789                                                           16    7355
XP_005244788                                                           16    7355
NP_001229825                                                           15    9605
NP_001229826                                                           15    9605
NP_055477                                                              15    9605
NP_001129501                                                           15    9605
NP_722523                                                              15    9605
NP_065201                                                              15    9605
XP_005261924                                                           15    9605
XP_006724450                                                           15    9605
XP_006724449                                                           15    9605
XP_005261923                                                           15    9605
XP_006724448                                                           15    9605
XP_006724447                                                           15    9605
XP_005261922                                                           15    9605
XP_005261921                                                           15    9605
XP_005261920                                                           15    9605
XP_005261919                                                           15    9605
XP_005248797                                                           15    9605
XP_005248796                                                           15    9605
XP_005248795                                                           15    9605
>NP_004351
          Length = 882

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FKGSVMKGA 9
           FKGSVM+GA
Sbjct: 267 FKGSVMEGA 275
>NP_001091102
          Length = 428

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           F+G V KGA
Sbjct: 359 FEGDVSKGA 367
>XP_006717115
          Length = 428

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           F+G V KGA
Sbjct: 359 FEGDVSKGA 367
>NP_001091105
          Length = 428

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           F+G V KGA
Sbjct: 359 FEGDVSKGA 367
>NP_001481
          Length = 428

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           F+G V KGA
Sbjct: 359 FEGDVSKGA 367
>NP_001091103
          Length = 428

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           F+G V KGA
Sbjct: 359 FEGDVSKGA 367
>NP_001091104
          Length = 428

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           F+G V KGA
Sbjct: 359 FEGDVSKGA 367
>NP_063945
          Length = 644

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KGSVMKG 8
           KGS+ KG
Sbjct: 516 KGSIFKG 522
>NP_004414
          Length = 716

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSVMKG 8
           GS+MKG
Sbjct: 279 GSIMKG 284
>NP_004412
          Length = 670

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSVMKG 8
           GS+MKG
Sbjct: 281 GSIMKG 286
>NP_001127928
          Length = 559

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FKGSVMKG 8
           FKG  +KG
Sbjct: 264 FKGGYLKG 271
>NP_659474
          Length = 559

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FKGSVMKG 8
           FKG  +KG
Sbjct: 264 FKGGYLKG 271
>NP_004413
          Length = 736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSVMKG 8
           GS+MKG
Sbjct: 297 GSIMKG 302
>XP_006721532
          Length = 523

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSVMKG 8
           GS+MKG
Sbjct: 297 GSIMKG 302
>XP_005256559
          Length = 732

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSVMKG 8
           GS+MKG
Sbjct: 293 GSIMKG 298
>XP_006717250
          Length = 610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KGSVMKG 8
           KGS+ KG
Sbjct: 482 KGSIFKG 488
>XP_005266151
          Length = 643

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KGSVMKG 8
           KGS+ KG
Sbjct: 515 KGSIFKG 521
>XP_005247229
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSVMKG 8
           GS+MKG
Sbjct: 279 GSIMKG 284
>XP_005244790
          Length = 549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSVMKG 8
           GS+MKG
Sbjct: 281 GSIMKG 286
>XP_005244789
          Length = 574

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSVMKG 8
           GS+MKG
Sbjct: 281 GSIMKG 286
>XP_005244788
          Length = 695

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GSVMKG 8
           GS+MKG
Sbjct: 281 GSIMKG 286
>NP_001229825
          Length = 1603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    KGSVMKG 8
            KG+ MKG
Sbjct: 1008 KGTAMKG 1014
>NP_001229826
          Length = 1503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KGSVMKG 8
           KG+ MKG
Sbjct: 930 KGTAMKG 936
>NP_055477
          Length = 1572

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    KGSVMKG 8
            KG+ MKG
Sbjct: 999  KGTAMKG 1005
>NP_001129501
          Length = 1594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    KGSVMKG 8
            KG+ MKG
Sbjct: 999  KGTAMKG 1005
>NP_722523
          Length = 946

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           FK + M+GA
Sbjct: 514 FKSTTMEGA 522
>NP_065201
          Length = 893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           FK + M+GA
Sbjct: 461 FKSTTMEGA 469
>XP_005261924
          Length = 1176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    KGSVMKG 8
            KG+ MKG
Sbjct: 1008 KGTAMKG 1014
>XP_006724450
          Length = 1187

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    KGSVMKG 8
            KG+ MKG
Sbjct: 1008 KGTAMKG 1014
>XP_006724449
          Length = 1198

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    KGSVMKG 8
            KG+ MKG
Sbjct: 1008 KGTAMKG 1014
>XP_005261923
          Length = 1307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    KGSVMKG 8
            KG+ MKG
Sbjct: 1008 KGTAMKG 1014
>XP_006724448
          Length = 1490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KGSVMKG 8
           KG+ MKG
Sbjct: 895 KGTAMKG 901
>XP_006724447
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KGSVMKG 8
           KG+ MKG
Sbjct: 921 KGTAMKG 927
>XP_005261922
          Length = 1525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KGSVMKG 8
           KG+ MKG
Sbjct: 930 KGTAMKG 936
>XP_005261921
          Length = 1581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    KGSVMKG 8
            KG+ MKG
Sbjct: 1008 KGTAMKG 1014
>XP_005261920
          Length = 1594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    KGSVMKG 8
            KG+ MKG
Sbjct: 999  KGTAMKG 1005
>XP_005261919
          Length = 1603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    KGSVMKG 8
            KG+ MKG
Sbjct: 1008 KGTAMKG 1014
>XP_005248797
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           FK + M+GA
Sbjct: 470 FKSTTMEGA 478
>XP_005248796
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           FK + M+GA
Sbjct: 505 FKSTTMEGA 513
>XP_005248795
          Length = 945

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FKGSVMKGA 9
           FK + M+GA
Sbjct: 513 FKSTTMEGA 521
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.134    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 499,946
Number of extensions: 1240
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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