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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001121619                                                           19     665
NP_000258                                                              19     665
NP_001035957                                                           19     665
XP_006721991                                                           19     665
XP_006721990                                                           19     665
XP_006721989                                                           19     665
XP_005258041                                                           19     665
XP_006721988                                                           19     665
XP_006721987                                                           19     665
XP_006721986                                                           19     665
XP_005258040                                                           19     665
XP_006721985                                                           19     665
NP_008987                                                              18    1935
NP_001265368                                                           18    1935
XP_006723054                                                           18    1935
NP_004256                                                              16    7355
NP_001268430                                                           16    7355
NP_001268431                                                           16    7355
NP_055952                                                              16    7355
XP_005250906                                                           16    7355
NP_000676                                                              15    9605
NP_033611                                                              15    9605
NP_114438                                                              15    9605
NP_004826                                                              15    9605
NP_114038                                                              15    9605
NP_001127891                                                           15    9605
XP_005271302                                                           15    9605
XP_005271301                                                           15    9605
XP_005271298                                                           15    9605
NP_001127892                                                           15    9605
NP_003494                                                              15    9605

>NP_001121619
          Length = 593

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>NP_000258
          Length = 2818

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>NP_001035957
          Length = 2839

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>XP_006721991
          Length = 1632

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>XP_006721990
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>XP_006721989
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>XP_005258041
          Length = 2836

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>XP_006721988
          Length = 2846

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>XP_006721987
          Length = 2849

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>XP_006721986
          Length = 2854

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 188 FKALKKVAQ 196



>XP_005258040
          Length = 2857

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>XP_006721985
          Length = 2867

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKALKK AQ
Sbjct: 201 FKALKKVAQ 209



>NP_008987
          Length = 674

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKA KKAAQ
Sbjct: 590 FKADKKAAQ 598



>NP_001265368
          Length = 612

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKA KKAAQ
Sbjct: 528 FKADKKAAQ 536



>XP_006723054
          Length = 445

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           FKA KKAAQ
Sbjct: 361 FKADKKAAQ 369



>NP_004256
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FKALKKAAQ 9
           F+AL+K A+
Sbjct: 113 FRALRKTAE 121



>NP_001268430
          Length = 422

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FKALKKAAQ 9
          F+AL+K A+
Sbjct: 91 FRALRKTAE 99



>NP_001268431
          Length = 413

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FKALKKAAQ 9
          F+AL+K A+
Sbjct: 82 FRALRKTAE 90



>NP_055952
          Length = 821

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKALKKA 7
           F+ALKKA
Sbjct: 728 FEALKKA 734



>XP_005250906
          Length = 785

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKALKKA 7
           F+ALKKA
Sbjct: 692 FEALKKA 698



>NP_000676
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKALKKA 7
           +KALKKA
Sbjct: 219 WKALKKA 225



>NP_033611
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKALKKA 7
           +KALKKA
Sbjct: 219 WKALKKA 225



>NP_114438
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKALKKA 7
           +KALKKA
Sbjct: 248 WKALKKA 254



>NP_004826
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKALKKA 7
           +KALKKA
Sbjct: 254 WKALKKA 260



>NP_114038
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FKALKKA 7
           +KALKKA
Sbjct: 254 WKALKKA 260



>NP_001127891
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKALKKAAQ 9
           FKALK+  Q
Sbjct: 162 FKALKRIHQ 170



>XP_005271302
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKALKKAAQ 9
           FKALK+  Q
Sbjct: 162 FKALKRIHQ 170



>XP_005271301
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKALKKAAQ 9
           FKALK+  Q
Sbjct: 162 FKALKRIHQ 170



>XP_005271298
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKALKKAAQ 9
           FKALK+  Q
Sbjct: 162 FKALKRIHQ 170



>NP_001127892
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKALKKAAQ 9
           FKALK+  Q
Sbjct: 162 FKALKRIHQ 170



>NP_003494
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FKALKKAAQ 9
           FKALK+  Q
Sbjct: 162 FKALKRIHQ 170



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.121    0.297 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 272,712
Number of extensions: 371
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)